Discussion: The set of signature variants includes 8 changes from S protein: deletion 69-70, deletion 144-145, N501Y (A23063T), A570D (C23271A), D614G, P681H (C23604A), T716I (C23709T), S982A (T24506G), D1118H (G24914C).
Simultaneous evaluation of antibodies that inhibit SARS-CoV-2 variants via multiplex assay.
Discussion: A proportion of RBD-specific mAbs isolated from vaccinated individuals have also demonstrated significant neutralization loss (>50-fold) against N501Y mutants.
Discussion: However, there were differences in particular with S477N, which had the second highest overall affinity (KD) for ACE2 (after N501Y) as determined via BLI but had weaker EC50 compared with Q493L and S494P, respectively.
Discussion: Indeed, the frequency and presence of the N501Y mutation in recently rapidly emerging SARS-CoV-2 lineages, B.1.1.7, B.1.351, B.1.1.70, and P.1, appear to further strengthen this hypothesis, as does the frequency of S477N and S494P, which we find bound ACE2 with
Evolutionary insights into the furin cleavage sites of SARS-CoV-2 variants from humans and animals.
Introduction: The D614G mutation is thought to increase virus transmissibility, while the N501Y mutation has also been implicated in the rapid spread of SARS-CoV-2.
Discussion: Moreover, this mutation is also a characteristic of the newly emerged SARS-CoV-2 variants, which are defined by multiple mutations in the S glycoprotein (Delta69-70, Delta144, N501Y, A570D, D614G, P681H, T716I, S982A, and D1118H).
SARS-CoV-2 B.1.617 Indian variants: Are electrostatic potential changes responsible for a higher transmission rate?
Result: Starting with position 501 within a loop in the RDB interacting with the ACE2 receptor, we note that the mutation N501Y does not show any clear structural effect as the negative DeltaDeltaG values are very close to 0.0 kcal/mol in DynaMut.
Table: N501Y
Coding-Complete Genome Sequences of 11 SARS-CoV-2 B.1.1.7 and B.1.351 Variants from Metro Manila, Philippines.
Figure: (e) Asn501Tyr (N501Y) mutation shifts the binding pocket of tauro-alpha-muricholic acid (orange) and (f) tilivalline (yellow).
Figure: Asn501Tyr (N501Y) mutation abolishes the binding pocket of bile acids and NRPs in the WT-RBD.
Figure: The Asn501Tyr (N501Y) mutation is seen among many variants.
Figure: The N501Y mutation or other mutations interfere with the binding of NPs with the RBD and/or increase the interaction of RBD with the ACD2 receptor.
Figure: The new binding pocket has a significantly lower affinity for these NPs (Supporting Table S1) and in this pocket the interference of (g) tauro-alpha-mu
Detection of Representative Mutant Strains and a Case of Prolonged Infection by SARS-CoV-2 with Spike 69/70 Deletion in Japan.
Abstract: The mutants of the spike region of SARS-CoV-2, such as N501Y, E484K, P681H, and deletion H69/V70 (del 69/70), were studied in 25 COVID-19 patients admitted from December 2020 to April 2021; there were no patients with N501Y and P681H, but nine patients had E484K alone.
Introduction: E484K was found in South Africa and Brazil with the N501Y mutant, and they appear to be associated with decreased vaccine efficacy.
Introduction: In contrast, nine patients had the E484K mutation without N501Y an
Table: N501Y
Impact of temperature on the affinity of SARS-CoV-2 Spike for ACE2.
Abstract: Interestingly, the RBD N501Y mutation, present in emerging variants of concern (VOCs) that are fueling the pandemic worldwide, bypassed this requirement.
Abstract: This data suggests that the acquisition of N501Y reflects an adaptation to warmer climates, a hypothesis that remains to be tested.
Mutants of human ACE2 differentially promote SARS-CoV and SARS-CoV-2 spike mediated infection.
Abstract: The panel of ACE2 mutants also revealed altered ACE2 surface dependencies by the N501Y spike variant, including a near-complete utilization of the K353D ACE2 variant, despite decreased infection mediated by the parental SARS-CoV-2 spike.
SARS_CoV_2 mutation literature information.
PMID: 
2021
Nature communications
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