Precision Response to the Rise of the SARS-CoV-2 B.1.1.7 Variant of Concern by Combining Novel PCR Assays and Genome Sequencing for Rapid Variant Detection and Surveillance.
Introduction: For example, B.1.1.7 is characterized by a number of mutations in the S gene, including the H69/V70 and Y144 deletions, N501Y, and P681H, among a number of other changes.
Characterization of the emerging B.1.621 variant of interest of SARS-CoV-2.
PMID: 34403832
2021
Infection, genetics and evolution
Abstract: This strategy allowed us to identify the emergence of the B.1.621 lineage, considered a variant of interest (VOI) with the accumulation of several substitutions affecting the Spike protein, including the amino acid changes I95I, Y144T, Y145S and the insertion 146 N in the
Introduction: In this study, we reported the emergence and spread of the novel B.1.621 lineage of SARS-CoV-2, a new VOI with the insertion 146 N and several amino acid substitutions in the Spike protein (Y144T, Y145S, R346K, E484K, N501Y and P681H).
The Emergence and Spread of Novel SARS-CoV-2 Variants.
Result: It shares mutation P681H with B.1.1.7, which may represent an independent homogeneity of the UK strain.
Result: Third, mutation P681H is located near the insertion sites of four amino acids, connecting S1 and S2 subunits in S protein, in other words, adjacent to the furin cleavage site, which may cause S protein to be more easily cleaved by the protease, thereby, enhancing its affinity with the ACE2 receptor and promoting the virus to enter respiratory epithelial cells.
Table: P681H
Emergence of the First Strains of SARS-CoV-2 Lineage B.1.1.7 in Romania: Genomic Analysis.
Result: Furthermore, the P681H mutation of the S protein might influence the cleavage of the S protein due to its proximity to the S1/S2 furin cleavage site.
Epitope diversity of SARS-CoV-2 hyperimmune intravenous human immunoglobulins and neutralization of variants of concern.
Abstract: However, the key mutations-N501Y (44.6%), S982A (44.4%),
Result: N501Y and P681H are key mutations in the alpha variant.
Result: The most common amino acid substitutions were D614G (83.4%) followed by N501Y (44.6%), S982A (44.4%), A570D (43.3%), T716I (40.4%), and P681H (40.1%) in Spike (S) protein.
Discussion: Other important mutations, N501Y and P681H, specific for the emerging alpha variant of concern were found at a high percentage among the environmental strains.
Novel Nested-Seq Approach for SARS-CoV-2 Real-Time Epidemiology and In-Depth Mutational Profiling in Wastewater.
PMID: 34445204
2021
International journal of molecular sciences
Result: According to the % frequencies of the genetic markers A570D (23271C>A), D614G (23403A>G), P681H (23604C>A), T716I (23709C>T), S982A (24506T>G), and D1118H (24914GG>C), the Beta.1.1.7/alpha lineage VOC was detected in 80.6% +- 8.3 (mean +- SE), (median of 80.8%) of the total sequencing reads.
Emergence and spread of the potential variant of interest (VOI) B.1.1.519 of SARS-CoV-2 predominantly present in Mexico.
Abstract: In the present work, we report the identification of a potential variant of interest, harboring the mutations T478K, P681H, and T732A in the spike protein, within the newly named lineage B.1.1.519, that rapidly outcompeted the preexisting variants in Mexico and has been the dominant virus in the country during the first trimester of 2021.
Introduction: A phylogenomic analysis of genomic sequences using the Nextstrain tool showed that the viruses in the lineage B.1.1.519 (B.1.1.1.222+T478K+P681H+T732A) group independently of the lineage B.1.1.222 sequences, strongly suggesting that this variant should be classified as a variant of interest (VOI).
Introduction: Finally, two variants with interesting features were identified in this
Predominance of the SARS-CoV-2 Lineage P.1 and Its Sublineage P.1.2 in Patients from the Metropolitan Region of Porto Alegre, Southern Brazil in March 2021.
SARS_CoV_2 mutation literature information.
PMID: 
2021
Microbiology spectrum
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