Mutations in the receptor-binding domain of human SARS CoV-2 spike protein increases its affinity to bind human ACE-2 receptor.
PMID: 35109768
2022
Journal of biomolecular structure & dynamics
Abstract: Here, we use the crystal structure of the RBD in complex with ACE-2 available in the public domain and analyse the 250 ns molecular dynamics (MD) simulations of wild-type and mutants; K417N, K417T, N440K, N501Y, L452R, T478K, E484K and S494P.
Pan-SARS neutralizing responses after third boost vaccination in non-human primate immunogenicity model.
Result: A number of these variants have RBD mutations such as L452R,
Discussion: Except for the Danish mink-adapted SARS-CoV-2 spillback, Y453F is found rarely in humans with very few isolates reported in GISAID and only a single report of the mutation arising in immunocompromised patients - this is despite Y453F having been shown in several studies to enhance human ACE2 binding, in a similar manner to the VOC-associated mutations N501Y or L452R.
Discussion: Here, we have demonstrated many VOCs, particularly Alpha/B.1.1.7 as well as those containing L452R (such as Delta/B.1.617.2), could have a fundamental fitness advantage in mink by increasing interaction with mustelid ACE2, compared with previous nonvariant strains.
SARS-CoV-2 infections in mRNA vaccinated individuals are biased for viruses encoding spike E484K and associated with reduced infectious virus loads that correlate with respiratory antiviral IgG levels.
Introduction: The Marseille-484K.V1, or R.1, lineage is another example of convergent evolution leading to the amino acid substitution E484K, as also observed for the substitutions L452R, N501Y, Q677H, and L18F, which have occurred independently in various SARS-CoV-2 lineages and geographical areas.
Multiplexed Lab-on-a-Chip Bioassays for Testing Antibodies against SARS-CoV-2 and Its Variants in Multiple Individuals.
Abstract: The concentrations of antibodies against RBD, D614G, N501Y, E484K, and L452R/E484Q-mutants after two doses of vaccines are 6.6 +- 3.6, 8.7 +- 4.6, 3.4 +- 2.8, 3.8 +- 2.8, and 2.8 +- 2.3 ng/mL, respectively.
Abstract: This suggests that neutralizing activities against N501Y, E484K, and L452R/E484Q-mutants were less effective than RBD and D614G-mutant.
Introduction: Each test can detect six targets (RBD, D614G, N501Y,
COVID-19 Delta variation; more contagious or more pernicious?
Introduction: Based on new findings, strains that contain L452R mutation can evade host immune response by escaping both cell and humoral immune systems, so it can be concluded that this virus is more dangerous and deadly.
Introduction: It was first classified in India in December 2020 and quickly established itself as the most common lineage within the country, leading to an ultimate increase in the number of cases and daily deaths and overburdening of health systems in April 2021 More detailed analysis disclosed that the prevailing lineage in distribution is a novel identified lineage B.1.617 holding in common signature mutations D111D, G142D, L452R, E484Q, D614G, and P681R, in the spike protein, containing with
Infection spread simulation technology in a mixed state of multi variant viruses.
Abstract: In this study, we successfully developed a novel screening assay using high-resolution melting analysis, in which two genotypes at G446/L452 and S477/T478 RBD were determined (G446S/L452 and S477N/T478K for Omicron; G446/L452R and S477/T478K for Delta).
Genomic characterization unravelling the causative role of SARS-CoV-2 Delta variant of lineage B.1.617.2 in 2nd wave of COVID-19 pandemic in Chhattisgarh, India.
Abstract: Delta variant spike mutation of T19R, G142D, E156G, L452R, and deletion (F157 and R158) helps in escaping antibody response, T478K and D614G enhance viral affinity with ACE2 receptor while P681R and D950N result in higher replication and transmissibility by cleaving S1/S2 at furin site.
Abstract: Similarly, the L452R mutation of B.1.617.2 (Delta) variant is present in A.2.5.
Introduction: Another variant, A.2.5, likely spread north from Central America to the Quebec province in Canada and accumulated deletions 141-143 at the NTD and the L452R mutations in RBD.
Introduction: Further analysis revealed the convergent acquisition of the D614G and L452R signature mutations of the B lineages and Delta variant, respectively, and a mutational jump of 23 mutations, a characteristic of most of the VOCs.
SARS_CoV_2 mutation literature information.
PMID: 
2022
Journal of biomolecular structure & dynamics
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