Inhibitor screening using microarray identifies the high capacity of neutralizing antibodies to Spike variants in SARS-CoV-2 infection and vaccination.
Result: Notably, structural analyses show that 5 escape mutations (K417N/E484K/N501Y, G446V, F456E, N487R, F490L) are located within the interface of RBD-ACE2 interaction, indicating that this RBD subdomain is important in neutralizing SARS-CoV-2 infection.
Result: The most prevalent D614G mutation and some mutations of interest (i.e., N501Y, L452R, K417N, N439K, S477N, S494P) were among the variants printed.
Dynamic Ca(2+) sensitivity stimulates the evolved SARS-CoV-2 spike strain-mediated membrane fusion for enhanced entry.
Result: We found that Beta fusion activity is ~15% lower compared to the Alpha strain, which could be due to the lower affinity of ACE2 for B1.351 than B.1.1.7 spike variant, due to additional mutation on B.1.351 variant (K417N and E484K), consistent with a previous report.
Emergence and phenotypic characterization of the global SARS-CoV-2 C.1.2 lineage.
Method: The SARS-CoV-2 Wuhan-1 spike, cloned into pCDNA3.1, was mutated using the QuikChange Lightning Site-Directed Mutagenesis kit (Agilent Technologies) and NEBuilder HiFi DNA Assembly Master Mix (NEB) to include D614G (wild-type) or lineage defining mutations for Beta (L18F, D80A, D215G, 241-243del, K417N, E484K, N501Y, Result: High neutralization titers against D614G, C.1.2, and Delta are likely a result of higher viral loads associated with Delta infections, while reduced titers against Beta may be a result of the K417N substitution, which escapes a predominant antibody class, and is not present in Delta or C.1.2.
SARS-CoV-2 BA.1 variant is neutralized by vaccine booster-elicited serum, but evades most convalescent serum and therapeutic antibodies.
PMID: 35380448
2022
Science translational medicine
Result: We compared the neutralization titers of these serum samples against pseudoviruses bearing spike proteins from the following variants: D614G, Omicron (A67V, del69-70, T95I, del142-144, Y145D, del211, L212I, ins214EPE, G339D, S371L, S373P, S375F, K417N, N440K, G446S, S477N, T478K, E484A, Q493R, G496S, Q498R,
Multiple SARS-CoV-2 Variants Exhibit Variable Target Cell Infectivity and Ability to Evade Antibody Neutralization.
Abstract: Surprisingly, Mu+K417N mutant exhibited almost no decrease in neutralization.
Introduction: Beta+R346K and Mu+K417N share the same haplotype with R346K, K417N, E484K and N501Y mutations in the receptor-binding domain (RBD) in the spike protein, as shown in Figure 2.
Introduction: Similarly, the Mu variant (B.1.621) with spike-K417N mutation (Mu+K417N) has been identified not only in the South American countries, such as Columbia and Peru, but also in North America and European nations.
Discussion: Imdevimab exhibited efficacy against both Beta+<
Rapid identification of neutralizing antibodies against SARS-CoV-2 variants by mRNA display.
Result: Neither N501Y (the only RBD mutation in B.1.1.7 and one of 3 RBD mutations in B.1.351) nor K417N (one of 3 RBD mutations in B.1.351) disrupted the binding affinity of N-612-017.
Result: To assess the relative affinity of RBD-binding nAbs N-612-017 and N-612-056 against a series of variants, BLI was performed using RBD variants B.1.1.7 (N501Y), B.1.351 (K417N/E484K/N501Y), CAL.20C (L452R), and A.VOI.V2 (T478R/E484K) with single or combined mutations.
Neutralizing antibody responses elicited by SARS-CoV-2 mRNA vaccination wane over time and are boosted by breakthrough infection.
PMID: 35166573
2022
Science translational medicine
Introduction: Another VOC to emerge at about the same time was Beta (B.1.351), which is characterized by other NTD mutations and deletions, as well as crucial RBD mutations, including K417N, E484K, and N501Y.
Mass Screening of SARS-CoV-2 Variants using Sanger Sequencing Strategy in Hiroshima, Japan.
Method: 2 literally having K417N, T478K, E484A and N501Y mutation in the Sanger sequences.
Method: The Delta variant without mutation at nt22813 is the ordinary B.1.617.2 (Delta) but it is AY.1 or AY.2 (Delta Plus) if there is mutation from Guanine (G) to Thymine (T) at nt22813 (referred to K417N).
Discussion: The Omicron variant possess the distinct mutation pattern having both K417N (Delta) and N501Y (Alpha) in the spike region plus E484A and various mutations which can be easily identified by our Sanger Sequencing Strategy (as shown in.
SARS_CoV_2 mutation literature information.
PMID: 
2022
Theranostics
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