Result: The engineered G614D Alpha spike (Y501, D614) shows the same binding affinity as Wuhan (N501, D614).
Figure: b Plots of fractional saturation binding measurements with data for the Alpha variant shown in red, Alpha variant with G614D substitution in pink, and Wuhan (D614) spike in blue.
Ferristatin II Efficiently Inhibits SARS-CoV-2 Replication in Vero Cells.
Method: The plates were incubated for 48 h (for Wuhan G614D virus) or 72 h (for Delta and Omicron viruses) at 37 C, 5% CO2, until a clear cytopathic effect was seen in positive control wells.
Whole-genome analysis of SARS-CoV-2 in a 2020 infection cluster in a nursing home of Southern Italy.
PMID: 35189404
2022
Infection, genetics and evolution
Table: G614D
SARS-CoV-2 in Egypt: epidemiology, clinical characterization and bioinformatics analysis.
Discussion: Moreover, the spike proteins (SP) of all Egyptian tested strains (n = 144) are completely identical in that they have a G to D substitution at site 614 compared with the reference strain (NC_045512).
Haplotype distribution of SARS-CoV-2 variants in low and high vaccination rate countries during ongoing global COVID-19 pandemic in early 2021.
PMID: 34848355
2022
Infection, genetics and evolution
Result: Meanwhile, haplotype 2 with the mutations at nsp12 P323L and spike G614D was almost total post-1-year spread (98.81%).
The virological impacts of SARS-CoV-2 D614G mutation.
PMID: 34289053
2021
Journal of molecular cell biology
Abstract: The current prevalent D614G variant, with glycine substituted for aspartic acid at position 614 in the spike glycoprotein, is one of such variants that became the main circulating strain worldwide in a short period of time.
Introduction and Characteristics of SARS-CoV-2 in North-East of Romania During the First COVID-19 Outbreak.
Introduction: One of the most well-known mutations is in position 23404, changing an aspartate for a glycine at residue 614 in Spike protein and, presumably, offering an advantage in viral replication.
Ongoing global and regional adaptive evolution of SARS-CoV-2.
Result: Additionally, apart from the selective advantage of a single replacement, it should be emphasized that D614G (but not G614D) is a central hub of the epistatic network.
Result: As a result, the reverse replacement G614D appears often enough to pass our statistical criteria for positive selection.
Result: As discussed above, these early mutations (including S G614D) might provide only a modest selective advantage in isolation but exert a much greater effect through multiple epistatic interactions.
Landscape-Based Mutational Sensitivity Cartography and Network Community Analysis of the SARS-CoV-2 Spike Protein Structures: Quantifying Functional Effects of the Circulating D614G Variant.
Result: In the locked closed form of S-G614 the largest destabilization changes were induced by the G614K, G614D, and G614E mutations (Supporting Information, Figure S4B), highlighting the fact that the reverse G614D mutation could significantly destabilize the S-G614 trimer.
SARS_CoV_2 mutation literature information.
PMID: 
2022
Genetics and molecular biology
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