Conclusion: The N501Y mutation leads to a Y484(S) K353(ACE2) contact described by a collection of orbital interactions, second in strength only to the interactions arising because of the E484K replacement.
Conclusion: To summarize, we offer a dissection of the explicit residue to residue intermolecular interactions resulting from the specific S477N, N501Y, K417N, K417T,
Method: N501Y, K417T, E484K: Professor Dong-Qing Wei from the Shanghai Jiao Tong University provided us with the required snapshots from their MD trajectories published in reference [47].
Monitoring the SARS-CoV-2 pandemic: screening algorithm with single nucleotide polymorphism detection for the rapid identification of established and emerging variants.
PMID: 34537361
2022
Clinical microbiology and infection
Result: N501Y.
Result: Combined detection of the N501Y and DeltaH69-V70 mutations had a 100.0% concordance with WGS for detection of the B.1.1.7 lineage in 144 samples and all were SGTF.
Result: Four RNA extracts failed to amplify in the N501Y assay and were not interpretable, sequencing revealed none of these were VOC/VOI, resulting in an OPA of 98.5%.
Result: In all samples of the Beta variant (N=65), the N501Y and E484K mutations were observed in the absence of H655Y and in all strains of the Gamma variant (N=86), the N501Y, E484K and H655Y mutations were detected.
Result: The allocation of the B.1.351 lineage (Beta) and P.1 lineage (Gamma) based o
Assessment of the binding interactions of SARS-CoV-2 spike glycoprotein variants.
PMID: 34545316
2022
Journal of pharmaceutical analysis
Method: SARS-CoV-2 (2019-nCoV) spike S1 rabbit monoclonal antibody (mAb1), SARS-CoV-2 (2019-nCoV) spike neutralizing rabbit monoclonal antibody (mAb2), and recombinant proteins corresponding to the original SARS-CoV-2 spike RBD; original S1; spike S1 variant D614G; and RBD protein variants N501Y, N439K, Y453F, and E84K were purchased from Sino Biological (Wayne, PA, USA).
Result: Aligning with recently reported data, the RBD proteins containing the Y453F and N501Y mutations were found to exhibit si
Proteolytic activation of SARS-CoV-2 spike protein.
Introduction: It does not have mutations found in the aforesaid three VOCs, such as N501Y, E484K, and K417N/T.
Introduction: The alpha-variant emerged in the United Kingdom in November 2020 and has an N501Y mutation that directly affects the interaction with ACE2, making it more contagious than previous strains (Figure 5).
Introduction: The beta and gamma variants emerged in South Africa and Brazil, respectively, and in addition to N501Y, they have mutations, K417N/T and E484K, which may directly affect the interaction with ACE2, resulting in enhanced ACE2 binding (Figure 5).
Emergence in southern France of a new SARS-CoV-2 variant harbouring both N501Y and E484K substitutions in the spike protein.
PMID: 34656702
2022
Journal of virological methods
Abstract: N501Y mutation was successfully detected at an allele frequency as low as 10 % in a sample with mixed SARS-CoV-2 lineage.
Abstract: A total of 31 PANGO lineages were identified from 168 SARS-CoV-2 positive cases, in which 34 samples belonged to N501Y variants, including B.1.1.7 (n = 20), B.1.351 (n = 12) and P.3 (n = 2).
Abstract: In this study, we developed an in-house TaqMan minor groove binder (MGB) probe-based one-step RT-qPCR assay to detect the presence of N501Y mutation in SARS-CoV-2.
Abstract: It enables robust high-throughput screening for surveillance of SARS-CoV-2 variants of concern harbouring N501Y mutation.
Abstract: The analytical sensitivity and the ability of the assay to detect low frequency of N501Y variants were also evaluated.
Abstract: The increasing p
SARS-CoV-2 Causes Lung Infection without Severe Disease in Human ACE2 Knock-In Mice.
Abstract: Because the entry mechanisms of SARS-CoV-2 in mice remain uncertain, we evaluated the impact of the naturally occurring, mouse-adapting N501Y mutation by comparing infection of hACE2 KI, K18-hACE2 Tg, ACE2-deficient, and wild-type C57BL/6 mice.
Abstract: Overall, our study highlights the hACE2 KI mice as a model of mild SARS-CoV-2 infection and disease and clarifies the requirement of the N501Y mutation in mice.
Abstract: The N501Y mutation minimally affected SARS-CoV-2 infection in hACE2 KI mice but was required for viral replication in wild-type C57BL/6 mice in a mACE2-dependent manner and augmented pathogenesis in the K18-hACE2 Tg mice.
Abstract: Thus, the N501Y mutation likely enhances interactions with mACE2 or hACE2 in vivo.
Nucleocapsid mutations in SARS-CoV-2 augment replication and pathogenesis.
Discussion: Overall, in this study we establish that the KR mt enhances SARS-CoV-2 infection relative to WT, increasing viral fitness in vitro and in vivo, which along with the N501Y mutation, likely selected for the emergence of the alpha variant.
A humanized nanobody phage display library yields potent binders of SARS CoV-2 spike.
Abstract: Molecular dynamics simulations provide a structural basis for understanding the neutralization process of nanobodies exclusively focused on the spike-ACE2 interface with and without the N501Y mutation on RBD.
Abstract: The most potent nanobody, RBD-1-2G(NCATS-BL8125), tolerates the N501Y RBD mutation and remains capable of neutralizing the B.1.1.7 (Alpha) variant.
Identification of genotypic variants and its proteomic mutations of Brazilian SARS-CoV-2 isolates.
Discussion: N501Y mutation in the spike protein of B.1.1.7 mutant viruses was experimentally showed to exert enhanced replication rates in cell lines and human respiratory cells (ex-vivo studies), which might occur through the same mechanism (; Moelling, 2021).
Analysis of SARS-COV2 spike protein variants among Iraqi isolates.
Abstract: Twenty-two distinct mutations were identified within the spike protein regions which were: L5F, L18F, T19R, S151T, G181A, A222V, A348S, L452 (Q or M), T478K, N501Y, A520S, A522V, A570D, S605A, D614G, Q675H, N679K, P681H, T716I, S982A, A1020S,
ViMRT
SARS_CoV_2 mutation literature information.
PMID: 
2022
Chembiochem
