literature

Protective Immunity of the Primary SARS-CoV-2 Infection Reduces Disease Severity Post Re-Infection with Delta Variants in Syrian Hamsters.

PMID: 35337002 2022 Viruses

Introduction: AY.1 has amino acid substitutions at T19R, E156G, 157/158 del, W258L, K417N, L452R, T478K, D614G, D950N and P681R.

Introduction: The amino acid substitutions in the spike protein of the Delta variant, such as D614G, T478K, P681R and L452R, are known to affect transmissibility and neutralization.

Method: Delta AY.1 variant had D614G, E156G, F157del,

PMID: 35337800 2022 The Journal of biological chemistry

Method: SARS-CoV-2 S-protein RBD (wt), N501Y, L452R, and triple (K417T/E484K/N501Y) mutants were a gift from John Bates (University of Mississippi).

Result: 5D) and the Delta L452R mutant.

Result: Delta L452R.

E-Volve: understanding the impact of mutations in SARS-CoV-2 variants spike protein on antibodies and ACE2 affinity through patterns of chemical interactions at protein interfaces.

PMID: 35341044 2022 PeerJ

Int

Discussion: Alongside, the L452R alteration creates a highly repulsive interface involving the K106 residue in these models, further producing hydrogen bonds with the Y32 antibody amino acid.

Discussion: Furthermore, it also has gained highly frequent repulsive interactions in these residues caused by the L452R modification.

Identifying SARS-CoV-2 Variants of Concern through saliva-based RT-qPCR by targeting recurrent mutation sites.

PMID: 35262087 2022 medRxiv

Introduction: Additionally, L452R increases both structural stability and viral fusogenicity, and decreases cell-mediated immune response.

Introduction: Conveniently, the currently circulating Omicron variant (B.1.1.529) harbors both L452R and SDelta69-70, so we used these assays to quickly identify its emergence at Clemson University and the surrounding Upstate South Carolina in December 2021.

Introduction: We also evaluated the efficacy of TaqPath assays for K417T, E484K, E484Q, and L452R in saliva.

High throughput sequencing based direct detection of SARS-CoV-2 fragments in wastewater of Pune, West India.

PMID: 34688738 2022 The Science of the total environment

Conclusion: The observation of mutations L452R and E484Q associated with B.1.617.1 variant lineage in similar period of declaration as variant of concern provides a conclusive evidence of WBE as early warning system.

Result: Notably, nine mutations in the Spike region (S: L452R, S:C480R, S: E484Q, S: D614G, S: P681R, S: N801, S: D950N, S:

Table: L452R

Analysis of SARS-COV2 spike protein variants among Iraqi isolates.

PMID: 34754982 2022 Gene reports

Introduction: This variant is characterized by having several mutations in the spike proteins including L452R, T478K and P681R.

Result: Previously, the L452R variant was shown to become resistant to monoclonal antibodies (mAbs) X593 and P2B-2F6, however, mutating leucine (L) to glutamine (Q) or methionine (M) among the Iraqi strains could affect differently on the sensitivity of the strain towards the host and/or vaccine-induced immunity.

A low-cost TaqMan minor groove binder probe-based one-step RT-qPCR assay for rapid identification of N501Y variants of SARS-CoV-2.

PMID: 34656702 2022 Journal of virological methods

Introduction: Our laboratory is developing another TaqMan MGB probe-based one-step RT-qPCR assay to detect the presence of L452R mutation, which shall be used in conjunction with N501Y RT-qPCR.

Introduction: The major limitation of the assay is its inability to detect the recently expanding SARS-CoV-2 variant of concern, B.1.617.2 lineage, (a.k.a G/452R.V3), which does not harbour N501Y mutation but E484Q and L452R instead.

Gasless laparoendoscopic single-site surgery for management of unruptured tubal pregnancy in a woman with moderate COVID-19 pneumonia after administration of remdesivir and casirivimab-imdevimab: A case report.

PMID: 34786352 2022 Case reports in women's health

Abstract: Real-time reverse transcription-polymerase chain reaction assay detected SARS-CoV-2, which was subsequently identified to be an L452R variant.

Conclusion: Real-time reverse transcription polymerase chain reaction assay (RT-PCR) was positive for SARS-CoV-2, which was subsequently shown to be an L452R variant.

Longitudinal analysis of SARS-CoV-2 spike and RNA-dependent RNA polymerase protein sequences reveals the emergence and geographic distribution of diverse mutations.

PMID: 34801754 2022 Infection, genetics and evolution

Result: 5D) share the same set of mutations on the spike protein (S13I,

Figure: L18F, E484K, N501Y, D614G, H655Y, and V1176F are associated with the Gamma variant of concern, and S13I, W152C, L452R are associated with the Epsilon variant of concern.

Figure: The substitution L452R is observed in the Epsilon variant of concern.

Reduced sensitivity of the SARS-CoV-2 Lambda variant to monoclonal antibodies and neutralizing antibodies induced by infection and vaccination.

PMID: 34818119 2022 Emerging microbes & infections

Introduction: The L452R mutation found in the Delta, Kappa, and Epsilon variants has been reported to enhance viral infectivity and fusogenicity while promoting viral replication.

Discussion: In addition, our previous studies revealed that escape of the L452R and E484 K mutants from mAbs frequently occurred in conjunction.

Discussion: Our previous study found that the L452R and F490L mutations can cause escape from mAb X593, consistent with the results of the L452Q and F490S mutations in the present study.