Introduction: The Marseille-484K.V1, or R.1, lineage is another example of convergent evolution leading to the amino acid substitution E484K, as also observed for the substitutions L452R, N501Y, Q677H, and L18F, which have occurred independently in various SARS-CoV-2 lineages and geographical areas.
Mutation profile of SARS-CoV-2 genome in a sample from the first year of the pandemic in Colombia.
PMID: 34933126
2022
Infection, genetics and evolution
Abstract: In the latter, we identified mutations E484K, L18F, and D614G.
Result: The mutations p.Lys1191Asp (K1191D), p.Glu484Lys (E484K), p.Leu18Phe (L18F), p. Table: L18F
Table: p.Leu18Phe
Discussion: According to several studies, the L18F mutation, also located in the S glycoprotein and present in 16 of our samples, apparently gives a replicative advantage to the Alpha, Beta, and Gamma lineages.
Genomic characterization of the dominating Beta, V2 variant carrying vaccinated (Oxford-AstraZeneca) and nonvaccinated COVID-19 patient samples in Bangladesh: A metagenomics and whole-genome approach.
Abstract: Noteworthily, the receptor binding domain (RBD) region of S protein of all the isolates harbored similar substitutions including K417N, E484K, and N501Y that signify the Beta, while D614G, D215G, D80A, A67V, L18F, and A701V substitutions were commonly found in the non-RBD region of Spike proteins.
Emergence of two distinct variants of SARS-CoV-2 and an explosive second wave of COVID-19: the experience of a tertiary care hospital in Pune, India.
Result: Two sequences from 2021 (CD211295 [MW969753] and CD210761 [MZ021503]) belonging to the original prevalent lineage B.1.1.306 had four character
Table: L18F
Discussion: Interestingly, two Indian strains (CD211295-MW969753, CD210761-MZ021503) had a combination of two substitutions, L18F and E484K.
Discussion: It was shown that the proportion of genomes with the L18F mutation increased from 35% in September to 43% in October and to 52% in November 2020, suggesting that this mutation facilitates virus transmission.
Discussion: The L18F substitution was dominant in England before the emergence of the UK strain.
Discussion: The role of simultaneous L18F and E484K mutations in virus transmission needs to be evaluated.
A short plus long-amplicon based sequencing approach improves genomic coverage and variant detection in the SARS-CoV-2 genome.
Method: To construct the expression plasmids for the S protein of Beta, a construction of trimeric S of the Wuhan strain was used as the template and nine pairs of primers of S (L18F forward primer 5'-GAGCAGCCAGTGCGTGAATTTCACCACCAGAACCCAGCTG-3', L18F reverse primer 5'-CAGCTGGGTTCTGGTGGTGAAATTCACGCACTGGCTGCTC -3'; D80A forward primer 5'-GCACCAAGAGATTCGCCAATCCTGTGCTGCC-3' and D80A reverse primer 5'-GGCAGCACAGGATTGGCGAATCTCTTGGTGC-3'; D215G for
Result: Of the point mutations in the NTD (L18F, D80A, D215G, and R246I) only L18F is part of the epitope.
Whole-Genome Sequencing of SARS-CoV-2 Strains from Asymptomatic Individuals in India.
SARS_CoV_2 mutation literature information.
PMID: 
2022
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