Antibody-dependent cellular cytotoxicity response to SARS-CoV-2 in COVID-19 patients.
PMID: 34561414
2021
Signal transduction and targeted therapy
Discussion: The K417N+E484K+N501Y triple mutant in the B.1.351 variant significantly reduces the neutralizing activity of convalescent and post-vaccination sera.
E484K mutation in SARS-CoV-2 RBD enhances binding affinity with hACE2 but reduces interactions with neutralizing antibodies and nanobodies: Binding free energy calculation studies.
PMID: 34562851
2021
Journal of molecular graphics & modelling
Introduction: In the 501Y.V2 and 501Y.V3 variants, besides the N501Y mutation, two other mutations E484K and K417N (or K417T) occurred in RBD of the S protein, and it was revealed that the E484K mutation obviously enhances the binding affinity between RBD and hACE2.
Mutations of SARS-CoV-2 RBD May Alter Its Molecular Structure to Improve Its Infection Efficiency.
Introduction: However, there have been several mutations reported in SARS-CoV-2 RBD, such as N501Y, L452R, S477N, E484K, A502S, N439K, S494P, T478K, K417N, and K417T.
Clinical Characterization and Genomic Analysis of Samples from COVID-19 Breakthrough Infections during the Second Wave among the Various States of India.
Discussion: K417N, E484K, L452R, and E484Q are the mutations known to disrupt receptor-binding domain (RBD) binding capacity, making them more infectious by immune escape against the current vaccines.
Discussion: Delta AY.1 and AY.2 are characterized by the presence of the K417N mutation in the spike protein region.
Characterization and structural basis of a lethal mouse-adapted SARS-CoV-2.
Discussion: Except for N501Y and K417N, several other amino acid substitutions in MASCp36 were also found in human variants, including L37F in nsp6 and D128Y in N protein.
Discussion: For example, the 501Y.V1 variant firstly detected in the United Kingdom contained the unique N501Y mutation in RBD, and more recent SARS-CoV-2 variants (501Y.V2 and 501Y.V2) contained both N501Y and K417N substitutions in S protein.
Discussion: More importantly, Cryo-EM structures of both hACE2 and mACE2 in complex with RBDMASCp25 and RBDMASCp36 define preciously the atomic determinants of the receptor-binding switch:
Discussion: In contrast, the Beta variant is comprised of several restrictive mutations ( 242-244, K417N, and E484K) and only one mutation that modestly increased fusion (D215G).
Discussion: The E484K and K417N RBD mutations in the Beta variant may also increase ACE2 affinity, particularly when in conjunction with N501Y (preprint: Nelson et al,; Zahradnik et al,).
Discussion: The K417N substitution, and to a lesser degree 242-244, had a lower affinity to ACE2 and also restricted cell-cell fusion.
Discussion: Upon examining the potential of S proteins carrying individual mutations to bind to human monoclonal antibodies, we found that the ones that restric
A Combination Adjuvant for the Induction of Potent Antiviral Immune Responses for a Recombinant SARS-CoV-2 Protein Vaccine.
Introduction: This reduction is especially prominent with the B.1.351 variant, which contains the N501Y mutation shared by the B.1.1.7 and P.1 variants, and two additional mutations (K417N, E484K) in the spike receptor binding domain (RBD).
Variants of SARS-CoV-2, their effects on infection, transmission and neutralization by vaccine-induced antibodies.
PMID: 34604978
2021
European review for medical and pharmacological sciences
Abstract: S477N, E484K, Q677H, E484Q, L452R, K417T, K417N and N501Y.
Abstract: Deadly K417N+E484K+N501Y triplet mutations found in B.1.351 and P.1 have increased the transmission ability of these strains by 50% leading to greater COVID-19 hospitalization, ICU admissions and deaths.
Rapid and High-Throughput Reverse Transcriptase Quantitative PCR (RT-qPCR) Assay for Identification and Differentiation between SARS-CoV-2 Variants B.1.1.7 and B.1.351.
Method: This enabled the first identification of the B.1.1.7-related mutations 69-70Del, 144Del, N501Y, S982A, and D1118H and B.1.351-related mutations D215G, 242Del K417N, N501Y, and E484K.
Result: Out of 20 suspected samples, four contained the following mutations in the spike gene: D215G, the 242 to 244 deletion, K417N, E484K, and N501Y, all associated with the B.1.351 variant.
SARS_CoV_2 mutation literature information.
PMID: 
2021
Journal of virology
Browser Board
Co-occurred Entities
Filtrator
ViMRT