In silico investigations of heparin binding to SARS-CoV-2 variants with a focus at the RBD/ACE2 interface.
PMID: 35194375
2022
Process biochemistry (Barking, London, England)
Introduction: The variant, B.1.351 and B.1.1.28 carries 9 and 11 mutations in the spike (S) protein, respectively, including three mutations in the receptor binding domain (RBD), K417N/T, E484K, and N501Y.
Result: 2F) and K417T-E484K-N501Y.
Result: A total of 8 H-bonds with 136 non-bonded contacts were observed in K417T-E484K-N501Y variant.
Result: As can be seen, the Es for WT RBD-heparin complex was - 129.55 kJ/mol which was much higher than L452
Evolutionary history of the SARS-CoV-2 Gamma variant of concern (P.1): a perfect storm.
PMID: 35266951
2022
Genetics and molecular biology
Introduction: Currently, the WHO has identified the Gamma lineage (B.1.1.28.1, P.1 or Gamma; Nextstrain clade 20J/V3) with the following key S mutations: L18F, T20N, P26S, D138Y, R190S, K417T, E484K, N501Y, D614G, H655Y, T1027I, and V1176F.
Table: K417T
Discussion: VOCs Beta and Gamma share an additional RDB mutation, K417N/T , while UK researchers detected cases of Alpha lineages with the 484K allele, which also characterizes the Beta and Gamma lineage
Insights into the structure and dynamics of SARS-CoV-2 spike glycoprotein double mutant L452R-E484Q.
Introduction: Another variant, B.1.351 (Mwenda et al.) and P.1 variant (Francisco et al.) carries 9 and 11 spike protein mutations, respectively, including 3 mutations in the receptor-binding domain (RBD), K417N/T, E484K, and N501Y.
Identifying SARS-CoV-2 Variants of Concern through saliva-based RT-qPCR by targeting recurrent mutation sites.
Introduction: K417T and K417N SNPs and many substitutions at E484 also reduce viral susceptibility to neutralizing antibodies.
Introduction: We also evaluated the efficacy of TaqPath assays for K417T, E484K, E484Q, and L452R in saliva.
Introduction: We chose to evaluate assays for biochemically significant mutations that also provide differential strain typing for SARS-CoV-2 VOCs, namely SDelta69-70, ORF1aDelta3675-3677, K417T, E484K, E484Q, and L452R.
Method: SARS-CoV-2 TaqMan Assays for S substitutions K417T, E484K, <
SARS-CoV-2 Beta and Delta variants trigger Fc effector function with increased cross-reactivity.
Method: The SARS-CoV-2 Wuhan-1 spike, cloned into pCDNA3.1 was mutated using the QuikChange Lightning Site-Directed Mutagenesis kit (Agilent Technologies) and NEBuilder HiFi DNA Assembly Master Mix (NEB) to include D614G (original) or lineage defining mutations for Alpha (DEL69-70, DEL144, N501Y, A570D, D614G, P681H, T716I, S982A, D1118H), Beta (L18F, D80A, D215G, 242-244del, K417N, E484K, N501Y,
Table: K417T
Characterization of SARS-CoV-2 Variants N501Y.V1 and N501Y.V2 Spike on Viral Infectivity.
PMID: 34722330
2021
Frontiers in cellular and infection microbiology
Introduction: Interestingly, N501Y.V3 (also known as P.1) lineage in Brazil almost has the same three RBD mutations as N501Y.V2 lineage, except for the K417N/T substitution.
Discussion: Indeed, N501Y.V3 lineage includes three mutations in the RBD region (K417T, E484K and N501Y), and it almost has the same three mutations present in RBD as N501Y.V2 lineage, except for K417N/T substitution.
Insights on the SARS-CoV-2 genome variability: the lesson learned in Brazil and its impacts on the future of pandemics.
Introduction: Among them, the variants P.1 and P.2, first isolated in Brazil possess substitutions of interest in the S protein, including K417T, E484K, N501Y, D614G, and H655Y.
Epidemiology of COVID-19: An updated review.
PMID: 34759999
2021
Journal of research in medical sciences
Method: They are: Result: The B.1.351 control showed mutation in E484K, D614G, A701V, K417N, N501Y, and L242_244L, B.1.1.7 showed mutations in D614G, delH69V70, N501Y, and Q27stop, the control B.1.617.1 showed mutation in E484Q, P681R, L452R, D614G, and P.1 showed mutations in E484K, D614G, K417T, N501Y, and T20N.
Table: K417T
Cross-Neutralizing Activity Against SARS-CoV-2 Variants in COVID-19 Patients: Comparison of 4 Waves of the Pandemic in Japan.
PMID: 34631915
2021
Open forum infectious diseases
Introduction: It bears 12 mutations in the spike gene, including K417T, E484K, and N501Y, which are the same 3 amino acid substitutions found in B.1.351.
Discussion: On the other hand, because P.1 and B.1.351 have similar mutations in their RBD (including E484K, K417T/N, and N501Y), it might be thought that the neutralization of both variants would be affected similarly.
SARS_CoV_2 mutation literature information.
PMID: 
2022
Process biochemistry (Barking, London, England)
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