Impact of B.1.617 and RBD SARS-CoV-2 variants on vaccine efficacy: An in-silico approach.
PMID: 35370005
2022
Indian journal of medical microbiology
Table: N439K
Discussion: The mRNA-1273 vaccine's neutralizing activity towards number of variants like B.1.351, B.1.1.7 + E484K, B.1.1.7, P.1, B.1.427/B.1.429, D614G, 20A.EU2, 20E [EU1], N439K-D614G, and previously identified mutant in Denmark mink cluster 5 were identified and found to have the same neutrality level as Wuhan-Hu-1 (D1414).
The dynamics of circulating SARS-CoV-2 lineages in Bogor and surrounding areas reflect variant shifting during the first and second waves of COVID-19 in Indonesia.
Discussion: On the other hand, substitutions in the region encoding the spike protein in Indonesian lineages mainly consisted of three key mutations, namely S_D614G, S_N439K and S_P681R.
Discussion: The S_N439K mutation has been associated with a similar level of fitness and virulence to the wild-type virus, whilst potentially conferring a stronger binding affinity to human ACE2 receptor and allowing immune escape from antibody-mediated immunity .
Discussion: The presence of the S_N439K and S_ P681R substitutions in the Indonesian lineages are important to note, as these mutations have been shown to confer certain advantages for the variants.
Result: First, the 10 most observed or fast-growing RBD mutations are N501Y, L452R, T478K, E484K, K417T, S477N, N439K, K417N, F490S, and S494P, as shown in Table 1.
Table: N439K
Whole genome sequence analysis showing unique SARS-CoV-2 lineages of B.1.524 and AU.2 in Malaysia.
Discussion: Although N439K mutation in RBD was first found in already extinct lineage B.1.1.41, a new lineage B.1.258 independently acquired the same amino acid substitution.
Discussion: Higher infectivity of the SARS-CoV-2 variants is associated with increased in binding affinity between spike protein and ACE2 due to K417N, E484K, N439K and N501Y mutations in the RBD of the spike protein.
Discussion: However, there is no evidence of change in disease severity in a large cohort of patients infected with SARS-CoV-2 harbouring N439K mutation in the spike protein.
Discussion: It is unknown whe
Characterization of a Broadly Neutralizing Monoclonal Antibody against SARS-CoV-2 Variants.
Introduction: It has been reported that several mutations in the SARS-CoV-2 RBM, such as N439K, L452R, S477N, T478K, E484K, S494P, N501Y, and A502S, have increased the infectivity and stability of SARS-CoV-2.
A Detailed Overview of Immune Escape, Antibody Escape, Partial Vaccine Escape of SARS-CoV-2 and Their Emerging Variants With Escape Mutations.
Abstract: Simultaneously we have discussed the significant mutations related to emerging variants and immune escape, such as mutations in the RBD region (N439K, L452R, E484K, N501Y, K444R) and other parts (D614G, P681R) of the S-glycoprotein.
Introduction: 4.1.3.1 N439K.
Introduction: In this direction, we illustrated the mutations in the RBD region (N439K, L452R, E484K, N501Y, K444R) and other regions (
Circulation of SARS-CoV-2 Variants among Children from November 2020 to January 2022 in Trieste (Italy).
Abstract: Of the 32 isolates, 4 (12.5%) carried a nonsynonymous nucleotide mutation leading to the N439K (3/4), lineage B.1.258 ( H69/ V70), and S477N (1/4) substitution.
Result: A total of 6 out of 32 (18.8%) sequences showed a nucleotide point mutation leading to a synonymous amino acid substitution, while 4/32 (12.5%) sequences carried a total of 5 nucleotide mutations, 3 of which (C22879A) led to the N439K amino acid substitution and 1 of which (G22992A) led to the S477N amino acid substitution.
Result: Full genome sequences obtained by the NGS of a single SARS-CoV-2 isolated strain, N439K, and a single Delta-like strain, which were deposited at GISAID (N439K: EPI_ISL_7208675; Delta: EPI_ISL_7698
SARS-CoV-2 Mutations and Their Impact on Diagnostics, Therapeutics and Vaccines.
Introduction: Examples include the N501Y, S477N, N439K, D364Y and E484K substitutions identified in most VOCs, which correlate with higher transmissibility.
Impact of temperature on the affinity of SARS-CoV-2 Spike glycoprotein for host ACE2.
PMID: 34478710
2021
The Journal of biological chemistry
Result: S1), notably mutations L452R (8.8%), E484K (7.7%), T478K (5.9%), S477N (2.2%), and N439K (1.2%), which are also found in other various VOCs and were shown to either increase infectivity or promote the evasion of antibody responses.
Receptor binding, immune escape, and protein stability direct the natural selection of SARS-CoV-2 variants.
PMID: 34543625
2021
The Journal of biological chemistry
Introduction: These include 9 single site mutations K417N, N439K, Y453F, S477N, S477I, T478I, E484K, S494P and N501Y (Alpha variant), a double mutant (E484K/N501Y) and two triple mutants (K417N/E484K/N501Y (Beta variant) and
Result: Among 9 single site mutants, 2 mutants (N439K and S477I) did not express very well and appreciable amount of protein needed for binding studies could not be obtained for these mutants.
SARS_CoV_2 mutation literature information.
PMID: 
2022
Indian journal of medical microbiology
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