Abstract: It is unclear whether antibody responses to SARS-CoV-2 infection or to the prototypic Wuhan-1 isolate-based vaccines will be impacted by the three B.1.427/B.1.429 S mutations: S13I, W152C and L452R.
Abstract: The RBD L452R mutation reduced or abolished neutralizing activity of 14 out of 35 RBD-specific monoclonal antibodies (mAbs), including three clinical-stage mAbs.
Complete map of SARS-CoV-2 RBD mutations that escape the monoclonal antibody LY-CoV555 and its cocktail with LY-CoV016.
Abstract: In addition, the L452R mutation in the B.1.429 lineage escapes LY-CoV555.
Result: In addition, the B.1.429 lineage (also known as 20C/CAL.20C) that has risen to high frequency in southern California contains L452R, which escapes LY-CoV555 (Figure 2B).
Result: Subsequent to the release of our original preprint version of this article, the US Food and Drug Administration's (FDA's) fact sheet for bamlanivimab EUA was updated, confirming our findings by noting that L452R reduces bamlanivimab neutralization >1,000-fold.
Result: The escape mutations present at the highest frequency among the sequenced isolates are E484K, L452R, and S494P for LY-CoV555 and K417N/T for LY-CoV016.
Result: This observation coincides
Detection and characterization of the SARS-CoV-2 lineage B.1.526 in New York.
Result: We note that the evolutionary history at spike position 701 varies depending on whether the tree is rooted using a molecular clock (Figure 1) versus its sister clade (characterized by an L452R mutation; Supplementary Figure 2), the latter of which posits a substitution A701V followed by a reversion V701A.
Post-vaccination SARS-CoV-2 infections and incidence of the B.1.427/B.1.429 variant among healthcare personnel at a northern California academic medical center.
Abstract: Chart extraction of demographic and clinical information was perfor
Result: Forty-two (36.5%) samples were positive for isolated L452R mutation, and were presumptive B.1.427/B.1.429 variant.
Result: In multivariate analysis, when controlling for community prevalence of L452R mutation the week prior to positive test (when exposure likely occurred), vaccination status at time of positive test was not significantly associated with presumptive B.1.427/B.1.429 (Table 3).
Result: In unadjusted analysis, PVSCs infected with L452R-containing viruses were more likely than those with no identified mutations to have tested positive after the second vaccine dose, or to be partially- or fully-vaccinated at time of positive test (Table 1); this variant was not associated with age, gender, job role, brand of vaccine received, immunocompromised status, or symptomatic infection.
Mutation Signatures and In Silico Docking of Novel SARS-CoV-2 Variants of Concern.
Discussion: As it can be seen, neither L452 nor R452 have a significant contribution to the total binding energy, however, L452R mutation is slightly in favor of complex formation in CAL-20C by lowering the binding energy (Supplementary Figure S1).
Discussion: The CAL-20C with L452R mutation in RBD is thought to be a more transmissible variant mainly due to epidemiological data (37% of all samples collected in California in January 2021 was CAL-20C).
SARS-CoV-2 mutations: the biological trackway towards viral fitness.
Introduction: 11 Virtually all variants of concern contain mutations in the SARS-CoV-2 Spike protein, such as variant B.1.351 (Spike mutations K417N, E484K, N501Y, D614G, and A701V) and variants B.1.427/B.1.429 (Spike mutations S13I, W152C, L452R, and D614G), and many of these reside on the receptor-binding domain (RBD), a region located between residues 350-550 of Spike and directly binding to the human ACE2.
Table: L452R
A Sanger-based approach for scaling up screening of SARS-CoV-2 variants of interest and concern.
PMID: 33975021
2021
Infection, genetics and evolution
Table: L452R
Spreading of a new SARS-CoV-2 N501Y spike variant in a new lineage.
PMID: 33991677
2021
Clinical microbiology and infection
Abstract: RESULTS: We identified that SARS-CoV-2 genomes from four patients diagnosed in our institute harboured a new set of amino acid substitutions including L18F, L452R, N501Y, A653V, H655Y, D796Y, G1219V +- Q677H.
Result: In addition, sequences that span codons 1-618 of the
Discussion: Its spike protein harbors several aa substitutions recently reported to emerge in various lineages and/or to be associated with immune escape (L18F, L452R, N501Y, H655Y, Q677H) (Supplementary Material Data).
Structural Modeling of the SARS-CoV-2 Spike/Human ACE2 Complex Interface can Identify High-Affinity Variants Associated with Increased Transmissibility.
Result: Consistent with this result, experimental analyses of L452R using deep mutagenesis and in vitro evolution do not suggest it produces an enhanced affinity relative to WT.
Result: Since both L452R and E484Q have significant antibody escape propensity (Figure 5(b)), our affinity analysis suggests the rapid spread of the L452R/E484Q variant likely stems from antibody resistance.
Result: The L452R mutation, also
Result: The double mutant L452R/E484Q has a strong favorable solvation energy but this is overcompensated by the entropic penalty of complex formation, resulting in a lower overall affinity relative to WT but similar to that for the L452R mutation.
SARS_CoV_2 mutation literature information.
PMID: 
2021
bioRxiv
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