Abstract: Variants of JS7, such as E484K or N501Y, can be quickly synthesized in pure form in large quantities by automated methods.
Figure: Three circulating human variants in the JS7 sequence, L452R (recent California), E484K and N501Y are indicated by red arrows, yellow side chains in the structure above.
Discussion: Although the N501Y mutant did not alter neutralization by polyclonal antibodies in some test sera, other changes in this area at the interface of the RBD and its ACE2 receptor.
Detection of R.1 lineage severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) with spike protein W152L/E484K/G769V mutations in Japan.
Introduction: The hallmark mutation shared by the B.1.1.7, B.1.351, and P.1 lineages is N501Y, located in the receptor-binding domain (RBD) of the spike protein.
Result: We previously identified P.1 lineage SARS-CoV-2, which harbors the K417T/E484K/N501Y mutations, in one patient.
Nanobodies from camelid mice and llamas neutralize SARS-CoV-2 variants.
Introduction: A second variant of concern is B.1.351, which combines N501Y with two additional RBD substitutions (K417N and E484K).
Introduction: In contrast to their efficacy against the wild
Method: The mutants E484K and KEN (K417N, E484K and N501Y) were constructed in the context of a pSARS-CoV-2-SDelta19 variant with a substitution in the furin cleavage site (R683G).
Figure: Pseudotyped viruses containing E484K or K417N, E484K and N501Y (KEN) also contain the R683G substitution.
Characterization of SARS-CoV-2 different variants and related morbidity and mortality: a systematic review.
PMID: 34103090
2021
European journal of medical research
Table: N501Y
V367F Mutation in SARS-CoV-2 Spike RBD Emerging during the Early Transmission Phase Enhances Viral Infectivity through Increased Human ACE2 Receptor Binding Affinity.
Discussion: For example, through deep mutation scanning, an assumed N501F mutation was confirmed to have enhanced binding efficiency while not emergent, but later, the N501Y mutation in the same position with a similar enhancing effect was frequently detected in emerging lineage B1.1.7 variants.
FnCas9-based CRISPR diagnostic for rapid and accurate detection of major SARS-CoV-2 variants on a paper strip.
Method: A region from the SARS-CoV-2 S gene containing N501Y/T716I/E484K mutations was reverse transcribed and amplified using a single end 5' biotin-labeled primer.
Method: For the N501Y detection using RAY, SARS-CoV-2 and control RNA samples were received from the diagnostic laboratory at CSIR-Institute of Genomics and Integrative Biology.
Result: Among the four amplicon sizes that we investigated, an amplicon of 580 bp gave a clear discernible signal distinguishing the N501Y mutation over the WT sample (Figure 3A, Materials and methods).
Result: Among these SNVs, N501Y is present across 3/5 of the VOCs (B.1.1.7, P.1, and B.1.351).
Result: Importantly, amplicons containing the E484K mu
SARS-CoV-2 Brazil variants in Latin America: More serious research urgently needed on public health and vaccine protection.
PMID: 34109031
2021
Annals of medicine and surgery (2012)
Abstract: The essential modifications located in the spike glycoprotein RBD include E484K, K417T and N501Y.
Introduction: One of the significant mutations in N501Y was noted in RBD.
Introduction: The essential mutations in the spike include the glycoprotein RBD, E484K, K417T, and N501Y.
SARS-CoV2 spike protein gene variants with N501T and G142D mutation-dominated infections in mink in the United States.
PMID: 34109885
2021
Journal of veterinary diagnostic investigation
Introduction: Comparatively, the incidence of N501Y is much lower in the United States and Canada than globally.
Introduction: However, it cannot be ruled out that the different time of introduction of N501Y and N501T (April 2020 vs.
Introduction: In the GISAID (Global Initiative on Sharing All Influenza Data, https://www.gisaid.org) database of SARS-CoV2 genomes as of March 17, 2021, 758,684 of 792,388 (95.7%) of the SARS-CoV2 genome had the D614G mutation in the S protein gene; and 178,186 of 792,388 (22%) had the N501Y mutation, which occurred in the more virulent U.K.
Introduction: Our analysis suggested that the N501T mutants might have weaker transmissibility compared to the N501Y mutants,
Therapeutic effect of CT-P59 against SARS-CoV-2 South African variant.
PMID: 34119826
2021
Biochemical and biophysical research communications
Introduction: For instance, the B.1.1.7 (501Y.V1) variant first described in the UK, and containing a N501Y mutation in the spike protein has spread to more than 100 countries worldwide.
Introduction: In contrast, LY-CoV555 showed preserved activity against the UK variant but activity against the SA variant was completely abolished since LY-CoV555 cannot bind to triple mutant protein (K417N/E484K/N501Y) of RBD in SARS-CoV-2 SA variant.
Introduction: The second variant of concern (VOC), B.1.351 (501Y.V2) first discovered in South Africa variant, has 9 mutations (L18F, D80A, D215G, Delta242-244, K417N, E484K<
Potent RBD-specific neutralizing rabbit monoclonal antibodies recognize emerging SARS-CoV-2 variants elicited by DNA prime-protein boost vaccination.
SARS_CoV_2 mutation literature information.
PMID: 
2021
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