Method: The mutations (Delta69/70, Delta144, N501Y, A570D,
Result: In addition to RBD N501Y and NTD DeltaH69/V70, B.1.1.7 is defined by further S mutations across S2 (T716I, S982A, and D1118H) and S1 (DeltaY144, A570D, and P681H) (Figure 6A).
Discussion: In addition, a recent report on the S1/S2 cleavage site mutation P681H demonstrated that enhanced cleavage of spike P681H was not associated with increased PV infectivity or cell fusion relative to the WT.
Analysis of SARS-CoV-2 variant mutations reveals neutralization escape mechanisms and the ability to use ACE2 receptors from additional species.
Introduction: A few mutations in the SD1-2 region near the Furin cleavage site are also identified, such as P681H in B.1.1.7 and A701V in B.1.351.
Method: The variant B.1.1.7 (GISAID: EPI_ISL_601443) was constructed with total of 9 mutations including 69-70del, 144del, N501Y, A570D, D614G, P681H, T716I, S982A and D1118H.
Specific allelic discrimination of N501Y and other SARS-CoV-2 mutations by ddPCR detects B.1.1.7 lineage in Washington State.
Result: For each sample, a total of nine mutations were found in the spike protein: H69-70-, Y144-, N501Y, A570D, D614G, P681H, T716I, S982A, D1118H.
Discussion: The large number of genomic mutations associated with B.1.1.7, including SNPs (A570D, D614G, P681H, T716I, S982A, and D1118H) highlight the need to rapidly distinguish these subtle mutations in emerging VOCs.
Ultrapotent miniproteins targeting the SARS-CoV-2 receptor-binding domain protect against infection and disease.
Result: Accordingly, we evaluated the activity of LCB1v1.3 against a B.1.1.7 isolate containing deletions at 69-70 and 144-145 and substitutions at N501Y, A570D, D614G, and P681H, and against a recombinant WA1/2020 strain containing key substitutions present in the B.1.351 and B.1.1.28 variant strains at residues E484K, N501Y, and D614G.
Occurrence of a novel cleavage site for cathepsin G adjacent to the polybasic sequence within the proteolytically sensitive activation loop of the SARS-CoV-2 Omicron variant: The amino acid substitution N679K and P681H of the spike protein.
Result: As the P681H mutation is also found in the B.1.1.7 variant, its frequency has increased in some locations since late December 2020, following the first introduction of B.1.1.7 into Israel.
Result: Characterization of the B.1.1.50 + P681H Variant.
Result: Effective Neutralization of the B.1.1.50 + P681H Variant.
Result: Identification of the P681H Mutation in Sewage.
Result: The B.1.1.50 + P681H variant is characterized by the non-synonymous S protein mutation P681H (C23604A) and four additional synonymous mutations: Nsp3:C7765T, Nsp12b:C13821T, Nsp16: PMID: 34204754
2021
Viruses
Introduction: The major changes are the mutation N501Y in the receptor-binding domain (RBD); the deletion 69-70 which may increase transmissibility and produces a false negative in certain RT-PCR-based diagnostic assays; and the mutation P681H, next to the furin cleavage site, that could impact antigenicity and enhance viral infectivity.
Immune Evasion of SARS-CoV-2 Emerging Variants: What Have We Learnt So Far?
Introduction: The P681H change is adjacent to the furin cleavage site and could potentially have an effect on S1/S2 cleavage and therefore on cell entry and infectivity.
Introduction: The first variant Alpha or B.1.1.7 that raised global concerns about increased transmissibility and potential immune evasion harbors seven missense mutations (N501Y, A570D, D614G, P681H, T716I, S982A, D1118H) and three deletions in spike (69/70del and 144del) (Figure 3).
Anti-SARS-CoV-2 Vaccines and Monoclonal Antibodies Facing Viral Variants.
Introduction: It carries Spike mutations including E484K, N501Y, D614G, P681H/R, and V1176F.
Introduction: It carries the N501Y mutation in the receptor-binding domain (RBD), and other mutations including the 69/70 deletion, which lead to a conformational change in the Spike protein and the P681H near the site of S1/S2 furin cleavage.
Introduction: It is characterized by 17 non-synonymous mutations, including at least 3 of the 8 present in Spike that have a biological importance: (1) N501Y, which has a role in enhancing the binding affinity to ACE2R; (2) the
A Comprehensive Molecular Epidemiological Analysis of SARS-CoV-2 Infection in Cyprus from April 2020 to January 2021: Evidence of a Highly Polyphyletic and Evolving Epidemic.
Abstract: Genetic analysis of whole SARS-CoV-2 genomic sequences of the aforementioned lineages revealed the presence of mutations within the S protein (L18F, DeltaH69/V70, S898F, DeltaY144, S162G, A222V, N439K, N501Y, A570D, D614G, P681H, S982A and D1118H) that confer higher transmissibility and/or antibody escape (immune evasion) upon the virus.
Introduction: Furthermore, mutations/deletions in the S-protein, such as L18F, DeltaH69/V70,
Introduction: VOC, 202012/01) with multiple S protein mutations (deletion 69-70, deletion 144, N501Y, A570D, D614G, P681H, T716I, S982A, D1118H) spread rapidly across South East England and London.
SARS_CoV_2 mutation literature information.
PMID: 
2021
Cell reports
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