Arterial and Venous Thrombosis Complicated in COVID-19: A Retrospective Single Center Analysis in Japan.
PMID: 34869681
2021
Frontiers in cardiovascular medicine
Abstract: SARS-CoV-2 variants of concern/interest (VOC/VOI) carrying the spike protein mutants E484K, N501Y, or L452R were identified by PCR-based analysis.
Method: As the proportion of SARS-CoV-2 variants carrying either N501Y, E484K, and L452R mutations was expanded, RT-PCR was performed using VirSNiP SARS-CoV-2 (TIB Molbiol, Berlin, Germany).
Figure: Based on the results of the epidemiologic analysis in Tokyo, the variant carrying E484K alone, N501Y alone, and L452R alone were considered as R.1 lineage variant, alpha variant, and delta variant, respectively.
Mechanisms of SARS-CoV-2 Evolution Revealing Vaccine-Resistant Mutations in Europe and America.
PMID: 34873910
2021
The journal of physical chemistry letters
3Introduction: Moreover, we have pointed out that Y449S and Y449H are two vaccine-resistant mutations, and ""Y449S, S494P, K417N,
Introduction: Later, we provided a list of most likely vaccine escape RBD mutations with high frequency, including S494P, Q493L, K417N, F490S, F486L, R403K, E484K, L452R, K417T, F490L, E484Q, and A475S.
Analysis of the ARTIC Version 3 and Version 4 SARS-CoV-2 Primers and Their Impact on the Detection of the G142D Amino Acid Substitution in the Spike Protein.
Introduction: Indeed, when we examine 12,441 samples from Houston Methodist patients collected since April of 2021, comparing the occurrence of G142D with L452R (another hallmark Delta substitution in spike), it becomes clear that the G142D uptick is an artifact that corresponds precisely with our adoption of the V4 primers in mid-July 2021.
Figure: (E) Frequency of L452R (gray), D950N (blue), and G142D (orange) amino acid substitutions observed in SARS-CoV-2-positive samples from April through August of 2021.
Figure: L452R, D950N, and G142D are hallmark amino acid substitutions of the Delta variant.
Emergence of novel combinations of SARS-CoV-2 spike receptor binding domain variants in Senegal.
Introduction: All three of the genomes carrying the L452R/N501Y combination belonged to the A.27 lineage (clade 19B) and did not encode the D614G mutation that predominates most global infections today.
Introduction: In addition to strains carrying L452R individually, variant strains carrying a combination of L452R + N501Y (3/117, 2.6%) were also identified.
Introduction: In addition to the L452R + N501Y double mutant, a single genome was identified that carried a unique combination of E484K + N501T spike RBD mutations in a B.1 lineage genome (clade 20C
Additional Positive Electric Residues in the Crucial Spike Glycoprotein S Regions of the New SARS-CoV-2 Variants.
Emergence of Novel SARS-CoV-2 variants in India: second wave.
PMID: 34898481
2021
Journal of infection in developing countries
Abstract: The remaining sequences were assigned to clade 20H, 20J, 20D, 20C, 20G,20E,19A and 19B.The spike mutation frequencies of L452R, E484Q and P681R in Indian state of Maharashtra were 62.4%, 66.5% and 61.5% respectively.
The Delta Variant Mutations in the Receptor Binding Domain of SARS-CoV-2 Show Enhanced Electrostatic Interactions with the ACE2.
Abstract: Recently, a new strain is reported in India that includes a mutation (T478K, and L452R) in the RBD, that is possibly increasing the infection rate.
Introduction: Besides, L452R mutation recently reported to enhance the viral replication by increasing the S protein stability and viral infectivity and viral fusogenicity.
Introduction: In contrary to T478K, the L452R variant is shown to enhance the total binding energy between RBD and ACE2 to be about 2.63 kcal/mol more negative than its value in WT protein.
Introduction: In this research, we study the effect of both of L452R and T478K variants in the
Development of an efficient Sanger sequencing-based assay for detecting SARS-CoV-2 spike mutations.
Abstract: Here, we developed five SARS-CoV-2 spike gene primer pairs (5-SSG primer assay; 69S, 144S, 417S, 484S, and 570S) and verified their ability to detect nine key spike mutations (DeltaH69/V70, T95I, G142D, DeltaY144, K417T/N, L452R, E484K/Q, N501Y, and H655Y) using a Sanger sequencing-based assay.
Loss of Neutralizing Antibody Response to mRNA Vaccination against SARS-CoV-2 Variants: Differing Kinetics and Strong Boosting by Breakthrough Infection.
Introduction: Finally, Delta (B.1.617.2) is responsible for the most recent wave of the COVID-19 pandemic and is characterized by new NTD alterations, together with key RBD mutations (L452R and T478K).
Abstract: In addition to this, SSIPe is used to report the binding affinity between the receptor-binding domain of Spike protein and human ACE2 protein by considering L452R, T478K, E484Q, and N501Y hotspot mutations in that region.
Conclusion: Finally, SSIPe is used to report the binding affinity between the RBD of Spike protein and human ACE2 protein by considering L452R,
Introduction: On the other hand, the variant B.1.617.2 was first identified in India with L452R, T478K, D614G, and P681R mutations in Spike glycoprotein.
SARS_CoV_2 mutation literature information.
PMID: 
2021
Frontiers in cardiovascular medicine
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