literature

Comparative Analysis of Five Multiplex RT-PCR Assays in the Screening of SARS-CoV-2 Variants.

PMID: 35208761 2022 Microorganisms

Abstract: Then, specimens were screened for the detection of L452R, W152C, K417T, K417N, E484Q, E484K and N501Y mutations using the SARS-CoV-2 Variants II Assay Allplex, UltraGene Assay SARS-CoV-2 452R & 484K & 484Q Mutations V1, COVID-19 Ultra Variant Catcher, SARS-CoV-2 Extended ELITe MGB and Simplexa SARS-CoV-2 Variants Direct.

Discussion: The analyzed real-time RT-PCR assays target three or four of the following amino acid variations: L452R, W152C, K417T, K417N, E484Q, E484K and

PMID: 35222380 2022 Frontiers in immunology

Introduction: Few mutations include K417T/N, E484K, L452R, N501Y, P681R, D614G, etc.

Introduction: Several mutations have been observed among VOI, and some common mutations are K417T/N, E484K, L452R, N501Y, P681R and Introduction: developed pseudoviruses with different RBD mutations or other parts of S-glycoprotein such as N501Y, K417N/T, and E484K, to illustrate the neutralization event.

A screening strategy for identifying the dominant variant of SARS-COV-2 in the fifth peak of Kurdistan- Iran population using HRM and Probe-based RT-PCR assay.

PMID: 35271889 2022 Journal of virological methods

Table: K417T

Allosteric Determinants of the SARS-CoV-2 Spike Protein Binding with Nanobodies: Examining Mechanisms of Mutational Escape and Sensitivity of the Omicron Variant.

PMID: 35216287 2022 International journal of molecular sciences

Introduction: Neutralization of SARS-CoV-2 by low-picomolar and mutation-tolerant VHH nanobodies that bind synergistically to the opposite sides of the RBD produced a binding avidity effect unaffected by immune-escape mutants K417N/T, E484K, N501Y, and L452R.

Genomic Diversity of SARS-CoV-2 in Algeria and North African Countries: What We Know So Far and What We Expect?

PMID: 35208920 2022 Microorganisms

Discussion: Comparative genomic analysis of SARS-CoV-2 genomes revealed multiple crucial mutations to the Spike gene including K417N, K417T, E484K, N501Y, A570D, D614G, P681H, T716I, S982A and D1118H, which may aggravate the severity of SARS-CoV-2 more than the wild type variant, and potentially raise the concern of vaccine efficacy against novel strains.

Emergency SARS-CoV-2 Variants of Concern: Novel Multiplex Real-Time RT-PCR Assay for Rapid Detection and Surveillance.

PMID: 35196812 2022 Microbiology spectrum

Abstract: This multiplex PCR typing method was established to detect 9 mutations with specific primers and probes (DeltaHV 69/70, K417T, K417N, L452R, E484K, E484Q, N501Y, P681H, and P681R) against the receptor-binding domain of the spike

Table: K417T

Figure: (A) VOI (K417T and P681H) and VOC (DeltaHV69-79, K417N, L452R, E484K, E484Q, N501Y, and P681R).

Misidentification of the SARS-CoV-2 Mu variant using commercial mutation screening assays.

PMID: 35194675 2022 Archives of virology

Introduction: Any other mutation combination or the absence of any mutations required the use of a second rRT-PCR mutation assay, Allplex SARS-CoV-2 Variants II Assay (Seegene, Korea), which screens for the mutations K417N, K417T, L452R, and W152C.

Introduction: Following the scheme described above, in August 2021, we detected three strains that presented K417N, N501Y, and E484K mutations but did not present delH69/V70, W152C, K417T, L452R, or V1176F mutations.

Spread of Gamma (P.1) Sub-Lineages Carrying Spike Mutations Close to the Furin Cleavage Site and Deletions in the N-Terminal Domain Drives Ongoing Transmission of SARS-CoV-2 in Amazonas, Brazil.

PMID: 35196783 2022 Microbiology spectrum

Discussion: One of the most divergent Gamma sequences detected in Brazil was a P.1.4 lineage variant sampled in the Amazonas in October 2021 (EPI_ISL_5621224) that harbors 20 S mutations (eight more than the parental P.1 lineage), including several in common with (Delta144, Q498R, N501Y, H655Y, and N679K) or like (T95N, K417T, and E484K) those detected in Omicron.

SARS-CoV-2 Omicron Spike recognition by plasma from individuals receiving BNT162b2 mRNA vaccination with a 16-week interval between doses.

PMID: 35216664 2022 Cell reports

Introduction: The accumulation of E484K and K417N/T mutations along with N501Y in the receptor binding domain (RBD) led to the emergence of Beta (B.1.351) and Gamma (P.1) lineages, which rapidly spread worldwide.

Classical and Next-Generation Vaccine Platforms to SARS-CoV-2: Biotechnological Strategies and Genomic Variants.

PMID: 35206580 2022 International journal of environmental research and public health

Introduction: The E484K, K417T/N, and N501Y genomic variants are the most worrisome because they generate greater transmissibility by increasing viral contagion.