Discussion: Among our panel of mutants, we isolated a total of 14 substitutions at sites of glycosylation, including eight N-linked glycans sites: T345N, K444N, S477N, L441R, L517R, L452R, S477R, and K444R; and six O-linked glycans: F486S, T345S, F490S, P479S, F486Y, and N450Y.
Discussion: For the wild-type S sequence and some escape mutants (e.g., L441R, K444N,
Multiple SARS-CoV-2 variants escape neutralization by vaccine-induced humoral immunity.
Introduction: Another variant that recently emerged in California, United States, designated as B.1.429, contains 4 missense mutations in spike, one of which is a single L452R <
Result: Separately, the B.1.1.298 variant found in Danish minks contained a Y453F mutation in RBD, and the California variant B.1.429 contained an L452R.
Result: When assessing variants containing one RBD mutation as part of their mutational landscape, the UK variant B.1.1.7 (N501Y), Danish mink variant B.1.1.298 (Y453F), and California variant B.1.429 (L452R) exhibited neutralization that was similar to that of wild-type and the parental D614G variant.
Complete map of SARS-CoV-2 RBD mutations that escape the monoclonal antibody LY-CoV555 and its cocktail with LY-CoV016.
Abstract: Additionally, the L452R mutation in the B.1.429 lineage escapes LY-CoV555.
Result: 20C/CAL.20C) that has risen to high frequency in southern California contains L452R, which escapes LY-CoV555.
Result: The escape mutations present at the highest frequency among sequenced isolates are E484K, L452R, and S494P for LY-CoV555, and K417N/T for LY-CoV016.
SARS-CoV-2 variants combining spike mutations and the absence of ORF8 may be more transmissible and require close monitoring.
PMID: 33676232
2021
Biochemical and biophysical research communications
Result: Eleven spike mutations were not found associated with ORF8 variants: L8V/W, N234Q, Q239K, L452R, A475V, G476S, V483A, F490L, V615I/F, A831V and D839Y/N/E.
Estimation of secondary household attack rates for emergent SARS-CoV-2 variants detected by genomic surveillance at a community-based testing site in San Francisco.
Abstract: Certain viral lineages bearing spike mutations, defined in part by L452R, S13I, and W152C, comprised 54.9% of the total sequences from January, compared to 15.7% in November.
Result: We observed SARS-CoV-2 genome sequences that belonged to PANGO lineages B.1.427 and B.1.429, both of which share a trio of recent mutations in the spike protein (S13I, W152C, and L452R) (Figure 2).
Discussion: In addition to the mutations associated with spike L452R in the West Coast variants, we observed, at lower frequencies, other mutations of interest, including those in spike at positions 677, and 681, bo
Neutralising antibody escape of SARS-CoV-2 spike protein: Risk assessment for antibody-based Covid-19 therapeutics and vaccines.
Abstract: The Spike protein has different hotspots of mutation and deletion, the most dangerous for immune escape being the ones within the receptor binding domain (RBD), such as K417N/T, N439K, L452R, Y453F, S477N, E484K, and N501Y.
Table: L452R
Multiple SARS-CoV-2 variants escape neutralization by vaccine-induced humoral immunity.
Introduction: Another variant that recently emerged in California, United States, designated as B.1.429, contains four missense mutations in spike, one of which is a single L452R RBD mutation.
Result: Separately, the B.1.1.298 variant found in Danish minks contained a Y453F mutation in RBD, and the California variant B.1.429 contained an L452R mutation.
Result: When assessing variants containing one RBD mutation as part of their mutational landscape, the UK variant B.1.1.7 (N501Y), Danish mink variant B.1.1.298 (Y453F), and California variant B.1.429 (L452R) exhibited neutralization that was generally similar to that of wild-t
Recombinant SARS-CoV-2 genomes are currently circulating at low levels.
Abstract: Recombinant genomes were also found to contain substitutions of concern for elevated transmissibility and lower vaccine efficacy, including D614G, N501Y, E484K, and L452R.
Acquisition of the L452R mutation in the ACE2-binding interface of Spike protein triggers recent massive expansion of SARS-Cov-2 variants.
Abstract: According to the phylogenetic analysis, however, emergence of CAL.20C was also specifically triggered by acquisition of the L452R mutation.
Abstract: Further analysis of GISAID-deposited genomes revealed that several independent L452R-carrying lineages have recently emerged across the globe, with over 90% of the isolates reported between December 2020 - February 2021.
Abstract: If true, this in turn might lead to significantly increased infectivity of the L452R variants, warranting their close surveillance and in-depth functional studies.
Abstract: In contrast to CAL.20C that carries two additional to L452R mutations in the Spike protein, L452R is the only mutation found in CAL.20A.
Abstract: In some samples, however, we found a distinct PMID: 33758899
2021
medRxiv
Abstract: Our analyses revealed 2-fold increased B.1.427/B.1.429 viral shedding in vivo and increased L452R pseudovirus infection of cell cultures and lung organoids, albeit decreased relative to pseudoviruses carrying the N501Y mutation found in the B.1.1.7, B.1.351, and P.1 variants.
Abstract: The variant carries 3 mutations in the spike protein, including an L452R substitution.
Introduction: Here we used viral
Method: SARS-CoV-2 spike mutants (D614G, D614G+W152C, D614G+L452R, and D614G+N501Y) were cloned using standard site-directed mutagenesis and PCR.
SARS_CoV_2 mutation literature information.
PMID: 
2021
Cell host & microbe
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