Discussion: Although our sample size is small, the convergent origination of S H655Y with E S67S in all index cats and the fixation of these variants following transmission in two contact cats signals a potentially important functional role for one or both of these variants in feline hosts, and points towards a potential selective bottleneck.
Discussion: Although we cannot easily test this, if the transmission bottleneck were large and S H655Y, in linkage with E S67S, were rapidly selected in contact hosts immediately following transmission we might see a similar pattern to what we observe in cats 4 and 6.
Discussion: Based on iSNV frequencies, S
Transmission of SARS-CoV-2 in domestic cats imposes a narrow bottleneck.
Abstract: Our data suggest that here, positive selection in index cats followed by a narrow transmission bottleneck may have instead accelerated the fixation of S H655Y, a potentially beneficial SARS-CoV-2 variant.
Abstract: We further identify a notable variant at amino acid position 655 in Spike (H655Y), which was previously shown to confer escape from human monoclonal antibodies.
Introduction: Here we use a cat transmission model to show that SARS-CoV-2 genetic diversity is largely shaped by genetic drift and purifying selection, with the notable exception of a single variant in Spike at residue 655 (H655Y).
Method: Focal Nextstrain build of S H655Y sequences.
Method: The focal
Resistance of SARS-CoV-2 variants to neutralization by monoclonal and serum-derived polyclonal antibodies.
Figure: Substitutions seen in the B.1.1.248 Brazilian variant (L18F, T20N, P26S, D138Y, R190S, K417T, E484K, N501Y, D614G, H655Y, T1027I, and V1176F) are shaded red.
Genomics and epidemiology of a novel SARS-CoV-2 lineage in Manaus, Brazil.
Result: Additionally, whereas a convergent A701V mutation is also found in the B.1.526 and S/E484K carrying lineage that was first identified in New York, P681H is found in the S/E484K and S/N501Y carrying P.3 lineage first identified in the Philippines, and both S/H655Y and S/P681H are found in the highly mutated S/E484K carrying A.VOI.V2 lineage first identified in Tanzanian travellers.
Result: Any of H655Y, P681H, A701V or T716I might directly impact the efficiency of viral entry into host cells.
Result: Whereas sites S/655,
SARS-CoV-2 rapidly adapts in aged BALB/c mice and induces typical pneumonia.
Result: the LG strain isolated from the lungs of aged mice had the following nucleotide mutations: (i) one synonymous variant, A8203G (nsp3, V-V); and (ii) the remaining mutations that were nonsynonymous, including T21784A (spike, NTD, N74K), A23056C (spike, RBD, Q498H), C23525T (spike, S1, H655Y), and G29573A (ORF10, I6V).
Result: the passaged virus WH-DC had the following nonsynonymous mutations: C17825T (EndoRNAse
Neutralising antibody escape of SARS-CoV-2 spike protein: Risk assessment for antibody-based Covid-19 therapeutics and vaccines.
Method: The P.1 virus used in these studies contained the following mutations: L18F, T20N, P26S, D138Y, R190S, K417T, E464K, N501Y, D614G, H655Y, T1027I, V1176F.
Result: Compared to the Wuhan sequence, P.1 contains the following mutations: L18F, T20N, P26S, D138Y, and R190S in the NTD; K417T, E484K, and PMID: 33853970
2021
Science (New York, N.Y.)
Introduction: Lineage P.1 contains 10 lineage-defining amino acid mutations in the virus spike protein (L18F, T20N, P26S, D138Y, R190S, K417T, E484K, N501Y, H655Y, and T1027I) compared with its immediate ancestor (B.1.1.28).
A Potential SARS-CoV-2 Variant of Interest (VOI) Harboring Mutation E484K in the Spike Protein Was Identified within Lineage B.1.1.33 Circulating in Brazil.
Introduction: The VOC P.1, first described in January 2021, displayed an unusual number of lineage-defining mutations in the S protein (L18F, T20N, P26S, D138Y, R190S, K417T, E484K, N501Y, H655Y, T1027I) and its emergence was associated with a second COVID-19 epidemic wave in the Amazonas state.
SARS_CoV_2 mutation literature information.
PMID: 
2021
bioRxiv
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