Discussion: With the evolution of VOCs and VOIs, the French recommendations evolved on 26 May 2021 to screen only E484K and two other mutations (E484Q, L452R), with an impact similar to E484K.
SARS-CoV-2 Spike Mutations, L452R, T478K, E484Q and P681R, in the Second Wave of COVID-19 in Maharashtra, India.
Discussion: L452R has been noted earlier in lineages B.1.427 and B.1.429 while the Discussion: A recent report revealed that the L452R mutation reduced or abolished neutralizing activity of 14 out of 35 RBD-specific mAbs including three clinical stage mAbs.
Discussion: A recent study revealed increased infectivity of the B.1.617 spike protein that could be attributed to L452R which itself caused a 3.5-fold increase in infectivity and, in combination with E484Q, caused a 3-fold increase.
Discussion: Another recent study has demonstrated that the mutation L452R can escape from human leukocyte antigen (HLA) 24-restricted cellular immunity and can also increase viral infectivity, potentially promoting viral replication.
dsmCRISPR: Dual synthetic mismatches CRISPR/Cas12a-based detection of SARS-CoV-2 D614G mutation.
Introduction: Additionally, the mutations of L452R and E484Q within spike receptor-binding domain (RBD) were shown to have reduced susceptibility to monoclonal antibodies, including bamlanivimab, as well as convalescent plasma, even though the exact mechanism is not well-established.
Discussion: The rapid spread of SARS-CoV-2 virus globally is reported to link with the occurrence of highly transmissive variants including D614G, E484Q, L452R, E484K etc.
Mutation hotspots and spatiotemporal distribution of SARS-CoV-2 lineages in Brazil, February 2020-2021.
Introduction: L452R, E484K, N501Y, P681H/R) have been positively selected, since they may confer adaptive advantages leading to convergent evolution in different lineages spreading across multiple countries.
Introduction: More recently, the B.1.617.2 lineage, first detected in India, has also been characterized as a VOC, primarily for carrying a constellation of mutations in the spike protein (especially L452R and P681R), its wide spread worldwide even outperforming other VOCs, and reduced antibody sensitivity in vaccinated individuals.
Droplet digital RT-PCR to detect SARS-CoV-2 signature mutations of variants of concern in wastewater.
PMID: 34371414
2021
The Science of the total environment
Abstract: It also indicates that RT-ddPCR could be used for sensitive and accurate monitoring of current (like K417N, K417T, E484K, L452R) or future mutations present in SARS-CoV-2 variants of concern.
Discussion: E484K, K417T, K417N or L452R) characteristic for various epidemiologically or immunologically relevant VoC's.
Molecular Evolution and Epidemiological Characteristics of SARS COV-2 in (Northwestern) Poland.
Introduction: Studies on the impact of the mutations on virus evolution are ongoing, and continuously identify novel variants and mutations, with the key recent ones being L452R, E484Q, and T478K from Indian isolate B.1.617-VOC Delta and Kappa.
Detection and characterization of the SARS-CoV-2 lineage B.1.526 in New York.
Result: A closely related lineage, B.1.526.1, is defined by spike mutations D80G, Delta144, F157S, L452R, T859N, and D950H.
Table: L452R
Precision Response to the Rise of the SARS-CoV-2 B.1.1.7 Variant of Concern by Combining Novel PCR Assays and Genome Sequencing for Rapid Variant Detection and Surveillance.
Discussion: Assays that target S gene mutations such as E484K, K417N, K417T, L452R, the Y144 deletion, and the 242 to 244 deletion, and the ORF1ab gene 3675 to 3677 deletion, are in development.
Discussion: describe the design and use of assays targeting N501Y, E484K, and L452R, which are all found in different VOCs; this approach allowed them to detect the rise of SARS-CoV-2 strains carrying L452R in the San Francisco Bay Area.
Acquisition of the L452R Mutation in the ACE2-Binding Interface of Spike Protein Triggers Recent Massive Expansion of SARS-CoV-2 Variants.
PMID: 34379531
2021
Journal of clinical microbiology
Discussion: Furthermore, it was shown that L452R confers viral escape from human leukocyte antigen (HLA)-restricted cellular immunity mediated by cytotoxic T lymphocytes.
Discussion: In particular, one study has shown that the L452R mutation reduced neutralizing activity of 14 of 34 RBD-specific monoclonal antibodies.
Discussion: Interestingly, it appears that the selection for L452R became especially strong relatively recently.
Discussion: It is impossible to say at this point to what extent the isolation of the CAL.20A variant is connected to possibly distinctive biological properties of the strain and, specifically, the L452R mutation.
Discussion: It was found that L452R reduces multifold the spike protein reactivity w
GB-2 blocking the interaction between ACE2 and wild type and mutation of spike protein of SARS-CoV-2.
Abstract: GB-2 inhibited the binding between ACE2 and the RBD with a single mutation (K417N or N501Y or L452R) except the E484K mutation.
Abstract: Our results suggest that GB-2 could be a potential candidate for the prophylaxis of some SARS-CoV-2 variants infection in the further clinical study because of its inhibition of binding between ACE2 and RBD with K417N-E484K-N501Y mutations or L452R mutation.
Abstract: The L452R mutation in the epsilon variant (the B.1.427/B.1.429 variants) also reduced neutralizing activity of monoclonal antibodies.
SARS_CoV_2 mutation literature information.
PMID: 
2021
Diagnostics (Basel, Switzerland)
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