Result: Result: P681H displayed the greatest fusogenicity of all investigated mutations, being almost 2.5-fold higher than D614G S (Figs 4A and EV4E).
Result: Alpha S contains the 69/70 and Y144 deletions in the N-terminal domain (NTD), P681H and T716I mutations in the S1/S2 cleavage site, the S982A mutation in the heptad repeat 1 (HR1) site and the D1118H mutation in between HR1 and HR2.
Result: Of the mutations that are associated with Alpha, we found that the 69/70 deletion in the NTD decreased cell-cell fusion whereas P681H and D1118H substitutions both increase fusion (Figs 4A and EV4E).
Rapid and High-Throughput Reverse Transcriptase Quantitative PCR (RT-qPCR) Assay for Identification and Differentiation between SARS-CoV-2 Variants B.1.1.7 and B.1.351.
Discussion: SC-2 variant B.1.1.7 contains numerous synonymous and nonsynonymous mutations, of which the spike gene mutations 69-70del, N501Y, and P681H received most attention due to their potential effect on virus infectivity.
Distant residues modulate conformational opening in SARS-CoV-2 spike protein.
Result: The B.1.1.7 also shows a P681H mutation close to the highly correlated N679 residue predicted from our model (SI Appendix.
Evaluation of the clinical and analytical performance of the Seegene allplex SARS-CoV-2 variants I assay for the detection of variants of concern (VOC) and variants of interests (VOI).
Introduction: In September 2020, the B.1.1.7 lineage emerged as a variant of concern in the United Kingdom (UK), subsequently termed the alpha variant, with 9 spike protein mutations (del69/70HV, del144Y, N501Y, A570D, D614G, P681H<
Table: P681H
Discussion: Seegene has also launched the AllplexSARS-CoV-2 Master assay that can detect the presence of SARS-CoV-2 via the detection of E, N and RdRP genes and the presence of S gene mutations such as HV69/70 deletion, Y144 deletion, E484K, N501Y and P681H.
SARS-CoV-2 monoclonal antibodies with therapeutic potential: Broad neutralizing activity and No evidence of antibody-dependent enhancement.
Figure: (a) Binding responses of anti-SARS-CoV-2 S protein mAbs, S1D2-hIgG1, STI-1499-LALA and 1741-LALA to the S1 fragment of the S protein from SARS-CoV-2 variants, including 2019-nCoV, B.1.1.7 (HV69-70 deletion, Y144 deletion, N501Y, A570D, D614G, P681H)-, B.1.351 (K417N, E484K, N501Y, D614G)-, and B.1.617.2 (T19R, G142D, E156G, 157-158 deletion, L452R, T478K, D614G, P681R)- lineage.
Ten emerging SARS-CoV-2 spike variants exhibit variable infectivity, animal tropism, and antibody neutralization.
Discussion: The increased affinity to ACE2 receptors caused by RBD mutations (e.g., E484K, N501Y) or increased cleavage activity by mutations adjusted to the furin site (e.g., P681H) may facilitate the function of enzymes.
Genomic reconstruction of the SARS-CoV-2 epidemic in England.
Introduction: In contrast to other VOCs, Delta/Kappa do not contain N501Y or E484K mutations, but their L452R mutation may reduce antibody recognition and P681R enhances furin cleavage, similar to the P681H mutation of Alpha.
Introduction: The growth advantage is thought to stem, at least in part, from spike mutations that facilitate ACE2 receptor binding (N501Y) and furin cleavage (P681H).
The evaluation of potential global impact of the N501Y mutation in SARS-COV-2 positive patients.
Introduction: P681H is adjacent to the furin cleavage site, a location known to be of biological importance.
Introduction: Three mutations in the S protein of the novel variant (N501Y, HV69-70del, and P681H) have potential biological implications.
Result: List of variations displayed in structure (nearest residue if in loop/termini region): H69del V70del(69) Y144del(143) N501Y A570D D614G P681H(674) T716I&n
Discussion: Three mutations in the S protein of the novel variant (N501Y, HV69-70del, and P681H) have potential biological implications.
Rapid Automated Screening for SARS-CoV-2 B.1.617 Lineage Variants (Delta/Kappa) through a Versatile Toolset of qPCR-Based SNP Detection.
Abstract: METHODS: We created a broad set of single nucleotide polymorphism (SNP)-assays including del-Y144/145, E484K, E484Q, P681H
Introduction: Spike gene mutations such as N501Y, E484K/Q, L452R, and P681H/R have occurred independently in multiple VOCs and VOIs (variants of interest), enabling dynamic adaptation of existing assay designs to detect emerging SARS-CoV-2 variants.
Discussion: For example, the recently emerged B.1.617 (first detected in India) lineages feature E484Q (only B.1.617.1/3) and P681R SNPs instead of the more common E484K and P681H.
Biological Significance of the Genomic Variation and Structural Dynamics of SARS-CoV-2 B.1.617.
Introduction: L452R is located in the RBD, and P681H or P681R is located in the furin cleavage site.
Introduction: Some of the aa changes or similar ones in B.1.617 were also identified in other circulating lineages: D614G was also found in B.1 lineage, L452R in B.1.526 (Iota), and P681H in B.1.1.7 (Alpha).
SARS_CoV_2 mutation literature information.
PMID: 
2021
The EMBO journal
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