Result: K417N/T is present in the Alpha and Gamma variants, and both mutations facilitate immune escape for monoclonal antibodies (bamlanivimab/LY-CoV555), escape from neutralization by convalescent plasma, and escape by sera from BNT162b2-vaccinated individuals.
Result: Figure 3 shows the most frequent amino acid substitutions in the RBD (G339D, R346K, K417N, S371L, S373P, S375F, N440K, Q498R, and N501Y).
Result: We found that the substitutions R346K, K417N, and N440K were more preval
A SARS-CoV-2 Wuhan spike virosome vaccine induces superior neutralization breadth compared to one using the Beta spike.
Introduction: However, neutralization of the Wuhan strain was reduced when a variant vaccine was used that contained the K417N, E484K, N501Y and D614G mutations, which agrees well with our results.
Method: Pre-fusion spike protein ectodomain DNA constructs were designed containing the following mutations compared to the Wuhan variant (Wuhan Hu-1; GenBank: MN908947.3): deletion of H69, V70 and Y144, N501Y, A570D, D614G, P681H, T716I, S982A and D1118H in Alpha; L18F, D80A, D21
SARS-CoV-2 Mutations and Their Impact on Diagnostics, Therapeutics and Vaccines.
Introduction: And cilgavimab had lower activity against N501Y+D614G mutants, including B.1.429 (carrying L452R), B.1.617.2 (carrying L452R + T478K) and B.1.351 (carrying K417N + E484K + N501Y).
Introduction: However, in-vitro neutralization assays show that casirivimab alone (without imdevimab) had reduced activity against (i) K417N+ E484K substitutions found in P.1 lineage (Brazil variant), and (ii) E484Q mutation expressed in B.1.617.1/B.1.617.3 lineages (Delta/Indian variants) (FDA).
Introduction: However, it showed a significant reduction in the neutralization of B.1.351 (carrying PMID: 35279013
2022
Biomedicine & pharmacotherapy
Discussion: Beta variant contains nine mutations in SARS-CoV-2 spike protein: D614G, Delta242-Delta244, R246I, K417N, E484K, N501Y, and A701V.
Discussion: However, 50 mug/mL glycyrrhizic acid did not affect the binding between ACE2 and RBD with K417N-E484K-N501Y mutation.
Discussion: In conclusion, our results suggest that GB-1 could be a candidate for prophylaxis against some variants of SARS-CoV-2 infection through the inhibition of the binding between ACE2 and RBD with mutations (N501Y
Cross-Neutralizing Breadth and Longevity Against SARS-CoV-2 Variants After Infections.
Introduction: B.1.351, which was found in South Africa, has three mutations (K417N, E484K, and N501Y) in the RBD.
Introduction: Finally, B.1.1.529, which was detected in Botswana on November 11, 2021 and South Africa on November 14, 2021, has 15 mutations (G339D, S371L, S373P, S375F, K417N, N440K, G446S, S477N, T478K, E484A, Q493R, G496S, Q498R, N501Y, and
Analysis of SARS-CoV-2 variants B.1.617: host tropism, proteolytic activation, cell-cell fusion, and neutralization sensitivity.
Result: Notably, similar as K417N, N501Y, and E484K in the B.1.351 variant, RBD mutations in the B.1.617 variants also showed enhanced infectivity in mouse cells.
Targeted Sanger sequencing to recover key mutations in SARS-CoV-2 variant genome assemblies produced by next-generation sequencing.
Abstract: We applied the primer set and wet-laboratory protocol to sequence 222 samples that were missing positions with key mutations K417N, E484K, and N501Y due to poor coverage after NGS sequencing.
Abstract: We successfully sequenced 153 samples of 222 (69 %) using Sanger sequencing and confirmed the occurrence of key beta variant mutations (K417N, E484K, N501Y) in the S genes of 142 of 153 (93 %) samples.
Neutralisation sensitivity of the SARS-CoV-2 omicron (B.1.1.529) variant: a cross-sectional study.
PMID: 35305699
2022
The Lancet. Infectious diseases
Introduction: Deep mutational scanning data suggest that E484A and K417N, in addition to G446S and Q493R (which are not present in other variants of concern) are the largest contributors to the resistance profile of the omicron variant.
Interaction Analysis of the Spike Protein of Delta and Omicron Variants of SARS-CoV-2 with hACE2 and Eight Monoclonal Antibodies Using the Fragment Molecular Orbital Method.
PMID: 35312321
2022
Journal of chemical information and modeling
Method: The omicron variant has 15 mutations in the RBD domain of the S protein: G339D, S371L, S373P, S375F, K417N, N440K, G446S, S477N, T478K, E484A, Q493K/R, G496S, Q498R, N501Y, and Y505H.
Result: The G339D, S373P, K417N, G446S,
The SARS-CoV-2 Delta variant induces an antibody response largely focused on class 1 and 2 antibody epitopes.
5Result: Notably, sublineages of the Delta variant, AY.1 and AY.2, colloquially referred to as ""Delta+"" lineages, contain the K417N mutation."
Result: K417N has a larger effect on neutralization in the Delta than in the D614G spike.
Result: Delta + E484K has an ~8-fold effect on neutralization for primary Delta-infection elicited antibodies, but only a ~3-fold effect for mRNA vaccination- or Delta breakthrough infection-elicited antibodies, comparable to the effect of Delta + K417N mutation.
Result: The Delta + K417N mutation resulted in a ~3-fold reduction in neutralization for 2x BNT162b2 and Delta breakthrough plasmas when compared to the Delta spike.
SARS_CoV_2 mutation literature information.
PMID: 
2022
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