Abstract: Furthermore, we showed that RBD co-mutations K417N, E484K, and N501Y present in B.1.351 appear more resistant to NAbs and human convalescent plasma from the early stage of the pandemic, possibly due to an additive effect.
Abstract: The comprehensive escape mutation map not only confirms th
Introduction: Among the SARS-CoV-2 variants of concern, Beta B.1.351 (RBD-K417N/E484K/N501Y) discovered in South Africa and Gamma P.1 (RBD-K417T/E484K/N501Y) discovered in Brazil have been demonstrated to have high potential to reduce the efficacy of some vaccines.
Spike protein evolution in the SARS-CoV-2 Delta variant of concern: a case series from Northern Lombardy.
Discussion: AY.2 additionally harbours V70F (unique among delta lineages), A222V (shared with AY.9, AY.10, AY.11, AY.19 and AY.24) and K417N (as for AY.1) K356N and V1228L have also been rarely reported.
Emergence in southern France of a new SARS-CoV-2 variant harbouring both N501Y and E484K substitutions in the spike protein.
Result: Sequence alignment of RBD from SARS-CoV-2, B.1.1.7, and B.1.351 variants spike proteins are (the N501Y, K417N, and E484K mutations) shown in red with circle.
The evaluation of potential global impact of the N501Y mutation in SARS-COV-2 positive patients.
Introduction: On the other hand, in B.1.351 lineage, the K417N, and E484K mutations are found in the RBD region.
In Vitro Effect of Taraxacum officinale Leaf Aqueous Extract on the Interaction between ACE2 Cell Surface Receptor and SARS-CoV-2 Spike Protein D614 and Four Mutants.
Introduction: Free energy perturbation calculations for interactions of the N501Y and K417N mutations with both the ACE2 receptor and an antibody derived from COVID-19 patients raise important questions about the possible human immune response and the success of already-available vaccines.
Introduction: Here we report on the inhibitory potential of dandelion on the binding of the spike S1 protein RBD to the hACE2 cell surface receptor and compare the effect of the original D614 spike protein to its D614G, N501Y, and mix (K417N, E484K, and N501Y) mutations.
Introduction: The Beta variant contai
Prior infection with SARS-CoV-2 boosts and broadens Ad26.COV2.S immunogenicity in a variant-dependent manner.
Method: SARS-CoV-2 pseudotyped lentiviruses were prepared by co-transfecting the HEK293T cell line with either the SARS-CoV-2 ancestral variant spike (D614G), the Beta spike (L18F, D80A, D215G, K417N, E484K, N501Y, D614G, A701V, 242-244 del) or the Delta spike (T19R, R158G L452R, T478K, D614G, P681R, Table: K417N
Emerging SARS-CoV-2 variants expand species tropism to murines.
Result: 1e-g), suggesting the K417N/T and E484K mutation in the RBD of these variants might further facilitate mouse adaptation in addition to the N501Y substitution.
Discussion: Apart from the N501Y mutation, several amino acid substitutions including K417N, E484K, Q493H/K, and Q498H were also suggested to be critical for SARS-CoV-2 adaptation in murine species.
Discussion: This is congruous with our observation that B.1.351 replicated to a higher level than B.1.1.7 and P.3 in wildtype mice since B.1.351 carries K417N, E484K in addition to N501Y.
Functional Effects of Receptor-Binding Domain Mutations of SARS-CoV-2 B.1.351 and P.1 Variants.
Result: The K417N ( Figure 4A ) and K417T mutants ( Figure 4B ) had no noticeable effect.
Result: The K417N:but not the K417T:appeared to be inhibited better than the wt.
Result: We observed a 1.9- and 1.5-fold reduction in the median inhibition of the E484K and N501Y RBD compared to the wt, while the substitutions in the 417 position had puzzlingly opposite effects with apparent 1.4- and 1.2-fold inhibition gain for K417N and K417T, respectively (Friedman test p < 0.0001 for all).
Result: When comparing the inflection temperatures (Ti) of the single RBD mutations with their wt counterpart, we observed a destabilizing
Global Prevalence of Adaptive and Prolonged Infections' Mutations in the Receptor-Binding Domain of the SARS-CoV-2 Spike Protein.
Result: Additionally, mutations K417N, K417T, T470N, T478R, E484A, F490S and Q493K were observed.
Result: In our study, the highest prevalence of the K417N mutation was found in Finland (2/13 sequences), followed by the Philippines (8/71 sequences).
Result: The K417N/T mutation has been shown to have impaired ACE2 binding.
Result: The K417N mutation was first identified in South Africa (beta variant), while the K417T mutation was first identified in Brazil (gamma variant).
Evolution of SARS-CoV-2 Key Mutations in Vienna Detected by Large Scale Screening Program.
SARS_CoV_2 mutation literature information.
PMID: 
2021
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