Targeting SARS-CoV-2 Receptor Binding Domain with Stapled Peptides: An In Silico Study.
PMID: 34114829
2021
The journal of physical chemistry. B
Method: Examples of such mutations are Asn439Lys (N439K) and Leu452Arg (L452R).
Recent progress on the mutations of SARS-CoV-2 spike protein and suggestions for prevention and controlling of the pandemic.
PMID: 34146731
2021
Infection, genetics and evolution
Abstract: Among these mutations, the most representative ones are substitution mutations such as D614G, N501Y, Y453F, N439K/R, P681H, K417N/T, and E484K, and deletion mutations of DeltaH69/V70 and Delta242-244, which confer the virus with enhanced infectivity, transmissibi
Figure: Among these mutations, the most representative ones are substitution mutations such as D614G, N501Y, Y453F, N439K/R, P681H, K417N/T, and E484K, and deletion mutations of DeltaH69/V70 and Delta242-244.
Rational optimization of a human neutralizing antibody of SARS-CoV-2.
PMID: 34147856
2021
Computers in biology and medicine
Table: N439K
Recurrent emergence of SARS-CoV-2 spike deletion H69/V70 and its role in the Alpha variant B.1.1.7.
Figure: (A and B) Maximum-likelihood phylogeny of global sequences carrying Spike mutant (A) N439K and (B) Y453F.
Figure: (A) Maximum-likelihood phylogeny of global SARS-CoV-2 whole-genome sequences, highlighting those with specific mutations in spike: DeltaH69/V70, N439K, Y453F, and N501Y.
Figure: (B and C) Cumulative occurrences of SARS-CoV-2 sequences with DeltaH69/V70 by month for (B) DeltaH69/V70 with or without N439K/Y453F and (C) DeltaH69/V70 with N501Y.
Figure: DeltaH69/V70 appears after spike N439K and Y453F
Analysis of SARS-CoV-2 variant mutations reveals neutralization escape mechanisms and the ability to use ACE2 receptors from additional species.
Result: Specifically, N501Y and Y453F combined with N439K increased affinity for human ACE2 by 5-fold (Figures 4D and S3 ).
Result: Using biolayer interferometry (BLI), we measured association and dissociation rates of the N501Y RBD mutant (B.1.1.7 carries that mutation as its sole RBD mutation), Y453F, as found in mink isolates, N439K, which is found in some European clades, a combination of Y453F and N439K, E484K (part of B.1.351 and P.1) as well as for the B.1.351 and the P.1 RBDs for a recombinant version of human ACE2 (Figures 4A, 4B, and 4D).
Table:
A Comprehensive Molecular Epidemiological Analysis of SARS-CoV-2 Infection in Cyprus from April 2020 to January 2021: Evidence of a Highly Polyphyletic and Evolving Epidemic.
Result: The sequences of lineage B.1.177 contained L18F, A222V and D614G mutations, and those of lineage B.1.258 contained DeltaH69/V70, N439K and D614G mutations/deletions.
Discussion: N439K lies within the RBD, has been reported to be associated with immune escape, and confers increased binding affinity upon the ACE2 receptor, resulting in infection with a similar clinical outcome and a marginally higher viral load.
Discussion: The independent evolution of certain mutations and deletions (DeltaH69/V70, N439K and K417N) in different lineages highlights the importance of molecular epidemiology studies, in which known and dangerous mutations can be identified and monito
The emerging SARS-CoV-2 variants of concern.
PMID: 34211709
2021
Therapeutic advances in infectious disease
Abstract: These mutations carry a lineage from N501Y, D614G, N439K, Y453F, and others, which are globally dominated by clades 20A, 20B, and 20C.
Method: N439K RBM mutation has independently emerged in multiple lineages, showing increased affinity in the spike protein for ACE2, resisting several monoclonal antibodies (a therapeutic approach), and escaping some polyclonal responses.
Method: N439K was found to be less infectious than variant D614G; however, N439K sti
Table: N439K
Discussion: N439K has a single amino acid change and has decreased sensitivity to neutralizing antibodies.
The Antigenicity of Epidemic SARS-CoV-2 Variants in the United Kingdom.
Abstract: The N501Y, N439K, and S477N mutations caused immune escape from nine of 18 mAbs.
Abstract: To determine whether the neutralization activity of monoclonal antibodies, convalescent sera and vaccine-elicited sera was affected by the top five
Discussion: N439K is the representative mutation of lineage B.1.258, in which it always appears together with the 69-70del mutation.
Discussion: As it is located in the RBD, the N439K mutation is also resistant to neutralization by some mAbs.
Discussion: In summary, this study found that the N501Y, N439K, and S477N mutations significantly decreased the neutralization activity of some monoclonal antibodies.
Antibody Cocktail Exhibits Broad Neutralization Activity Against SARS-CoV-2 and SARS-CoV-2 Variants.
Introduction: Variants B.1.141 and B.1.258 with mutation N439K increase spike affinity for ACE2 and confer resistance to several mAbs (Thomson et al.).
SARS_CoV_2 mutation literature information.
PMID: 
2021
The journal of physical chemistry. B
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