Method: Pre-fusion spike protein ectodomain DNA constructs were designed containing the following mutations compared to the Wuhan variant (Wuhan Hu-1; GenBank: MN908947.3): deletion of H69, V70 and Y144, N501Y, A570D, D614G, P681H, T716I, S982A and D1118H in Alpha; L18F, D80A, D215G, L242H, R246I, K417N, E484K, N501Y, D614G and A701V in Beta;
SARS-CoV-2 Mutations and Their Impact on Diagnostics, Therapeutics and Vaccines.
Introduction: In addition, some of the notable substitutions in NTD are R246I (in B.1.351), W152C (in B.1.429), L18F (in B.1.351 and P.1), T19R (in B.1.17 and B.1.617) and G142D (in B.1.617 lineages), all of which are associated with immune-escape.
Table: L18F
Role of the Microbiome in the Pathogenesis of COVID-19.
PMID: 35433495
2022
Frontiers in cellular and infection microbiology
Table: L18F
Emergence and phenotypic characterization of the global SARS-CoV-2 C.1.2 lineage.
Method: The SARS-CoV-2 Wuhan-1 spike, cloned into pCDNA3.1, was mutated using the QuikChange Lightning Site-Directed Mutagenesis kit (Agilent Technologies) and NEBuilder HiFi DNA Assembly Master Mix (NEB) to include D614G (wild-type) or lineage defining mutations for Beta (L18F, D80A, D215G, 241-243del, K417N, E484K, N501Y, D614G and A701V), Delta (T19R, 156-157del, R158G, L452R, T478K, D614G, P681R and D950N
Impact of B.1.617 and RBD SARS-CoV-2 variants on vaccine efficacy: An in-silico approach.
PMID: 35370005
2022
Indian journal of medical microbiology
Discussion: D614G, T20N, D138Y, L18F, R190S, and P26S in the NTD and K417T, E484K and N501Y in the RBD region and H655Y within the furin cleavage site.
Low-frequency variants in mildly symptomatic vaccine breakthrough infections presents a doubled-edged sword.
Abstract: Low-frequency mutations were observed, which have been more recently identified as mutations of interest owing to their location within targeted immune epitopes (P812L) and association with increased replicative capacity (L18F).
E-Volve: understanding the impact of mutations in SARS-CoV-2 variants spike protein on antibodies and ACE2 affinity through patterns of chemical interactions at protein interfaces.
Introduction: It presents the following mutations: L18F, T20N, P26S, D138Y, R190S, K417T, E484K, N501Y, H655Y, and T1027I.
Introduction: The Beta variant has non-synonymous spike mutations such as LAL 242-244 deletion, D80A, D215G, E484K, N501Y, A701V, L18F, R246I, K417N, and D614G.
Neutralisation Hierarchy of SARS-CoV-2 Variants of Concern Using Standardised, Quantitative Neutralisation Assays Reveals a Correlation With Disease Severity; Towards Deciphering Protective Antibody Thresholds.
Conformational Flexibility and Local Frustration in the Functional States of the SARS-CoV-2 Spike B.1.1.7 and B.1.351 Variants: Mutation-Induced Allosteric Modulation Mechanism of Functional Dynamics and Protein Stability.
PMID: 35163572
2022
International journal of molecular sciences
Result: At the same time, structural maps for the S-B.1.351 conformations (Figure 6E,F) highlighted the role of the NTD sites (L18F, D80A, D215G) and especially RBD sites (K417N, E484K, N501Y) that belong to the moving regions in slow motions.
Result: Mutational scanning maps for variant positions in the S-B.1.351 conformations showed similar and moderate stabilization changes for the NTD variant L18F, D80A, and D215G.
Result: The increased mobility of mutational sites is particularly apparent in the open state of the S-B.1.351 variant (Figure 3F), where all the modified positions L18F,
AstraZeneca COVID-19 vaccine induces robust broadly cross-reactive antibody responses in Malawian adults previously infected with SARS-CoV-2.
Introduction: The beta variant bears genetic changes in the functional domain of the SARS-CoV-2 spike (S) protein including substitutions in the receptor-binding domain (RBD) (E484K, N501Y and K417N), four substitutions and a deletion in N-terminal domain (NTD) (L18F, D80A, D215G, L242H and R246I) and substitutions in S2 (D614G and A701V) regions.
SARS_CoV_2 mutation literature information.
PMID: 
2022
Scientific reports
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