Result: This suggests that background cytotoxicity may have contributed to lowered BL in the absence of H84T-BanLec viral binding.
Result: We next investigated whether H84T-BanLec CAR-NK cells could specifically target S-protein pseudoviral transduced hACE2.293T.
Result: We observed a reduction in SARS-CoV-2 pseudoviral mediated bioluminescence emission from hACE2.293T cells when H84T-BanLec CAR-NK cells were present ( Figure 3D ).
Result: We produced replication incompetent retrovirus carrying our CAR sequence and used this to generate H84T-BanLec CAR NK cells.
Sensing the interactions between carbohydrate-binding agents and N-linked glycans of SARS-CoV-2 spike glycoprotein using molecular docking and simulation studies.
PMID: 33292056
2020
Journal of biomolecular structure & dynamics
Introduction: Here, in this study, we selected lectins like NPA, UDA, GRFT and CV-N, Banana lectin (BanLec) wild-type and mutant (H84T mutant) type and non-peptidic mimic PRM-A based on their previously reported antiviral activity and availability of PDB structures.
Method: Also, the various plant lectins like NPA with PDB ID: 1NPL (Sauerborn et al.,), UDA with PDB ID: 1EHD (Harata & Muraki,), BanLec of wild-type (PDB ID: 3MIT) (Sharma & Vijayan,) and H84T mutant type (PDB ID: 4PIT) (Swanson et al., 2015), lectins from algal and cyanobacterial origin like GRFT (PDB ID: 2GUD) (Ziolkowska et al.,) and CV-N with PDB ID: 3GXZ (Botos et al.,), respectively, were retrieved from the PDB databank for the macromolecular docking studies with SARS CoV-2 S glycoprotein.
SARS_CoV_2 mutation literature information.
PMID: 
2021
Frontiers in immunology
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