Method: It is the ectodomain of SARS-CoV-2 spike protein expressed in HEK293 cells, which contains amino acids Val16 - Pro1213 of Spike (GenBank: QHD43416.1) with T4 fibritin trimerization motif, a polyhistidine tag at the C-terminus, proline substitutions (F817P, A892P, A899P, A942P, K986P, V987P) and alanine substitutions (R683A and R685A) to stabilize the trimeric pre-fusion conformation, and mutations identified in the Omicron variant (A67V, HV69-70del, T95I, G142D, VYY143-145del, N211del,
Discriminatory Weight of SNPs in Spike SARS-CoV-2 Variants: A Technically Rapid, Unambiguous, and Bioinformatically Validated Laboratory Approach.
Introduction: Last in order of arrival but certainly not least, the variant called Omicron (B.1.1.529) is characterized
Result: Considering several parameters, including estimated informedness and amplicon lengths, we observed that the subset comprising the SNPs K417N, N439K, Y453F, S477N, T478K, E484K, N501Y, A570D, H655Y, Q677H, P681H, I692V, A701V, and716I, would lead to a test providing an acceptable compromise between informedness (0.76) and minimal amplicon length (896 bp).
Table: H655Y
Emergence of a novel SARS-CoV-2 Pango lineage B.1.1.526 in West Bengal, India.
PMID: 34896696
2022
Journal of infection and public health
Introduction: Along with the clade specific mutations, these variants have their own sets of characteristics mutations including several mutations in the S glycoprotein like Delta69-70, Delta144-145, N501Y, A570D, P681H, T716I, S982A, D1118H in the Alpha variant; D80A, D215G, Delta241-243, K417N, E484K, N501Y, A701V in the Beta variant; L18F, T20N, P26S, D138Y, R190S,
A monoclonal antibody that neutralizes SARS-CoV-2 variants, SARS-CoV, and other sarbecoviruses.
Result: And when we went back to sequence the viruses from earlier passages, only H655Y, which has also been found in other circulating variants such as P.1, and V1128A were found.
Result: Indeed, when these mutations were introduced into pseudoviruses and tested for their sensitivity to 2-36, only K378 T alone or in combination with the other mutations was found to be resistant to 2-36, whereas viruses with T284I, H655Y, or V1128A alone remained sensitive (Figure 4D).
Result: Sequence analyses of the passage 12 virus revealed four single point spike mutations (T284I, K378 T, H655Y, V1128A), all
Haplotype distribution of SARS-CoV-2 variants in low and high vaccination rate countries during ongoing global COVID-19 pandemic in early 2021.
PMID: 34848355
2022
Infection, genetics and evolution
Table: H655Y
Longitudinal analysis of SARS-CoV-2 spike and RNA-dependent RNA polymerase protein sequences reveals the emergence and geographic distribution of diverse mutations.
PMID: 34801754
2022
Infection, genetics and evolution
Figure: L18F, E484K, N501Y, D614G, H655Y, and V1176F are associated with the Gamma variant of concern, and S13I, W152C, L452R are associated with the Epsilon variant of concern.
The significant immune escape of pseudotyped SARS-CoV-2 variant Omicron.
Result: There are 32 mutations on the Spike of Omicron, including the following sites: A67V, H69del-V70del, T95I, G142D-V143del-Y144del-Y145del, N211del-L212I, ins214EPE, G339D, S371L, S373P, S375F, K417N, N440K, G446S, S477N, T478K, E484A, Q493R, G496S, Q498R, N501Y, Y505H, PMID: 34896524
2022
Gene
Table: H655Y
Mutation profile of SARS-CoV-2 genome in a sample from the first year of the pandemic in Colombia.
PMID: 34933126
2022
Infection, genetics and evolution
Result: The mutations p.Lys1191Asp (K1191D), p.Glu484Lys (E484K), p.Leu18Phe (L18F), p.Pro26Ser (P26S), p.His655Tyr (H655Y), p.Asp614Gly (D614G), that along with other nucleotide substitutions constitute some of the VOC and VOI, were also identified, but not necessarily defining a complete lineage of interest.
SARS_CoV_2 mutation literature information.
PMID: 
2022
Nature
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