Result: Sequences from this study also share four nonsynonymous mutations, P314L (Nsp12), D614G (Spike), R203K and G204R (Nucleocapsid), with these placenta sequences, in addition to two synonymous mutations (214C>T, 3037 C>T).
Discussion: Mutation P314L in NSP12 (RdRp) has also been linked to increased viral fitness.
Discussion: The presence of both aa changes, D614G in S protein and P314L in NSP12 has been asso
High throughput sequencing based direct detection of SARS-CoV-2 fragments in wastewater of Pune, West India.
PMID: 34688738
2022
The Science of the total environment
Table: P314L
Cross-sectional genomic perspective of epidemic waves of SARS-CoV-2: A pan India study.
Abstract: Nation-wide mutational analysis depicted >0.5 million mutation events with four major mutations in >19,300 genomes, including two mutations in coding (spike (D614G), and NSP 12b (P314L) of rdrp), one silent mutation (NSP3 F106F) and one extragenic mutation (5' UTR 241).
Result: Two mutations in the protein coding region i.e., D614G in spike and P314L in NSP 12b; one extragenic mutation at 241 position of 5'UTR and one silent mutation at F106 NSP3.
Genetic variations from successive whole genome sequencing during COVID-19 treatment in five individuals.
PMID: 35035981
2022
New microbes and new infections
Abstract: Gene sequencing showed mutation within ORF1b at position P314L.
Introduction: Analysis is also showing mutation within ORF1b at position P314L (P323L) in the initial swab sample in four out of five individuals.
Introduction: Fourth patient, we found substitution at ORF1b:P314L on the second swab.
Introduction: Last patient, we found substitutions at N:D3Q and ORF1b:P314L on the first swab and reversion of both substitutions with addition substitution at N:R203K on the second swab.
Table: P314L
Whole-Genome Sequencing of SARS-CoV-2 Strains from Asymptomatic Individuals in India.
Abstract: All the P.1 sequences included other important mutations, such as P314L and R203K/G204R, and revealed a high genetic diversity in the phylogenetic tree.
Result: In addition, five P.1 nondefining mutations were found in all of the P.1 sequences: two in the ORF1ab (C3037T and P314L) and three in the Nucleocapsid (A28877T, G28878C and R203K/G204R).
Table: P314L
Discussion: Moreover, all the P.1 sequences accumulated other important mutations, such as P314L in the ORF1ab
Genomic Diversity of SARS-CoV-2 in Algeria and North African Countries: What We Know So Far and What We Expect?
Abstract: The effect of mutations in SAR-CoV-2 genomes highlighted similar profiles with D614G spike (S) and ORF1b-P314L variants as the most changes found in 95.3% and 87.9% of total sequences, respectively.
Result: A similar occurrence was also detected for the nucleotide cytosine in position (3037) (C3037T) affecting 1433 (87.91%) genomes causing an amino acid changing mutation in P314L, affecting the NSP12 (non-structural protein 12) and the viral RNA-dependent RNA polymerase (Figure 6 and Figure 7).
Result: Finally, the seven SARS-CoV-2 isolates carried a variety of single nucleotide polymorphisms (SNP) relative to the reference sequence (SARS-CoV-2 isolate Wuhan-Hu-1 NC_045512), including a total of 27 non-synonymous mutations across the genome in different non-structural proteins (nsp), mainly in the RdRp (D54Y and P314L), the papain-like proteinase (nsp3, S126L and T1335I), and nucleocapsid (D22G, S183Y, and D377G) genes (Figure 5).
SARS-CoV-2 Mutations and Their Impact on Diagnostics, Therapeutics and Vaccines.
Result: the analyses showed that B.1 probably originated in China and spread to several European countries reaching Italy several times, forming a large cluster which included initially 59 (around the first week of March) and finally 198 genomes, and 6 further independent introductions mainly corresponding to a group of genomes characterized only by the substitution D614G but lacking other substitutions, in particular the P314L in the RdRp identifying the clade 20A (lineage B.1, clade 19A).
Table: P314L
Discussion: The second phylogeographic scenario involving lineage B.1, showed initially only a few introductions from Asia to Italy and Europe (more precisely defined as Germany in the more detailed country based analysis), of small clusters corresponding to ancestral B.1/19A isolates, characterized by the substitution
ViMRT
SARS_CoV_2 mutation literature information.
PMID: 
2022
Microbes and infection
