literature

SARS_CoV_2 mutation literature information.

Interaction Analysis of the Spike Protein of Delta and Omicron Variants of SARS-CoV-2 with hACE2 and Eight Monoclonal Antibodies Using the Fragment Molecular Orbital Method.

PMID: 35312321 2022 Journal of chemical information and modeling

Result: In N501Y, the difference of dispersion energy between the crystal structure of the wild type and the Omicron variant was calculated to be 4.3 kcal/mol, which is the biggest among the other residues due to the T-shaped pi-pi stacking interaction between Tyr41 in hACE2 and Tyr501 in the S protein of the Omicron variant (Figure S2).

Result: In the crystal structure, S375F, N440K, S477N, T478K, E484A, Q493R, G496S, Q498R, and N501Y interacted more strongly with the omicron variant than with the wild type (Table 2).

Result: The Table: N501Y

Potential inhibitor for blocking binding between ACE2 and SARS-CoV-2 spike protein with mutations.

PMID: 35279013 2022 Biomedicine & pharmacotherapy

Abstract: In addition, GB-1 inhibited the binding between ACE2 and RBD with a single mutation (E484K or N501Y), except the K417N mutation.

Abstract: In the compositions of GB-1, glycyrrhizic acid can inhibit the binding between ACE2 and RBD with Wuhan type, except K417N-E484K-N501Y mutation.

Abstract: In this study, we discovered that GB-1, developed from Chiehyuan herbal formula which obtained from Tian Shang Sheng Mu of Chiayi Puzi Peitian Temple, can inhibit the binding between ACE2 and RBD with Wuhan type, K417N-E484K-N501Y and

Cross-Neutralizing Breadth and Longevity Against SARS-CoV-2 Variants After Infections.

PMID: 35281007 2022 Frontiers in immunology

Introduction: B.1.1.7, which was firstly detected in the United Kingdom, has an N501Y mutation in the receptor binding domain (RBD) of S protein.

Introduction: B.1.351, which was found in South Africa, has three mutations (K417N, E484K, and N501Y) in the RBD.

Introduction: Finally, B.1.1.529, which was detected in Botswana on November 11, 2021 and South Africa on November 14, 2021, has 15 mutations (G339D, S371L, S373P, S375F, K417N, N440K, G446S,

SARS-CoV-2 infection after vaccination in Italian health care workers: a case report.

PMID: 35283546 2022 National Academy science letters. National Academy of Sciences, India

Abstract: Their genotyping performed on RNA extracts highlighted the presence of del69/70, N501Y, A570D, and 1841A > G (D614G) sequence variants, all indicative of VOC 202012/01-lineage B.1.1.7, suggesting a common source of infection.

Result: The genotyping performed first by Real-time PCR and then confirmed by direct sequencing proved the presence of del69/70, N501Y, A570D, and 1841A > G (D614G) variants, indicative of VOC 202,012/01-lineage B.

Figure: Sections from the electropherograms showing the 69/70, N501Y, A570D, and D614G (a., b., c., d.) associated with SARS-CoV-2 Alfa Variant B.1.1.7.

Design of SARS-CoV-2 Variant-Specific PCR Assays Considering Regional and Temporal Characteristics.

PMID: 35285246 2022 Applied and environmental microbiology

Result: To further confirm whether the RT-qPCR results were correct, we conducted NGS analysis to examine eight mutation markers for the Alpha variant (S:Delta69/70, S:Delta144, S:N501Y, S:A570D, S:P681H, S:T716I, S:S982A, and S:D1118H) and six mutation markers for the Delta variant (S:T19R, S:Delta156/157, S:L452R,

[Detection SARS-CoV-2 (Coronaviridae: Coronavirinae: Betacoronavirus: Sarbecovirus) in children with acute intestinal infection in Nizhny Novgorod during 2020-2021].

PMID: 35293190 2022 Voprosy virusologii

Abstract: Analysis of the S-protein amino acid sequence of the strains studied showed the absence of the N501Y mutation in the 2020 samples, which is a marker for variants with a high epidemic potential, called variants of concern (VOC) according to the World Health Organization (WHO) definition (lines Alpha B.1.1.7, Beta B.1.351, Gamma P.1).

Analysis of SARS-CoV-2 variants B.1.617: host tropism, proteolytic activation, cell-cell fusion, and neutralization sensitivity.

PMID: 35293847 2022 Emerging microbes & infections

Result: Additionally, the mouse-adapted mutants N501Y, Q493K, and Q498H of the SARS-CoV-2 RBD also showed significantly increased binding affinity towards mouse ACE2.

Result: Notably, similar as K417N, N501Y, and E484K in the B.1.351 variant, RBD mutations in the B.1.617 variants also showed enhanced infectivity in mouse cells.

Targeted Sanger sequencing to recover key mutations in SARS-CoV-2 variant genome assemblies produced by next-generation sequencing.

PMID: 35294336 2022 Microbial genomics

Abstract: We applied the primer set and wet-laboratory protocol to sequence 222 samples that were missing positions with key mutations K417N, E484K, and N501Y due to poor coverage after NGS sequencing.

Abstract: We successfully sequenced 153 samples of 222 (69 %) using Sanger sequencing and confirmed the occurrence of key beta variant mutations (K417N, E484K, N501Y) in the S genes of 142 of 153 (93 %) samples.

Clinico-Genomic Analysis Reiterates Mild Symptoms Post-vaccination Breakthrough: Should We Focus on Low-Frequency Mutations?

PMID: 35308382 2022 Frontiers in microbiology

Table: N501Y

Circulation of SARS-CoV-2 Variants among Children from November 2020 to January 2022 in Trieste (Italy).

PMID: 35336187 2022 Microorganisms

Result: Finally, 1/32 sequence was the Omicron strain, with the following mutations in the RBD domain: T22882G (N440K), G22898A (G446S), G22992A (S477N), C22995A (T478K), A23013C (E484A), A23040G (Q493R), G23048A (G496S), A23055G (Q498R), A23063T (N501Y), T23075C (

Filtrator