Method: Primers used for the construction of the RBD-mFc proteins for the different variants (Alpha RBD-mFc, containing mutation N501Y; Gamma RBD-mFc containing mutations K417T, E484K, and N501Y; Delta RBD-mFc containing mutations L452R and T478K); Epsilon RBD-mFc containing mutation L452R; and Kappa RBD-mFc containing mutations L452R and E484Q), were obtained from Sigma-Aldrich and are shown in Supplementary Table 2 .
Early Genomic, Epidemiological, and Clinical Description of the SARS-CoV-2 Omicron Variant in Mexico City.
Result: K417N/T is present in the Alpha and Gamma variants, and both mutations facilitate immune escape for monoclonal antibodies (bamlanivimab/LY-CoV555), escape from neutralization by convalescent plasma, and escape by sera from BNT162b2-vaccinated individuals.
Antigenicity comparison of SARS-CoV-2 Omicron sublineages with other variants contained multiple mutations in RBD.
Abstract: Our results indicate that mutations E484K and K417T compensate each other in terms of binding affinity, while the mutation N501Y promotes a more convoluted effect.
Abstract: This variant contains the N501Y, E484K and K417T mutations in the receptor binding domain (
Introduction: The gamma variant carries the K417T, E484K and N501Y mutations previously described in RBM.
Introduction: While the K417T mutation results in the loss of a salt bridge with D30, mutation E484K increases the electrostatic complementarity of the binding partners.
Role of the Microbiome in the Pathogenesis of COVID-19.
PMID: 35433495
2022
Frontiers in cellular and infection microbiology
Table: K417T
Inhibitor screening using microarray identifies the high capacity of neutralizing antibodies to Spike variants in SARS-CoV-2 infection and vaccination.
Introduction: The B.1.351 and P.1 variants contain three RBD mutations at E484K, N501Y, and K417N or K417T, respectively.
Impact of B.1.617 and RBD SARS-CoV-2 variants on vaccine efficacy: An in-silico approach.
PMID: 35370005
2022
Indian journal of medical microbiology
Discussion: D614G, T20N, D138Y, L18F, R190S, and P26S in the NTD and K417T, E484K and N501Y in the RBD region and H655Y within the furin cleavage site.
E-Volve: understanding the impact of mutations in SARS-CoV-2 variants spike protein on antibodies and ACE2 affinity through patterns of chemical interactions at protein interfaces.
Abstract: Discussion: Molecular dynamics simulations followed by Poisson-Boltzmann calculations corroborate the higher complementarity to the receptor and lower to the antibodies for the K417T/E484K/N501Y (Gamma) mutant compared to the wild-type strain, as pointed by E-Volve, as well as an intensification of this effect by changes at the protein conformational equilibrium in solution.
Introduction: As in cases of reinfection, by the non-synonymous convergent
Method: A Gamma lineage RBD (with mutations E484K, N501Y, and K417T) was modelled comparatively using SWISS-MODEL.
Figure: 1 (K417T, E484K, and N501Y).
Potential inhibitor for blocking binding between ACE2 and SARS-CoV-2 spike protein with mutations.
Result: After treatment of the cells with the indicated concentration of GB-1, the number of 293 T cells with high binding to the RBD with K417T-E484K-N501Y mutation was decreased in both ACE2-positive cells and the top population in the 200-300 mug/mL GB-1 treatment group .
Result: Effect of GB-1 on the binding between ACE2 and RBD with K417T-E484K-N501Y mutation.
Result: Next, we investigated the effect of GB-1 on the binding between ACE2 and RBD with K417T-E484K-N501Y mutation through dual-color flow cytometric analysis.
Result: The number of 293 T cells with low binding to the
Multiple SARS-CoV-2 Variants Exhibit Variable Target Cell Infectivity and Ability to Evade Antibody Neutralization.
SARS_CoV_2 mutation literature information.
PMID: 
2022
Frontiers in immunology
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