Abstract: Variant A.27 is characterized by a mutational pattern in the spike gene that includes the L18F, L452R and N501Y spike amino acid substitutions found in various variants of concern but lacks the globally dominant D614G.
Result: L18F is present in the VOCs B.1.351 and P.1, the L452R substitution is found in high frequencies in B.1.617.2 and related AY lineages, and N501Y is known from B.1.1.7, B.1.351 and P.1.
Result: Furthermore, N501Y was suggested to enhance the binding affinity to ACE2.
Result: Lineage A.27 has two mutations in its Table: N501Y
Evolution of the SARS-CoV-2 spike protein in the human host.
Abstract: Spikes of both variants have the same mutation, N501Y, in the receptor-binding domains.
Method: The following variant spikes were made (SA, Alpha and mink) (all mutations listed with reference to the NCBI sequence YP_009724390.1): 2P Alpha (Delta69-70, Delta144, N501Y, A570D, D614G, P681H, T716A, S982A, D1118H, and K986P, V987P), FUR2P Alpha (Delta69-70, Delta144,
Figure: d The N501Y substitution present in both the Beta and Alpha variants allows formation of a new hydrogen bond or a salt bridge.
Using genomic epidemiology of SARS-CoV-2 to support contact tracing and public health surveillance in rural Humboldt County, California.
5Result: Notably, N501Y is present in three ""variant of concern"" lineages: B.1.1.7, B.1.351, and P.1."
Result: Detection of de novo N501Y emergence within a skilled nursing facility outbreak.
Result: Genomic surveillance of the outbreak in Facility A also revealed the emergence and eradication of a lineage that had a de novo asparagine to tyrosine substitution in site 501 of the Spike protein (N501Y).
Result: Of 89 samples sequenced during this outbreak, 16 samples shared this N501Y substitution.
Result: The first sample containing this N501Y substitution was collected on November 29, 2020.
Result: Within this clade, 14 of the 16 sequences differed from the primary outbreak strain in Facility A by only the
Omicron variant (B.1.1.529) of SARS-CoV-2: understanding mutations in the genome, S-glycoprotein, and antibody-binding regions.
Figure: In the figure, red arrows in a, c, e, and g show the K417N, T478K, E484A, and N501Y mutations found in the Omicron variant.
Figure: Red box in b, d, f, and h represents the county of origin of the K417N, T478K, E484A
Discussion: The significant mutations and features are N501Y (augments the binding between of S-protein and ACE2); D614G (increase infectivity); H655Y (accelerate transmission); N679K (increase viral transmission); and P681H (enhance binding affinity of S-protein) (Table 2).
Identifying SARS-CoV-2 Variants of Concern through saliva-based RT-qPCR by targeting recurrent mutation sites.
Result: including N501Y in both Alpha and Beta, E484K in Eta and Beta, K417N in Beta, and L452R and T478K only in Delta.
Discussion: Amino acid changes in spike proteins of variants contribute to immune evasion, and it has been suggested that N501Y is associated with increased infectivity, whereas L452R, T478K, and E484K with K417N reduce the interaction of neutralizing antibodies with RBD.
Insights into the structure and dynamics of SARS-CoV-2 spike glycoprotein double mutant L452R-E484Q.
Introduction: All three variants have the N501Y mutation, and its presence has been associated with increased transmissibility (Leung et al.; Zhao et al.).
Introduction: Another variant, B.1.351 (Mwenda et al.) and P.1 variant (Francisco et al.) carries 9 and 11 spike protein mutations, respectively, including 3 mutations in the receptor-binding domain (RBD), K417N/T, E484K, and N501Y.
Characterization of SARS-CoV-2 Variants B.1.617.1 (Kappa), B.1.617.2 (Delta), and B.1.618 by Cell Entry and Immune Evasion.
Introduction: In addition, spike with the N501Y mutation has gained the ability to utilize mouse ACE2 as the receptor to infect the mouse, expanding its host range.
Introduction: Subsequently, the N501Y mutation found in the B.1.1.7, B.1.351, and B.1.1.28.1 spike proteins has increased the binding affinity between the receptor-binding domain (RBD) and ACE2, increasing viral fitness and infectivity.
Result: In addition, the N501Y mutation in the spike has been demonstrated to have gained the ability to utilize mouse ACE2 for cell entry, and our data suggested that the N501Y mutant specifically gained the ability to utilize mouse ACE2 for cell entry, with an efficiency approximately 4-fold higher than that o
Tetra-primer ARMS-PCR combined with dual-color fluorescent lateral flow assay for the discrimination of SARS-CoV-2 and its mutations with a handheld wireless reader.
Abstract: Herein, we report a low-cost, facile, and highly sensitive diagnostic platform that can simultaneously distinguish wild-type (WT) SARS-CoV-2 and its two mutations, namely, D614G and N501Y, within 2 h.
Abstract: The WT and M viruses were indicated and were strictly discriminated by the presence of a green or red band on test line 1 for the D614G site and test line 2 for the N501Y site.
Evolutionary history of the SARS-CoV-2 Gamma variant of concern (P.1): a perfect storm.
PMID: 35266951
2022
Genetics and molecular biology
Introduction: Currently, the WHO has identified the Gamma lineage (B.1.1.28.1, P.1 or Gamma; Nextstrain clade 20J/V3) with the following key S mutations: L18F, T20N, P26S, D138Y, R190S, K417T, E484K, N501Y, D614G, H655Y, T1027I, and V1176F.
Introduction: Nine key mutations involving the S glycoprotein have been
Table: N501Y
Discussion: Other derived lineages also harbor the N501Y mutation, signaling its recurrence.
SARS_CoV_2 mutation literature information.
PMID: 
2022
Nature communications
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