Result: Given these results with viruses encoding E484K mutations, we performed separate studies with human convalescent serum (n =10) and a chimeric SARS-CoV-2 WA1/2020 strain encoding a Brazilian variant spike gene (Wash BR-B.1.1.248; L18F, T20N, P26S, D138Y, R190S, K417T, E484K, N501Y, D614G, H655Y, T1027I, and V1176F [Extended Data Fig 5a]).
Genomics and epidemiology of a novel SARS-CoV-2 lineage in Manaus, Brazil.
Result: Although P.1 does not harbor NTD deletions, the changes L18F, T20N, and P26S would be expected to impact markedly on binding at the NTD epitope.
Result: Although P.1 does not harbor deletions in the NTD like B.1.1.7 (Delta69-70,Delta144) or B.1.351 (Delta242-244), it is clear that the NTD mutations in P.1 (L18F, T20N, P26S, D138Y, and R190S) disrupt the epitope for mAb159 (Figure 4A).
Result: Compared to the Wuhan sequence, P.1 contains the following mutations: L18F, T20N, P26S, D138Y, and R190S in the NTD; K417T, E484K, and N501Y in the RBD; D614G and H655Y at the C terminus o
Genomics and epidemiology of the P.1 SARS-CoV-2 lineage in Manaus, Brazil.
Introduction: Lineage P.1 contains 10 lineage-defining amino acid mutations in the virus spike protein (L18F, T20N, P26S, D138Y, R190S, K417T, E484K, N501Y, H655Y, and T1027I) compared with its immediate ancestor (B.1.1.28).
A Potential SARS-CoV-2 Variant of Interest (VOI) Harboring Mutation E484K in the Spike Protein Was Identified within Lineage B.1.1.33 Circulating in Brazil.
Introduction: The VOC P.1, first described in January 2021, displayed an unusual number of lineage-defining mutations in the S protein (L18F, T20N, P26S, D138Y, R190S, K417T, E484K, N501Y, H655Y, T1027I) and its emergence was associated with a second COVID-19 epidemic wave in the Amazonas state.
Mutation Signatures and In Silico Docking of Novel SARS-CoV-2 Variants of Concern.
Result: The BR-VOC is highly mutated in the S-gene resulting in 12 aa substitutions in the S-protein including L18F, T20N, P26S, D138Y, R190S, K417T, E484K, N501Y, D614G, H655Y, T1027I, and V1176F.
E484K as an innovative phylogenetic event for viral evolution: Genomic analysis of the E484K spike mutation in SARS-CoV-2 lineages from Brazil.
PMID: 34044192
2021
Infection, genetics and evolution
Result: For these residues under adaptive pressure, six are included in known mutation sites of spike protein, including E484K (L5F, S12F, P26S, D138Y, A688V).
Result: Regarding the lineage-defining mutations from P.1 lineages, 14 of a total of 19 genomes from the Amazonas monophyletic group present the spike mutations L18F, T20N, P26S, D138Y, and R190S.
SARS_CoV_2 mutation literature information.
PMID: 
2021
Virusdisease
Browser Board
Co-occurred Entities
Filtrator
ViMRT