Introduction: These variants are known to possess multiple mutations across the genome, including several in the S protein and its RBD, such as N501Y, E484K and K417N/T.
Mutation hotspots and spatiotemporal distribution of SARS-CoV-2 lineages in Brazil, February 2020-2021.
Introduction: L452R, E484K, N501Y, P681H/R) have been positively selected, since they may confer adaptive advantages leading to convergent evolution in different lineages spreading across multiple countries.
Introduction: Moreover, all three VOC lineages harbor N501Y mutation, already associated with enhanced receptor binding affinity, which could lead to increased infectiousness.
Introduction: Th
Introduction: The former (B.1.1.7) emerged in England in mid-September 2020 and it is characterized by 14 lineage-specific amino acid substitutions, especially N501Y (a key contact residue interacting with hACE2) and P681H (one of four amino acids comprising the insertion that creates a novel furin cleavage site between S1 and S2).
CAR-NK Cells Effectively Target SARS-CoV-2-Spike-Expressing Cell Lines In Vitro.
Abstract: S309-CAR-NK cells can specifically bind to pseudotyped SARS-CoV-2 virus and its D614G, N501Y, and E484K mutants.
Introduction: Since February 2020, the frequency of the SARS-CoV-2 D614G, N501Y, and E484K variants have increased significantly and has become the dominant variant over the course of the pandemic.
Method: 293T cells were transfected with 3.75 mug pSFG-SARS-CoV-2 S or pSFG-SARS-CoV-2 S D614G or E484K or N501Y, 2.5 mug RDF plasmid, 3.75 mug PegPam3 and Genejuice in 1 mL Opti-MEM medium for 48 hours at 37 C under 5% CO2.
Method: Generation of Stable A549-
Droplet digital RT-PCR to detect SARS-CoV-2 signature mutations of variants of concern in wastewater.
PMID: 34371414
2021
The Science of the total environment
Dis
Discussion: N501Y containing B 1.1.7 variants were first detected on December 82,020 in Amsterdam and two weeks later in Utrecht.
Discussion: Although more extensive research is needed to determine the limit of detection accurately, experiments on SARS-CoV-2 RNA from lineage B.1.351 (with mutation N501Y) and WT in different proportional mixtures demonstrated that proportions as low as 0,5% of lineage B.1.351 can be detected in a background of 700 RNA-copies of WT SARS-CoV-2.
Discussion: At the onset of this study in January 2021, the threat of emergence of B 1.1.7 (the Alpha VOC) in the Netherlands was most imminent and our data show that the emergence of this VOC was detectable via N501Y monitoring of wastewater.
Discussion: The proportion of N501Y variants in the weekly samples increased to approximately 80% on March 1, 2021.
Molecular Evolution and Epidemiological Characteristics of SARS COV-2 in (Northwestern) Poland.
Result: Co-existence of DeltaH69V70, DeltaY144, P681H, T716I, S982A, A570D, N501Y, and D1118H
Discussion: Our analysis shows that subsequently, three genetic lines of the SARS-CoV-2 molecular evolution have emerged, with the largest clade (Clade 1, lineages B.1.1.*) being the most diverse genetically, with a significant proportion (24.6% for this clade) of the VOC B.1.1.7 variant characterized by a signature combination of the DeltaY144, N501Y, A570D, S982A, D1118H, and T716I spike mutations.
Detection and characterization of the SARS-CoV-2 lineage B.1.526 in New York.
Precision Response to the Rise of the SARS-CoV-2 B.1.1.7 Variant of Concern by Combining Novel PCR Assays and Genome Sequencing for Rapid Variant Detection and Surveillance.
Introduction: For example, B.1.1.7 is characterized by a number of mutations in the S gene, including the H69/V70 and Y144 deletions, N501Y, and P681H, among a number of other changes.
Introduction: Sera from patients immunized with the Moderna or Pfizer-BioNTech mRNA vaccines exhibited a 1- to 3-fold decrease in neutralization activity against B.1.351, which carries both N501Y and E484K, which may indicate a degree of immune escape by this VOC.
Introduction: Th
Table: N501Y
Figure: The previous 7-day rolling average of SARS-CoV-2 positive specimens is represented in blue and the same day's previous 7-day rolling average of the number of specimens tested with both the DeltaH69/V70 and N501Y assays is represented in orange.
Cross-neutralization of RBD mutant strains of SARS-CoV-2 by convalescent patient derived antibodies.
Abstract: Most importantly, the antibodies from Bin2 showed stronger binding affinity or ability to mutant RBDs (N501Y, W463R, R408I, N354D, V367F, and N354D/D364Y) derive
Discussion: And some of them such as N501Y in the United Kingdom has been proven to increase the infectivity and became the dominant lineage.
Discussion: Meanwhile, although the sequence of HTS0433 and HTS0466 is almost identical, with only three amino acids different in frame region, the affinity to mutant N501Y is totally different, which demonstrated that these amino acids in frame region of VL is critical to bind with N501Y.
Acquisition of the L452R Mutation in the ACE2-Binding Interface of Spike Protein Triggers Recent Massive Expansion of SARS-CoV-2 Variants.
PMID: 34379531
2021
Journal of clinical microbiology
3Introduction: Because of the important functional and antigenic properties of the RBD, structural changes in this domain deserve special attention and have already been highlighted by such notorious RBD mutations as E484K (e.g., found in B.1.1.28, also known as the ""Brazil"" variant or VOI zeta) and N501Y (found in B.1.1.7, also known as the ""British"" variant or VOC alpha, and in B.1.351, also known as the ""South African"" variant or VOC beta)."
Effect of SARS-CoV-2 B.1.1.7 mutations on spike protein structure and function.
Abstract: The N501Y mutation introduces a pi-pi interaction that enhances RBD binding to ACE2 and abolishes binding of a potent neutralizing antibody (nAb).
SARS_CoV_2 mutation literature information.
PMID: 
2021
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