Discussion: Three of these mutations - K4017T, E484K, and N501Y - are present in the receptor-binding domain, which is critical for ligand-receptor interaction, and were similar to the three mutations detected in the South African B1.351 lineage.
Emergence in southern France of a new SARS-CoV-2 variant harbouring both N501Y and E484K substitutions in the spike protein.
Conclusion: Apparently, the N501Y mutation in sRBD provides significant edges for the virus to proliferate; therefore, continued studies on the interaction between sRBD and hACE2 are warranted for the development of treatments against COVID-19.
Conclusion: In a more recent study [14], it was further validated that N501Y together with N501F, N501W and N501V exhibited enhanced binding affinity between sRBD and hACE2 in vitro.
Conclusion: In summary, our in silico studies suggest that the N501Y mutation can enhance sRBD binding affinity with hACE2 and potentially cause the virus to evade antibody neutralization.
Conclusion: It only took the parental strain one passage to start exhibiting and accumulating the
Evidence of escape of SARS-CoV-2 variant B.1.351 from natural and vaccine-induced sera.
Abstract: The receptor-binding domain mutations provide tighter ACE2 binding and widespread escape from monoclonal antibody neutralization largely driven by E484K, although K417N and N501Y act together against some important antibody classes.
Introduction: All three recently identified variant SARS-CoV-2 strains have acquired mutations in the ACE2 interaction surface of the RBD: N501Y in B.1.1.7; K417N, E484K, and N501Y in B.1.351; and K417T, E484K, and N501Y in P.1.
Discussion: Interestingly, repeated use of plasma therapy in an immunocompromised individual led to the transient emergence of the <
SARS-CoV-2 501Y.V2 variants lack higher infectivity but do have immune escape.
Introduction: Compared against the S protein of the 614G virus shows that 501Y.V2-3's S protein c
Table: N501Y
Discussion: The lethal variant is characterized by the superposition of two RBD mutations, Q493H and K417N, in the N501Y mutant background.
Discussion: This enhanced adaptation to murine hosts is at least partially attributable to the occurrence of the N501Y mutation.
Discussion: We found that multiple pseudotyped viruses harboring N501Y and K417N mutations (including 501Y.V2-2 and 501Y.V2-3) were significantly more infective toward HEK293T cells expressing murine ACE2 compared to the reference 614G variant.
Multiple SARS-CoV-2 variants escape neutralization by vaccine-induced humoral immunity.
Result: A pseudovirus bearing only the three RBD mutations (K417N, E484K, and N501Y) largely, but not entirely, recapitulated the escape phenotype (Figures 5B and 5D).
Result: Despite this escape, antibodiesexhibited reduced, but detectable, binding to mutant RBD protein harboring B.1.351 mutations (K417N, E484K, and N501Y) by ELISA, which correlated with K417N+E484K+N501Y pseudovirus neutralization (R2 = 0.67, p < 0.0001) (Figure 5E).
Result: Similarly, neutralizing antibody responses were also significantly decreased for the Brazilian/Japanese P.1 strain (6.7-fold for BNT162b2, p < 0.0001; 4.5-
Reduced neutralization of SARS-CoV-2 B.1.1.7 variant by convalescent and vaccine sera.
Abstract: Variant B.1.1.7, now dominant in the UK, with increased transmission, harbors 9 amino acid changes in the spike, including N501Y in the ACE2 interacting surface.
Abstract: We map the impact of N501Y by structure/function analysis of a large panel of well-characterized monoclonal antibodies.
Emergence in southern France of a new SARS-CoV-2 variant harbouring both N501Y and E484K substitutions in the spike protein.
PMID: 33755190
2021
Journal of cellular physiology
Abstract: The behavior of the wild type and N501Y is comparable.
Introduction: The mutation N501Y co-occurs with other mutations in the N, orf8, orf1a, and S glycoprotein in 501, involving two deletions Delta69 and Delta70.
Introduction: The substitution mutations N501Y, E484K, and others) within RBD of the UK and South African SARS-CoV-2 strains are now spreading unchecked.
Result: At some intervals, the N501Y (ACE2-spike RBD) possesses similar behavior as the wild type over the course of the simulation.
Result: Figure 6a shows that th
Higher infectivity of the SARS-CoV-2 new variants is associated with K417N/T, E484K, and N501Y mutants: An insight from structural data.
PMID: 33755190
2021
Journal of cellular physiology
Introduction: Novel mutations in the spike protein of B.1.1.7 (deletion 69-70, 144 and substitution K417N
Figure: (c) show the individual residue flexibility, that is, K417N, K417T, E48K, and N501Y.
Figure: (c) wild type, (d) E484K, (e) N501Y, and (f) E484K-N501Y, (g) K417N-E484K-N501Y, and (h) shows the K417T-E484K-N501Y structure of the spike RBD.
Pan-India novel coronavirus SARS-CoV-2 genomics and global diversity analysis in spike protein.
Discussion: Recently, the emergent UK variant strain named as VUI-202012/01 with defined as a set of 17 mutations, more significant N501Y was also observed and reported in India.
Emergence in southern France of a new SARS-CoV-2 variant harbouring both N501Y and E484K substitutions in the spike protein.
Discussion: Although mutations in other parts of the genome could also contribute to this transmission phenotype, the N501Y substitution in the spike protein in particular appears to be a major determinant of efficient transmission.
Discussion: Combined with our data indicating that none except Delta69-70 and N501Y has a consistent phenotype, and some even show reduced fitness in our experimental results at certain timepoints, this suggests many if not all of the remaining mutations were not directly selected; these mutations probably occurred through drift mechanisms such as founder and hitchhiking effects, followed by maintenance via linkage to Delta69-70 and N501Y, or possibly via recombination, which occurs frequently during SARS-CoV-2 replication.
Discussion: Fortunately, tests to date with N501Y substitut
SARS_CoV_2 mutation literature information.
PMID: 
2021
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