Abstract: Here we perform a detailed affinity and kinetics analysis of the effect of five common RBD mutations (K417N, K417T, N501Y, E484K, and S477N) and two common ACE2 mutations (S19P and K26R) on the RBD/ACE2 interaction.
Abstract: Taken together with other s
Introduction: Although the K417N/T mutations found in the South African (B.1.351) and Brazilian (P.1) variants both decreased the affinity, the affinity-enhancing N501Y and E484K mutations that are also present in both variants confer a net ~4-fold increase in the affinity of their RBDs for ACE2.
Community-level SARS-CoV-2 sequence diversity revealed by wastewater sampling.
PMID: 34438144
2021
The Science of the total environment
Table: K417N
Molecular Dynamics Simulation Study of the Interaction between Human Angiotensin Converting Enzyme 2 and Spike Protein Receptor Binding Domain of the SARS-CoV-2 B.1.617 Variant.
Introduction: The South African variant B.1.351 or 20H/501Y is characterized by three mutations in the RBD region of the S protein:K417N, E484K, and N501Y.
Impact of Full Vaccination with mRNA BNT162b2 on SARS-CoV-2 Infection: Genomic and Subgenomic Viral RNAs Detection in Nasopharyngeal Swab and Saliva of Health Care Workers.
Method: Moreover, the RT-PCR method was used for the analysis of known viral mutations using the REALQUALITY SARS-CoV-2 Variants (AB ANALITICA, Padova, Italy) (targeting N501Y, K417T and K417N) and SARS-CoV-2 Nucleic Acid Mutation Diagnostic kit (Sansure Biotech Inc, Hunan, China) (targeting N501Y and HV69/70del), following the manufacturers' instructions.
Result: The analysis was performed using two commercial kits (REALQUALITY SARS-CoV-2 Variants -AB ANALITICA, Padova, Italy- targeting N501Y, K417T and K417N and SARS-CoV-2 Nucleic Acid Mutation Diagnostic kit-Sansure Biotech Inc., Hunan, China- targeting N501Y and 69/70del) in order to combine the targeted mutations.
Result: Viral mutations ( PMID: 34449757
2021
Tropical medicine and infectious disease
Method: SGTF-negative samples were screened for the presence of notable types of spike protein mutations (HV 69-70 deletion, N501Y, K417N, E484K, and K417T) using a commercial multiplex real-time PCR kit (Seegene Allplex SARS-CoV-2 Variants I Assay, Arrows Diagnostics, Genova, Italy).
Predominance of the SARS-CoV-2 Lineage P.1 and Its Sublineage P.1.2 in Patients from the Metropolitan Region of Porto Alegre, Southern Brazil in March 2021.
Discussion: B.1.351 and P.1 share a mutation in the K417 site (K417N and K417T, respectively) and the E484K replacement, which is also observed in the P.2 lineage.
Myxobacterial depsipeptide chondramides interrupt SARS-CoV-2 entry by targeting its broad, cell tropic spike protein.
PMID: 34463219
2021
Journal of biomolecular structure & dynamics
Result: After determining the binding affinities of the chondramides towards point-mutated SARS-CoV-2 spike variants, the selected compounds were subjected to molecular docking against recently known strains with more than one amino acid substitutions in the spike RBD sequence namely, the South African variant (Wibmer et al.,) (N501Y-E484K-K417N) and the Brazilian variant (Nonaka et al., 2021) (N501Y-E484K-K417T).
Result: Similarly, the South African variant is characterized by two additional mutations in the RBD namely N501Y and K417N (Wibmer et al.,).
Figure:
A vaccine-induced public antibody protects against SARS-CoV-2 and emerging variants.
Result: We evaluated the prophylactic efficacy of 2C08 against the D614G strain, a fully infectious recombinant chimeric SARS-CoV-2 with the B.1.351 S gene in the WA1/2020 backbone (Wash-B.1.351; D80A, 242-244 deletion, R246I, K417N, E484K, N501Y, D614G and A701V), and the B.1.617.2 strain in 5-to-6-week-old male Syrian hamsters.
Evolution, Mode of Transmission, and Mutational Landscape of Newly Emerging SARS-CoV-2 Variants.
Result: Furthermore, we analyzed the K417N mutation.
Result: Here, the structure of K417N is changed due to the replacement Lys417 Asn.
Result: Some significant mutations (E484K, K417T/N, N501Y, and D614G) found in emerging variants and their structural landscapes.
Result: The structural analysis of K417N is shown in different forms, such as interaction abilities of the wild-type residues.
Result: These are present in spike receptor binding domain (RBD) (E484K, K417T/N, and
SARS-CoV-2 Spike Protein Mutations and Escape from Antibodies: A Computational Model of Epitope Loss in Variants of Concern.
PMID: 34468141
2021
Journal of chemical information and modeling
Introduction: All such sequences contain various mutations due to nonsynonymous nucleotide changes in the receptor-binding domain (RBD), including E484K, N501Y, and/or K417N.
SARS_CoV_2 mutation literature information.
PMID: 
2021
eLife
Browser Board
Co-occurred Entities
Filtrator
ViMRT