Discussion: Although the results show an increase in infectivity close to 20-fold for the D614G/L452R protein, the increase in infectivity for D614G/W152C was fourfold when compared to the S protein containing only the D614G mutation.
Discussion: The VOI B.1.429, which also has a very important S:L452R mutation, was identified
Discussion: The infective capacities of mutations occurring in the B.1.429 variant were investigated using pseudoviruses carrying the D614G mutation together with the L452R or W152C mutations infected in 293 T cells expressing the ACE2 receptor as well as the cofactor TMPRSS2.
SARS-COV-2 Delta variant displays moderate resistance to neutralizing antibodies and spike protein properties of higher soluble ACE2 sensitivity, enhanced cleavage and fusogenic activity.
Abstract: Against AY.1 pseudoviruses, the L452R and K417N substitutions accounted for the loss of neutralization by four antibodies and one antibody, respectively, whereas one antibody lost potency that could not be fully accounted for by a single RBD substitution.
Abstract: Four of twenty-three therapeutic neutralizing antibodies showed either complete or partial loss of neutralization against B.1.617.2 pseudoviruses due to the L452R substitution, whereas six of twenty-three therapeutic neutralizing antibodies showed either complete or partial loss of neutralization against B.1.617.1 pseudoviruses due to either the E484Q or L452R substitution.
Abstract: The spike proteins of B.1.617.1, B.1.617.2, and AY.1 variants have several substitutio
SARS-CoV-2 Delta (B.1.617.2) Variant: A Unique T478K Mutation in Receptor Binding Motif (RBM) of Spike Gene.
Introduction: SARS-CoV-2 delta variant shares three common mutation sites, L452R, D614G, and P681R with SARS-CoV-2 kappa and B.1.617.3 variant (Table 1).
Introduction: The L452R mutation site presents in three SARS-CoV-2 epsilon and iota variants from US.
Method: Among them E484Q and L452R, that are present in both SARS-CoV-2 kappa, and B.1.617.3 variants of interest and alert.
Method: Astonishingly, critical protein structure studies were not able to identify the unique T478K mutation site of delta variant and the common L452R mutation site of delta, epsilon, iota, kappa, and B.1.617.3 variants.
Table: L452R
Introduction and rapid dissemination of SARS-CoV-2 Gamma Variant of Concern in Venezuela.
PMID: 34800714
2021
Infection, genetics and evolution
Introduction: Delta VOC harbors several mutations, such as L452R and T478K, being the former associated with an increased transmission potential and reduced susceptibility to protective immunity, both at humoral and cellular level.
Discussion: However, the smaller fragment used in this study is still useful for us, for an even more rapid detection (in one day) of the most frequent variants circulating in Venezuela, by restriction enzyme analysis to detect E484K and L452R mutations.
Implication of SARS-CoV-2 Immune Escape Spike Variants on Secondary and Vaccine Breakthrough Infections.
Abstract: This article highlights the pressures that facilitate the rise of new SARS-CoV-2 variants and the key mutations of the viral spike protein - L452R,
Introduction: A report of breakthrough infections in fully or partially vaccinated healthcare workers and a secondary infection with L452R-carrying variants have been described, highlighting the relevance of this mutation in mediating viral immune escape.
Introduction: Another mutation that has gained attention is L452R substitution which can resist neutralisation by monoclonal antibodies and vaccinee and convalescent sera.
Introduction: Other mutations, in addition to L452R of the RBD, have been attributed to its explosive spread.
Table: L452R
Mutation-Induced Long-Range Allosteric Interactions in the Spike Protein Determine the Infectivity of SARS-CoV-2 Emerging Variants.
Abstract: To address this question, here we have individually assessed the effects of SARS-CoV-2 variant-specific spike (S) protein receptor-binding domain (RBD) mutations E484K, K417N, L452Q, L452R, N501Y, and T478K that characterize and differentiate several emerging variants.
Introduction: Here, we have considered prominent spike receptor-binding domain (RBD) mutations that are commonly observed among the VOI/VOC variants (Table 1), namely, E484K, K417N, L452Q, L452R
A rigorous framework for detecting SARS-CoV-2 spike protein mutational ensemble from genomic and structural features.
PMID: 34806033
2021
Current research in structural biology
Result: A single amino acid substitution L452R is present in multiple lineages, including B.1.526, B.1.427, B.1.429, B.1.617.1, B.1.617.3, and lineages designated as Delta (B.1.617.2, AY.1, AY.2, and AY.3).
Result: Further, for the characteristic RBD mutations across VoCs (K417 T/N, L452R, S477N, T478K, E484K, and N501Y), we observed MTR scores between 0.68 and 0.91, suggesting these substitutions to be tolerant.
Result: The RBD of B.1.617.2 harbors two defining mutations (L452R and T478K), out of which L452R substitution enhanced the local hydrogen bond
Mechanistic insights into the effects of key mutations on SARS-CoV-2 RBD-ACE2 binding.
PMID: 34806722
2021
Physical chemistry chemical physics
Abstract: In this study, we provide atomic-level insights into the binding of the receptor binding domain (RBD) of the wild-type SARS-CoV-2 spike protein and its single (N501Y), double (E484Q, L452R) and triple (N501Y, E484Q, L452R) mutated variants to the human ACE2 receptor.
Abstract: We find higher binding affinities for the double (E484Q, L452R) and triple (N501Y, E484Q, L452R) mutated variants than for the wild type and the N501Y variant, which could contribut
Relative Consolidation of the Kappa Variant Pre-Dates the Massive Second Wave of COVID-19 in India.
8Discussion: By virtue of sharing three critical mutations in the spike protein with the delta variant (L452R, E484Q and P681R), the kappa variant can be considered to be the ""bridging variant"" that acted as the forerunner of the highly transmissible delta variant that was responsible for the enormity of the second wave in India."
Abstract: This relative consolidation of the kappa variant was significant, since it shared 3 of the 4 signature mutations (L452R, E484Q and P681R) observed in the spike protein of delta variant and thus was likely to be the precursor in its evolution.
Discussion: Furthermore, L452R mutation is reported to reduce neutralization activity of
A Strategy to Detect Emerging Non-Delta SARS-CoV-2 Variants with a Monoclonal Antibody Specific for the N501 Spike Residue.
Result: We also tested RBDs corresponding to delta, kappa (B.1.617.1; L452R, E484Q), and the single mutant K417N.
Result: We used a direct ELISA to compare the binding of 2E8 and CB6 to recombinant S1 and RBD proteins, including S1 proteins corresponding to L, alpha, beta, gamma, and epsilon (B.1.429; L452R, E484Q) (Figure 3).
SARS_CoV_2 mutation literature information.
PMID: 
2021
Virology journal
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