literature

SARS_CoV_2 mutation literature information.

Structural and functional insights into the major mutations of SARS-CoV-2 Spike RBD and its interaction with human ACE2 receptor.

PMID: 34955621 2022 Journal of King Saud University. Science

Result: Further, the MM/GBSA binding free energy of the complexes- Native Spike:ACE2, L452R Spike:ACE2, T478K Spike:ACE2 and N501Y

Figure: Schematic diagram of spike protein domains- N-terminal domain (NTD), receptor-binding domain (RBD), fusion peptide (FP), heptapeptide repeat sequence 1 (HR1), heptapeptide repeat sequence 2 (HR2), transmembrane region (TM) and cytoplasm domain (CT), and structural positions of mutations (T478K, L452R and N501Y on spike RBD region.

Isolation of SARS-CoV-2 B.1.1.28.2 (P2) variant and pathogenicity comparison with D614G variant in hamster model.

PMID: 34959053 2022 Journal of infection and public health

Introduction: During January 2021, lineage P.1, also known as Gamma variant, a VOC with 17 amino acid changes which includes N501Y, E484K, and K417N on the spike protein; ORF1b deletion outside the spike protein was detected in the travelers from Brazil at Japan.

Introduction: Furthermore, Brazil reported another variant P.2 lineage, which has E484K mutation but not the N501Y and K417N amino acid changes in the spike protein.

Introduction: Subsequently, another variant B.1.351 (Beta variant) was identified in South Africa with 21 mutations, including N501Y, E484K, and K417

Computational investigation reveals that the mutant strains of SARS-CoV2 have differential structural and binding properties.

PMID: 34968787 2022 Computer methods and programs in biomedicine

Introduction: Another strain in which Asparagine positioned at 501 of RBD is substituted by Tyrosine (N501Y) arose in United Kingdom and South Africa.

Introduction: Of these five, three mutations such as G476S, V483A and N501Y occur at the binding interface of RBD (C-terminal side) that interact with PD (peptidase domain) of the host ACE2 receptor.

Introduction: These mutations (V36

Comparative mutational analysis of SARS-CoV-2 isolates from Pakistan and structural-functional implications using computational modelling and simulation approaches.

PMID: 34968862 2022 Computers in biology and medicine

Result: Recently reported mutations in the spike proteins of SARS-CoV-2 from South Africa (Lys417Asn, Glu484Lys, Asn501Tyr) (501Y.V2Variant) and Brazil (Lys417Asn, Glu484Lys, Asn501Tyr) had led these strains to evade the vaccines successfully.

SARS-CoV-2 wastewater surveillance in Germany: Long-term RT-digital droplet PCR monitoring, suitability of primer/probe combinations and biomarker stability.

PMID: 34968879 2022 Water research

Result: After the first detections of variants containing the N501Y mutation, the increase followed a trend that is comparable with the increase of N501Y-containing variants in patients in Baden-Wuerttemberg.

Result: In December 2020, the state of Baden-Wurttemberg reported the detection of the alpha variant (B.1.1.7) for the first time, corresponding to the first detection of N501Y in wastewater influent from Karlsruhe.

Result: Overall, RT-ddPCR and NGS are complementary methods that provide important information on the distribution of N501Y and other relevant mutations at the community level through the analysis of RNA extracts from wastewater samples.

Computational modelling of potentially emerging SARS-CoV-2 spike protein RBDs mutations with higher binding affinity towards ACE2: A structural modelling study.

PMID: 34979405 2022 Computers in biology and medicine

Introduction: These variants, such as B.1.17 (Alpha variant) harbouring a mutation N501Y reported in the UK, D614G

Result: Among the reported mutations, N501Y, E484K, K417 N, E484Q and L452R elevate the pathogenicity scale.

Result: In the recently reported mutations, including B.1.1.7 (N501Y), B.1.351, P.1, B.1.617 and B.1.618, increased in the stability increased was strongly correlated with a stable evolution of the new variants and tighter binding.

Engineered small extracellular vesicles displaying ACE2 variants on the surface protect against SARS-CoV-2 infection.

PMID: 34982509 2022 Journal of extracellular vesicles

Abstract: Furthermore, engineered sEVs inhibit the entry of wild-type (WT), the globally dominant D614G variant, Beta (K417N-E484K-N501Y) variant, and Delta (L452R-T478K-D614G) variant SARS-CoV-2 pseudovirus, and protect against authentic SARS-CoV-2 and Delta varia

Introduction: Further, pseudoviruses of the B1.351 lineage, also known as Beta variant, containing S protein receptor-binding domain (RBD) mutations, including N501Y, E484K, and K417N, exhibited increased resistance to neutralization by therapeutic monoclonal antibodies and convalescent plasma (Wibmer et al.,).

Developing an Amplification Refractory Mutation System-Quantitative Reverse Transcription-PCR Assay for Rapid and Sensitive Screening of SARS-CoV-2 Variants of Concern.

PMID: 34985323 2022 Microbiology spectrum

Introduction: Another study revealed that, due to mutations K417N, E484K, and N501Y in the spike protein of B.1.351, convalescent and some vaccine sera might only offer limited protection against B.1.351.

Introduction: Variants belonging to lineages B.1.1.7 and B.1.351 were found to have an N501Y amino acid substitution.

Result: It is worth noting that the hot spot amino acid substitutions currently of interest, such as N501Y, E484K, and P681R, were not unique or specific mutations able to differentiate the VOCs.

A bivalent nanoparticle vaccine exhibits potent cross-protection against the variants of SARS-CoV-2.

PMID: 34990583 2022 Cell reports

Introduction: Given that the B.1.351 and P.1 strains harbor K417N/T, E484K, and N501Y mutations in the RBD domain, B.1.526 harbors E484K, and B.1.617.1 harbors L452R and E484Q (Figure S4C), we wanted to develop a D614G- and E484K/Q-specific bivalent vaccine.

Different decay of antibody response and VOC sensitivity in naive and previously infected subjects at 15 weeks following vaccination with BNT162b2.

PMID: 34998405 2022 Journal of translational medicine

Discussion: reported that the B.1.1.7 lineage (and B.1.525 lineage) bearing the N501Y single mutation in the RBD was robustly neutralized by vaccine-elicited antibodies from naive subjects, whereas neutralization of the B.351 and P.1 lineages was lower compared to that of the B.1 lineage, although robust.

Browser Board

Co-occurred Entities

Filtrator