RT-qPCR Assays for Rapid Detection of the N501Y, 69-70del, K417N, and E484K SARS-CoV-2 Mutations: A Screening Strategy to Identify Variants With Clinical Impact.
PMID: 34123874
2021
Frontiers in cellular and infection microbiology
Discussion: This finding is important because in Mexico, previously, Hernandez-Huerta et al., only identified the D614G mutation in the spike protein and the L84S mutation in the ORF8 gene and Taboada et al., only reported that the lineages circulating in Mexico changed from late February to March from A2 to B1 but both two studies did not found new lineages establishment in this country.
Characterization of SARS-CoV-2 different variants and related morbidity and mortality: a systematic review.
PMID: 34103090
2021
European journal of medical research
Table: L84S
An Epidemiological Analysis of SARS-CoV-2 Genomic Sequences from Different Regions of India.
Result: Finally, the G-S variant, defined by the presence of the D614G and L84S mutations in the S and ORF8 proteins, respectively, was observed in four sequences of SARS-CoV-2 reported from Gujarat in June 2020.
Similarities and differences in the conformational stability and reversibility of ORF8, an accessory protein of SARS-CoV-2, and its L84S variant.
PMID: 34062392
2021
Biochemical and biophysical research communications
Result: 1D presents 1H NMR spectra for wild-type ORF8 and its L84S variant at 30C.
Result: 3, the peak intensity of the L84S variant at pH 6.2 was much greater than that of wild-type ORF8 at pH 6.1.
Result: After 7 days, the production of wild-type ORF8 and its L84S variant was confirmed in the culture medium.
Result: Conformation of the L84S variant.
Result: Following purification, wild-type ORF8 and its L84S variant in the culture medium were obtained as single proteins and both existed as dimeric forms.
Result: It was found that the denaturing temperature of L84S variant is slightly higher than that of
Mutation Signatures and In Silico Docking of Novel SARS-CoV-2 Variants of Concern.
Introduction: Based on GISAID classification, the clade L is considered as wild-type (WT) variant, which consists of initial isolates from China (including reference genome) or other countries mainly involved in the first stage of pandemic; the clade S is defined by L84S mutation in NS8; the clade V is defined by G251V mutation in NS3; the clade G is defined by D614G mutation in S-protein (dominant isolate since spring 2020).
Identification of E484K and other novel SARS-COV-2 variants from the Kingdom of Bahrain.
Result: ORF8 had one missense mutation at position T28144C that led to an amino acid change from Leucine to Serine (L84S).
Tracing genetic signatures of bat-to-human coronaviruses and early transmission of North American SARS-CoV-2.
PMID: 33966351
2021
Transboundary and emerging diseases
Result: Among these 21 SNPs, we also identified two previously reported SNPs, 8,782.C > T and 28,144.T > C (p-value = 4.03 x 10-28 and 9.73 x 10-33), resulting in a synonymous mutation and a missense mutation (Leu 84 Ser) (Tang et al., 2020) (Table 1).
Table: Leu84Ser
SARS-CoV-2 mutations: the biological trackway towards viral fitness.
Introduction: Among the accessory proteins, ORF3a and ORF8 are brought into limelight due to the rapid spread of cluster V (NSP3:F106F, RdRp:P323L, S:D614G and ORF3a:Q57H) and VI (NSP4:S76S and ORF8:L84S).
Introduction: Among the point mutations, L84S is the predominant one and associated with mild disease symptoms among the hospitalised individuals.
Introduction: Cluster I includes 3037C>T;
Impact of meteorological parameters and population density on variants of SARS-CoV-2 and outcome of COVID-19 pandemic in Japan.
SARS_CoV_2 mutation literature information.
PMID: 
2021
Frontiers in cellular and infection microbiology
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