Method: The B.1.1.7 Spike we used carries the mutations found in GISAID Accession Number EPI_ISL 668152: del 69-70, del145, N501Y, A570D, D614G, P681H, T716I, S982A, and D1118H.
Structural modelling of SARS-CoV-2 alpha variant (B.1.1.7) suggests enhanced furin binding and infectivity.
Introduction: Furthermore, to understand the stability of the S-protein P681H mutation, compared the S-protein B.1.1.7 variant upon interaction with furin, both states were subjected to 100ns molecular dynamic (MD) simulations.
Introduction: Hence, in this study, we compared the S-protein-B.1.1.7 variant structure in complex with furin with the S-protein P681H-furin complex to determine if the B.1.1.7 variant or the P681H mutation affected furin cleavage.
Introduction: In addition, we calculated the binding affinity of furin to S-protein-P681H and S-protein-B.1.1.7 variant using Generalized-Born surface area molecular mechanics (MM/GBSA).|
Conformational Variability Correlation Prediction of Transmissibility and Neutralization Escape Ability for Multiple Mutation SARS-CoV-2 Strains using SSSCPreds.
Result: B.1.1.7 and B.1.617.2 strains have the P681H/R mutation sites at the furin cleavage site of SARS-CoV-2.
Result: The sequence flexibility/rigidity map patterns of the P681H/R mutation sites are more flexible than that of the wild-type strain.
Figure: Sequence flexibility/rigidity maps of all of the single amino acidmutations at the P681H/R mutation sites (blue: identical alpha-helix-typeconformations; red: identical beta-sheet-type conformations; andgreen: identical other-type conformations).
Transformations, Lineage Comparisons, and Analysis of Down-to-Up Protomer States of Variants of the SARS-CoV-2 Prefusion Spike Protein, Including the UK Variant B.1.1.7.
8Discussion: P681H is one of the mutations in the VIC alpha, while P681R is one of the mutations in the lineage A.23.1, which is identified as a ""variant under monitoring"" (, Accessed on 14 June 2021)."
Mutation hotspots and spatiotemporal distribution of SARS-CoV-2 lineages in Brazil, February 2020-2021.
Introduction: L452R, E484K, N501Y, P681H/R) have been positively selected, since they may confer adaptive advantages leading to convergent evolution in different lineages spreading across multiple countries.
Introduction: The former (B.1.1.7) emerged in England in mid-September 2020 and it is characterized by 14 lineage-specific amino acid substitutions, especially N501Y (a key contact residue interacting with hACE2) and P681H (one of four amino acids comprising the insertion that creates a novel furin cleavage site between S1 and S2).
Molecular Evolution and Epidemiological Characteristics of SARS COV-2 in (Northwestern) Poland.
Result: Co-existence of DeltaH69V70, DeltaY144, P681H, T716I, S982A, A570D, N501Y, and D1118H was the signature for the B.1.1.7 (20H/501Y.V1) variant.
Result: Genetic variability of the SARS-CoV-2 and number of spike mutation containing variants increased rapidly since September 2020, with additional accumulation of P681H, N439
Discussion: In this period, we report an expansion of more virulent variants such as B.1.1.7 VOC and strains not associated with the increased transmissibility per se but containing the described above DeltaH69_V70, N439K, or P681H mutations.
Detection and characterization of the SARS-CoV-2 lineage B.1.526 in New York.
SARS_CoV_2 mutation literature information.
PMID: 
2021
Nature communications
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