Genomic characterization unravelling the causative role of SARS-CoV-2 Delta variant of lineage B.1.617.2 in 2nd wave of COVID-19 pandemic in Chhattisgarh, India.
Result: Notably, all variants showed P323L mutation in nonstructural protein (NSP) 12 encoding RNA dependent RNA polymerase (RdRP) gene, and Delta variant exclusively showed I82T mutation in membrane protein-encoding gene.
Table: P323L
Discussion: P323L leads to stability of the tertiary protein structure of the virus to help in virus successful survival in different geographical conditions.
Discussion: All lineages have shown P323L mutation in the interface domain of the RdRP region.
High diversity in Delta variant across countries revealed by genome-wide analysis of SARS-CoV-2 beyond the Spike protein.
Result: Strikingly, all these mutations except P323L in NSP12 are nearly exclusive to the Delta variant compared to other variants of concern (Alpha, Beta, and Gamma variants of SARS-CoV-2) (mean prevalenceDelta = 99.74%, mean prevalenceotherVariantsofConcern = 0.12%) (Fig EV2, Appendix Table S1).
Result: We identified seven highly prevalent mutations in the following proteins of the Delta variant: Membrane (I82T: 99.9%), Nucleocapsid (R203 M: 99.9%, D377Y: 99.6%), NSP12 (P323L: 99.9%), NS3 (S26L: 99.9%), and NS7a (V82A: 99.4%, T120I<
Versatile SARS-CoV-2 Reverse-Genetics Systems for the Study of Antiviral Resistance and Replication.
Abstract: We found that putative coronavirus remdesivir resistance-associated substitutions F480L and V570L-and naturally found polymorphisms A97V, P323L, and N491S, all in nsp12-did not decrease SARS-CoV-2 susceptibility to remdesivir.
Result: Among natural nsp12 polymorphisms, we investigated the P323L mutation, known as the major polymorphic site of nsp12 (>90% prevalence found in GISAID EpiCov database accessed 10 May 2021).
Variants of SARS CoV-2: mutations, transmissibility, virulence, drug resistance, and antibody/vaccine sensitivity.
PMID: 35227008
2022
Frontiers in bioscience (Landmark edition)
Abstract: Mutations in the S protein that are common among several of the variants include D614G that increases transmissibility and viral load and is often associated with P323L on the RNA dependent RNA polymerase.
The dynamics of circulating SARS-CoV-2 lineages in Bogor and surrounding areas reflect variant shifting during the first and second waves of COVID-19 in Indonesia.
Result: Notably, a total 199 out of 202 viruses (99.9%) that we sequenced carried the S_D614G and NSP12_P323L non-synonymous mutations.
Figure: One virus (hCoV-19/Indonesia/JK-LIPI135/2021) not shown in this figure was found to carry multiple amino acid changes, including S_D614G, S_P681R, S_T478K, S_L452R, NSP6_T77A, NSP3_P1228L, NSP12_P323L, NSP12_G671S, and NSP14_A394V.
Figure: The highest incidence of sub
Identification of a novel SARS-CoV-2 variant with a truncated protein in ORF8 gene by next generation sequencing.
Role of the Microbiome in the Pathogenesis of COVID-19.
PMID: 35433495
2022
Frontiers in cellular and infection microbiology
Introduction: These had two co-evolving NS mutants, which differ in RdRp (P323L) and spike (S) protein (D614G).
Prolonged shedding of infectious viruses with haplotype switches of SARS-CoV-2 in an immunocompromised patient.
PMID: 35430092
2022
Journal of infection and chemotherapy
Discussion: P314L (P323L) had been one of the common mutations in PANGON lineage B, and all seven haplotypes in our study possessed the mutation as the background.
Immune escape facilitation by mutations of epitope residues in RdRp of SARS-CoV-2.
PMID: 35293850
2022
Journal of biomolecular structure & dynamics
Abstract: The mutation P323L in RdRp is associated with the loss of a particular epitope (321-327) from this protein.
Abstract: We consider the effects of mutations in some of the epitope region including the naturally occurring mutation P323L on the structure of the epitope and their interface with paratope using all-atom molecular dynamics (MD) simulation studies.
Heat Treatment Promotes Ubiquitin-Mediated Proteolysis of SARS-CoV-2 RNA Polymerase and Decreases Viral Load.
Abstract: A mild daily heat treatment maintains low levels of both wild-type and P323L mutant of NSP12, suggesting clinical potential.
Abstract: Here, we reveal a potential mechanism by which mild heat treatment destabilizes the wild-type RNA-dependent RNA polymerase (also known as nonstructural protein 12 (NSP12)) of SARS-CoV-2 as well as the P323L mutant commonly found in SARS-CoV-2 variants, including omicron and IHU.
Intr
Introduction: Although our study lacks adequate evidence obtained from in vivo animal model showing the inhibitory effect of mild heat treatment on the SARS-CoV-2 virulence, we do provide clear evidence that mild daily treatment is sufficient to maintain low levels of both WT and P323L mutant of NSP12.
SARS_CoV_2 mutation literature information.
PMID: 
2022
Microbial pathogenesis
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