Introduction: The non-structural proteins encoded by the ORF1ab contain mutations in the nsp3 (K38R, V1069I, Delta1265, L1266I, A1892T), nsp4 (T492I), nsp5 (P132H), nsp6 (Delta105-107, A189V), nsp12 (P323L), and nsp14 (I42V).
Importation, circulation, and emergence of variants of SARS-CoV-2 in the South Indian state of Karnataka.
Discussion: The lineage is characterized by the following amino acid replacements- nsp12-P323L(95.38%), S-D614G (93.85%), S-N440K (56.92%), ORF 3a-Q57H (90.77%), ORF 3a-E261*(81.54%), nsp3-T183I (81.54%), nsp16-L126F(80%), N-S2P (72.31%), ORF 8-S97I (72.31%) (extended data, Supplementary Table 7 ).
Mutations in SARS-CoV-2 viral RNA identified in Eastern India: Possible implications for the ongoing outbreak in India and impact on viral structure and host susceptibility.
Result: These consisted of the 14408 C/T mutation resulting in a change of P323L in the RdRp and
Table: P323L
Discussion: In particular, the P323L was present in all A2a sequences in our samples.
Discussion: Sequence obtained from one of the samples S10, which belonged to the clade B4, did not possess the P323L mutation.
Discussion: The A2a clade is characterized by the signature nonsynonymous mutations leading to amino acid changes of P323L in the RdRp which is involved in replication of the viral genome and the change of D614G in the Spike glycoprotein which is essential for the entry of the virus in the host cell by binding to the ACE2 receptor.
An updated analysis of variations in SARS-CoV-2 genome.
Discussion: C14408T mutation within the RNA-dependent RNA polymerase encoding region of ORF1b, which is a missense mutation that leads to an amino acid change from proline to leucine at position 323 (P323L) in RNA polymerase protein, was amongst the most commonly seenmutations [61% (38/62)] in isolates from Turkey.
Tracking Changes in SARS-CoV-2 Spike: Evidence that D614G Increases Infectivity of the COVID-19 Virus.
5Result: At that time, the G614 form was rare globally but gaining prominence in Europe, and GISAID was also tracking the clade carrying the D614G substitution, designating it the ""G clade."" The
Figure: Of note, the TTCG form did not expand, and it lacked the RdRp P323L change, raising the possibility that the P323L change may contribute to a selective advantage of the haplotype.
Figure: One is in the RdRp protein (nucleotide C14408T resulting in a P323L amino acid change), one in Spike (nucleotide A23403G resulting in the D614G amino acid change) and one is silent (C3037T).
Variant analysis of SARS-CoV-2 genomes.
PMID: 32742035
2020
Bulletin of the World Health Organization
Result: The most common variants were the synonymous variant 3037C > T (6334 samples), ORF1ab P4715L (RdRp P323L; 6319 samples) and SD614G (6294 samples).
Result: We also detected mutations in the nonstructural protein RNA-dependent RNA polymerase (RdRp), such as P323L (6319 samples).
Discussion: Almost all strains with D614G mutation also have a mutation in the protein responsible for replication (ORF1ab P4715L; RdRp P323L), which might affect replication speed of the virus.
RdRp mutations are associated with SARS-CoV-2 genome evolution.
Introduction: The proline to leucine substitution (P323L) caused by the 14408C>T mutation has been suggested to rigidify the RdRp protein structure, which may exert its effects through altered interaction with other components of the replication/transcription machinery or with the RNA template, thereby resulting in an altered mutation rate.
Result: Although preliminary studies suggest that 14408C>T (P323L in RdRp) could lower replication fidelity, it is less clear how the synonymous 15324C>T mutation could lead to lower mutation rates.
Characterizing SARS-CoV-2 mutations in the United States.
Introduction: Based on genotyping results, top eight missense mutations (i.e., 14408C>T-(P323L), 23403A>G-(D614G), 25563G>T-(Q57H), 1059C>T-(T85I), 28144T>C-(L84S), 17858A>G-(Y541C), 17747C>T-(P504L), and 27964C>T-(S24L)) are identified, in addition to three synonymous mutations (i.e., 3037C>T-(F106F), 8782C>T-(
Molecular Epidemiology Analysis of SARS-CoV-2 Strains Circulating in Romania during the First Months of the Pandemic.
Result: These mutations, C3037T, C14408T (P323L in Nsp12) and A23403G (D614G in S), were present in all Romanian sequences.
Result: When looking into the GISAID database mutational statistics, which uses hCoV-19/Wuhan/WIV04/2019 EPI_ISL_402124 as a reference strain (COVsurver tool), both P323L and D614G mutations are present in a high percentage (61.7%/61.8%, n = 33480 and n = 33537).
SARS_CoV_2 mutation literature information.
PMID: 
2021
Frontiers in immunology
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