Discussion: About 65.6% of the viruses belong to clades 20A, 20B, and 20C and non-synonymous mutations leading to two amino acid substitutions, the D614G in the spike and P323L in nsp12, were predominant in most countries.
Discussion: It should be noted, the P323L substitution has been frequently observed in nsp12 in the strain spreading in Africa.
Genomic analysis of early transmissibility assessment of the D614G mutant strain of SARS-CoV-2 in travelers returning to Taiwan from the United States of America.
Result: In addition to the S-protein D614G mutation, other missense mutations were identified in NSP12 (RNA dependent RNA polymerase; RdRp) with a P323L mutation, a Table: P323L
Discussion: Our current findings are consistent with this observation, in that the viral genome sequences of the SARS-CoV-2 strains from Cases 1-4 harbored both the D614G and P323L mutations.
Discussion: Previous studies have shown that the P323L mutation in NSP12 co-evolved with the D614G mutation in S-protein.
SARS-CoV-2 promotes RIPK1 activation to facilitate viral propagation.
Result: To determine the functional significance of the differential interaction mediated by the original variant NSP12 323P and NSP12 323L encoded by the C14408T variant with RIPK1, we isolated a virus strain carrying the C14408T (NSP12 P323L) mutation from a patient nasopharyngeal swab.
Discussion: Furthermore, the increased ability of SARS-CoV-2 323L variant to stimulate RIPK1 activation and replicate in the lung organoids also suggests that the NSP12 P323L mutation may contribute to the spread of this SARS-CoV-2 variant around the world.
Discussion: In addition, since the structural changes in NSP12 introduced by
The evaluation of potential global impact of the N501Y mutation in SARS-COV-2 positive patients.
Result: P323L mutation of RdRp may change binding affinity to the Ubl1 region of NSP3 (Figure 1).
Result: The S:p.D614G might assist in rapid entry into the host cells followed by quick dissemination, and the RdRp:p:P323L may instead boost the replication by a faster RNA processing (exiting).
Result: Therefore, the P323L mutation within the RdRp interface domain's conserved site may only affect the RdRp-NSP8 interaction without changing metal binding affinity.
Table: P323L
Analysis of 329,942 SARS-CoV-2 records retrieved from GISAID database.
PMID: 34735950
2021
Computers in biology and medicine
Discussion: For example, D614G is often considered together with P323L.
Changing predominant SARS-CoV-2 lineages drives successive COVID-19 waves in Malaysia, February 2020 to March 2021.
Result: Nonsynonymous mutations seen in the B.1.524 lineage sequences were nsp3-T1198I, nsp4-T28I, nsp5-T24A, nsp12-P323L, nsp13-L428F, spike-D614G, spike-A701V, and N-S194L.
Discussion: The nsp12 (RNA-dependent RNA polymerase) P323L mutation present in lineage B.1.524 is almost always associated with PMID: 34764315
2021
Scientific reports
Result: The C14408T was encoded for substitution of proline with leucine at position 323 in nsp12 of ORF1ab (nsp12-P323L).
Table: P323L
An outbreak of SARS-CoV-2 with high mortality in mink (Neovison vison) on multiple Utah farms.
Result: One of the two mink from farm A had mutations at T85I-NSP2, S1205L-NSP3, Table: P323L
Discussion: Success of the P323L/ G614 variant suggests that the P323L mutation adds to the virulence of the G614 spike variant, although without increasing patient mortality.
Discussion: The P323L-NSP12 mutation in the viral polymerase gene coevolved with the D614G-spike mutation also present in this mink strain to become the most prevalent variant in the world, and this combination is present in 95.4% of whole virus genomes from mink to date.
Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) in a Dog in Connecticut in February 2021.
Abstract: It contained both the D614G in spike and P323L in nsp12 substitutions, which have become the dominant mutations in the United States.
Result: The D614G and P323L occurred in China on 24 January 2020 and in the U.K.
Result: The B.1 and its sub-lineages that carry both D614G in spike and P323L in nsp12 substitutions have become the dominant variants across the world.
Result: We found amino acid substitutions in Spike (D614G), N (D377Y, P67S
SARS_CoV_2 mutation literature information.
PMID: 
2021
Frontiers in medicine
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