Figure: (A and B) In vitro infection assay of VSV PsVs, including N501Y + D614G, N501Y + L452R + D614G, and N501Y + K417N +
Discussion: There are many potential explanations for the lower infectivity observed for the Los Angeles variant, including the theory that L452R drives enhanced transmissibility but not necessarily infectivity.
Discussion: This is further supported by our study regarding infectivity of PsVs incorporating mutations found in the India variant because the L452R mutation was also present and did not mediate enhancement of infection compared with the South Africa variant PsV.
Genomic reconstruction of the SARS-CoV-2 epidemic in England.
Introduction: In contrast to other VOCs, Delta/Kappa do not contain N501Y or E484K mutations, but their L452R mutation may reduce antibody recognition and P681R enhances furin cleavage, similar to the P681H mutation of Alpha.
Comprehensive mapping of binding hot spots of SARS-CoV-2 RBD-specific neutralizing antibodies for tracking immune escape variants.
Abstract: Conversely, double mutations E484Q and L452R present in B.1.617.1 variant show partial antibody evasion with no evidence for an additive effect.
Abstract: Of note, these escape mutations could not affect the ACE2 binding affinity of RBD, among which L452R even enhanced binding.
Abstract: The comprehensive escape mutation map not only confirms the widely circulating strains carrying important immune escape RBD mutations such as K417N,
Introduction: Recently, the B.1.617 variant carrying two RBD mutations (E484Q and L452R) emerged in India has become a variant of interest for its high transmission rate and ability to evade immune responses.
Spike protein evolution in the SARS-CoV-2 Delta variant of concern: a case series from Northern Lombardy.
Discussion: B.1.617.2 is resistant to bamlanivimab and moderately evades convalescent or BNT162b2-elicited sera, which is similar in magnitude to the loss of sensitivity conferred by L452R or E484Q alone.
The Emergence of the New P.4 Lineage of SARS-CoV-2 With Spike L452R Mutation in Brazil.
Abstract: While performing epidemiological and genomic surveillance of SARS-CoV-2 in samples from Porto Ferreira-Sao Paulo-Brazil, we identified sequences classified by pangolin as B.1.1.28 harboring Spike L452R mutation, in the RBD region.
Introduction: B.1.1.7 (Alpha), B.1.351 (Beta), Lineages P.1 (Gamma), and B.1.617.2 (Delta, a L452R mutant) are the current Variants of Concern (VOC).
Introduction: While doing epidemiological and genomic surveillance of SARS-CoV-2 in samples from the city of Porto Ferreira:SP:Brazil, using Sanger and NGS techniques, we detected a new lineage designated by Pango as P.4, harboring the L452R mutation, that is circulating in Sao Paulo state.
Method: The first is composed of complete genome sequences generated in this study along
Rapid Automated Screening for SARS-CoV-2 B.1.617 Lineage Variants (Delta/Kappa) through a Versatile Toolset of qPCR-Based SNP Detection.
Result: L452R was the only assay to show a severe delay in CT and would require further optimization.
Result: As part of this study, the following RT-PCR assays were evaluated for technical viability: E484K/Q, P681H/R, L452R, V1176F, del-Y144/145.
Result: The LoDs for individual targets were determined as follows: 182 IU/mL for L452R (95%CI: 1472-120 IU/mL), 144 IU/mL for P681R (95%CI: 197-91 IU/mL), and 79 IU/mL for E484Q (95%CI: 18.9-139 IU/mL) (Table 3).
Discussion: As a practical application of the experimental assays provided in this study, we compiled and provided clinical validation for a high-throughput multiplex-assay to specifically screen for B.1.617 lineage variants (del
Prior infection with SARS-CoV-2 boosts and broadens Ad26.COV2.S immunogenicity in a variant-dependent manner.
Method: SARS-CoV-2 pseudotyped lentiviruses were prepared by co-transfecting the HEK293T cell line with either the SARS-CoV-2 ancestral variant spike (D614G), the Beta spike (L18F, D80A, D215G, K417N, E484K, N501Y, D614G, A701V, 242-244 del) or the Delta spike (T19R, R158G L452R, T478K, D614G, P681R, D950N, 156-157 del) plasmids in co
Biological Significance of the Genomic Variation and Structural Dynamics of SARS-CoV-2 B.1.617.
Introduction: L452R is located in the RBD, and P681H or P681R is located in the furin cleavage site.
Introduction: It was observed that the variants carrying spike L452R change are likely to be more transmissible and infective and less susceptible to the neutralizing antibodies from convalescent patients and vaccine recipients.
Introduction: So
Result: We found that the B.1.617 viruses and H1 were distinguished by four non-synonymous mutations: U1355G (L452R), G1450C (E484Q), A1841G (D614G), and C2042G (P681R).
Functional Effects of Receptor-Binding Domain Mutations of SARS-CoV-2 B.1.351 and P.1 Variants.
Discussion: Instead of carrying the N501Y, all lineages within the B.1.617 variant include a leucine to arginine substitution at position 452 (L452R), that have been shown to increase affinity towards ACE-2, as well as infectivity and resistance to antibody-mediated neutralization in vitro.
Global Prevalence of Adaptive and Prolonged Infections' Mutations in the Receptor-Binding Domain of the SARS-CoV-2 Spike Protein.
Result: As mentioned earlier, L452R and T478K are signature mutations in the delta variant.
Result: Comparing the two timelines, the prevalence of L452R increased by roughly 56.07, 49.01 and 11.78% points in Asia, the UK and USA, respectively.
Result: During this period, N501Y, L452R and T478K mutations increased by 15.39, 23.06 and 26.75% points, respectively, while E484K, S477N, K417T and S494P mutations decreased by 1.35, 3.09, 0.37 and 0.88% points, respectively.
Result: Further, we observed a considerable prevalence of other adaptive mutations in the RBD, namely
SARS_CoV_2 mutation literature information.
PMID: 
2021
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