Result: All these analyses showed that the mutant A97V-RdRp does not have a large effect on RDV binding, in contrast, P323L-RdRp structure has a stabilising effect on RDV binding.
Result: As for the P323L-apo (pink) structure, the RMSD showed a similar profile to the WT until 175 ns, where the structure decreased instability and the RMSD increased to 3.5 A.
Result: As such, the eigenvectors showed that the P323L-RdRp that bound to RDV contributed to a 13% variance in comparison to WT-RdRp and A97V-Rdrp (Figure 6C).
Result: As such, these results suggested that P323L induced a confo
Concurrent mutations in RNA-dependent RNA polymerase and spike protein emerged as the epidemiologically most successful SARS-CoV-2 variant.
Figure: Temporal and spatial distribution of the four possible D614G and P323L combinations.
Figure: Temporal evolution of the four possible D614G and P323L variants in different countries.
Figure: Two major protein variants of SARS-CoV-2: S protein D614G and RNA-dependent polymerase P323L.
Discussion: Although more experiments are needed to understand the underlying biological relevance of the two mutations, we speculate that the P323L mutation attenuates the clinical presentation of the SARS-CoV-2.
Discussion: Firstly, based on its location, we expect the P323L mutation to negatively impact the nsp8 association.|
High-precision and cost-efficient sequencing for real-time COVID-19 surveillance.
Result: 2a) had three nucleotide substitutions from the reference sequence, Wuhan-Hu-1, and two amino acid changes, P323L in the RNA-dependent RNA polymerase (RdRP, NSP12) and D614G in the S protein.
Result: Mass circulation of G clade strains were confirmed by around 80% prevalence of P323L in RdRP and D614G in the S protein globally.
Table: P323L
Figure: All 25 sequences were classified as G clade with mutations P323L in NSP12 (RdRP) and D61
Genomic characterization of SARS-CoV-2 isolates from patients in Turkey reveals the presence of novel mutations in spike and nsp12 proteins.
Abstract: Besides, mutations in SR-rich region of N protein and P323L in RDRP were also present.
Discussion: In this study, signature nonsynonymous mutations leading to amino acid changes of P323L in the RdRp was found (Supplementary Table ST2), which is involved in the replication of the viral genome.
Discussion: Notably, few other previous studies although suggest the involvement of the diseases severity with the specific mutation (D614, P323L) in the SARS-CoV-2 genome.
Discussion: On the other hand, the temporal analysis of mutation accumulation also showed mutations (P323L, I120F, D614G, R203K,
No association between the SARS-CoV-2 variants and mortality rates in the Eastern Mediterranean Region.
Abstract: Two substitutions, spike_D614G and NSP12_P323L, were predominantly concurrent in most countries.
Abstract: While the single incidence of NSP12_P323L was positively correlated with higher case fatality rates in EMR, no such association was established for the double (spike_D614G and NSP12_P323L) concurrent variant across the region.
Evolutionary Tracking of SARS-CoV-2 Genetic Variants Highlights an Intricate Balance of Stabilizing and Destabilizing Mutations.
Result: Notably, founder lineage B.1 and its sublineages B.1.X, B.1.1.X, D.X, and C.X that carry both D614G and P323L mutations have become the dominant variants across the world (87% of global collection per CoV-GLUE as of 30 November 2020).
Result: The predominant lineage-defining mutations in the whole data set were D614G (85.5%) and P323L (85.5%), after originally appearing in late January 2020.
Result: Two mutations have become consensus: D614G in S (nucleotide 23,403, A to G) and P323L (also known as P4715L) in nsp12 (nucleotide 14,143, C to T).
Table: P323L
Introduction and Characteristics of SARS-CoV-2 in North-East of Romania During the First COVID-19 Outbreak.
Discussion: As a consequence, a synonymous mutation in NSP3, a P323L mutation in RNA primase, and a mutation in spike glycoprotein (614 D > G) occurred.
Conformational Variability Correlation Prediction of Transmissibility and Neutralization Escape Ability for Multiple Mutation SARS-CoV-2 Strains using SSSCPreds.
Introduction: After March 2020, only two mutations, RNA-directed RNA polymerase P323L (ORF1ab P4715L, ORF1b P314L) and spike protein D614G had nearly overwhelmed the original mutation sites ().
Correlates of SARS-CoV-2 Variants on Deaths, Case Incidence and Case Fatality Ratio among the Continents for the Period of 1 December 2020 to 15 March 2021.
SARS_CoV_2 mutation literature information.
PMID: 
2021
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