Introduction: (a) N501Y mutation, at the 501st amino-acid position of the spike protein, the amino acid asparagine is replaced by the amino acid tyrosine, is located within the RBD and can increase ACE2 receptor affinity.
Introduction: Both the B.1.351 variant and the B.1.1.28 variant have N501Y, E484K, and K417N mutations in the spike protein.
Introduction: Both the South African variant and the Brazilian variant have the N501Y mutation.
Introduction: In some studies, N501Y mutation has been found to be associated with a higher viral load and faster spread, which may be concerning to higher transmissibility.
Introduction: Like the South African varian
Corilagin prevents SARS-CoV-2 infection by targeting RBD-ACE2 binding.
Introduction: For instance, more contagious variants of SARS-CoV-2 with point mutations such as D614G, N501Y and E484K were identified with the abilities to induce immune escape.
Multiplex SARS-CoV-2 Genotyping Reverse Transcriptase PCR for Population-Level Variant Screening and Epidemiologic Surveillance.
PMID: 34037430
2021
Journal of clinical microbiology
Abstract: We designed and analytically validated a one-step multiplex allele-specific reverse transcriptase PCR (RT-qPCR) to detect three nonsynonymous spike protein mutations (L452R, E484K, N501Y).
Abstract: We detected 1,567 L452R mutations (38.7%), 34 N501Y mutations (0.84%), 22 E484K mutations (0.54%), and 3 (0.07%) E484K plus N501Y mutations.
Molecular dynamics analysis of N-acetyl-D-glucosamine against specific SARS-CoV-2's pathogenicity factors.
Discussion: The findings of Cheng and colleagues provided a higher affinity to the human ACE2 receptor, among the mutations, are the N501Y, K417N, and E484K mutations.
Clinical outcomes in COVID-19 patients infected with different SARS-CoV-2 variants in Marseille, France.
PMID: 34044152
2021
Clinical microbiology and infection
Abstract: RESULTS: A total of 254 patients were infected with clade 20A (20AS), 85 with Marseille-1 (M1V), 190 with Marseille-4 (M4V) and 211 with N501Y (N501YV) variants.
Abstract: RESULTS: A total of 254 patients were infected with clade 20A (20AS), 85 with Marseille-1 (M1V), 190 with Marseille-4 (M4V) and 211 with N501Y (_) variants.
Introduction: Finally, the third episode due to N501Y variants (N501YV) previously identified in the UK, South Africa and Brazil started in early 2021.
Method: Those selected were sequences of 20AS obtained from respiratory samples collected before June 2020 (the predominant variant of the first epidemic in Marseille), sequences of the M1V or M4V (predominant variants during the second phase of the e
SARS-CoV-2 N gene dropout and N gene Ct value shift as indicator for the presence of B.1.1.7 lineage in a commercial multiplex PCR assay.
PMID: 34044153
2021
Clinical microbiology and infection
Introduction: For example, amino acid exchange N501Y in the SARS-CoV-2 spike protein (S) is present in all three mentioned lineages, whereas the additional deletion H69/V70 in the same protein i
Result: These analyses revealed that all of the samples which had previously yielded N gene-specific Ct shifts or dropouts (DeltaCt N/RdRp or N/S score > 6) belonged to the B.1.1.7 lineage, whereas the remaining 58 SARS-CoV-2 positive samples with DeltaCt N/RdRp or DeltaCt N/S < 6 were all classified as non-B.1.1.7 variants (B.1.1.317 (n=2), B.1.351 (n=1), N501Y+, E484K+ (n=1), N501Y-, E484K+ (n=1) and N501Y-, Del.
E484K as an innovative phylogenetic event for viral evolution: Genomic analysis of the E484K spike mutation in SARS-CoV-2 lineages from Brazil.
PMID: 34044192
2021
Infection, genetics and evolution
Introduction: N501Y is one of the key contact residues interacting with hACE2 and P681H is one of four residues comprising the insertion that creates a furin-like cleavage site between S1 and S2, which is not found in closely-related coronaviruses.
Introduction: Moreover, B.1.1.7, B.1.351, and P.1 also harbor N501Y mutation, which is associated with enhanced receptor affinity and increased infectivity and virulence in a mouse model.
Introduction: The presence of E484K and N501Y substitutions in the same SARS-CoV-2 genome may be particularly relevant for viral evolution.
Introduction: The second lineage probably emerged in South Africa in August 2020 and harbors three mutations in RBD: K417N, E484K
Recognition through GRP78 is enhanced in the UK, South African, and Brazilian variants of SARS-CoV-2; An in silico perspective.
PMID: 34049205
2021
Biochemical and biophysical research communications
Result: In the mutant RBD isoform, the N501Y may be responsible for better binding of the RBD to the host cell receptors, as we reported previously in the UK variant of SARS-CoV-2 (VOC-202012/01).
Result: On the other hand, the N501Y mutation is responsible for forming one H-bond and one hydrophobic contact in the mutated variants of SARS-CoV-2.
Result: Table 2 shows the docking scores and the interactions established upon docking of the ACE2 into SARS-CoV-2 spike RBD in WT and the 501.V2 variant (K417 N, E484K, and N501Y) without GRP78.
Result: The three mutations (
A core-shell structured COVID-19 mRNA vaccine with favorable biodistribution pattern and promising immunity.
PMID: 34059617
2021
Signal transduction and targeted therapy
Abstract: Extensive evaluations in mice and nonhuman primates revealed strong immunogenicity of SW0123, represented by induction of Th1-polarized T cell responses and high levels of antibodies that were capable of neutralizing not only the wild-type SARS-CoV-2, but also a panel of variants including D614G and N501Y variants.
Introduction: Moreover, antibodies elicited by SW0123 demonstrated high neutralizing potency against a panel of SARS-CoV-2 variants including the D614G and N501Y mutant that has emerged as the dominant variant with increased infectivity.
Discussion: The variants such as Y453F, N439K, N501Y, and E484K have shown either enhanced cross-species transmissibility or ability to escape neutr
Convalescent-Phase Sera and Vaccine-Elicited Antibodies Largely Maintain Neutralizing Titer against Global SARS-CoV-2 Variant Spikes.
Discussion: In our experiments, D614G caused a significant increase in infectivity, but the variants, all of which contain the mutation, showed either no increase or a small increase (1.1- to 1.6-fold) in infectivity (Delta69-70, S982A, and Delta69-70/N501Y/P681H).
Discussion: Viruses pseudotyped with B.1.1.7 or B.1.351 were not more infectious than the parental D614G, although in an ACE2 binding assay, the N501Y mutation present in the B.1.1.7, B.1.351, and COH.20G/677H spike
Discussion: found that BNT162b2 vaccine-elicited antibodies neutralized virus with E484K/N501Y mutations with a titer that was 0.8-fold lower compared to D614G.
SARS_CoV_2 mutation literature information.
PMID: 
2021
Frontiers in medicine
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