Mortality in COVID-19 disease patients: Correlating the association of major histocompatibility complex (MHC) with severe acute respiratory syndrome 2 (SARS-CoV-2) variants.
PMID: 32693089
2020
International journal of infectious diseases
Introduction: For MHC class I, starting with a peptide with the mutation site in the middle (flanked by seven amino acid residues to the left and right, resulting in a 15mer peptide) for ORF8 (L84S) (GNYTVSCLPFTINCQ and GNYTVSCSPFTINCQ) and for ORF3a (G251V) (VQIHTIDGSSGVVNP and VQIHTIDVSSGVVNP), we tested all possible combinations of length from 8 to 14 residues for MHC class I binding, For MHC class II, starting with a peptide with 33 residues with the mutation site in the middle for ORF8 (L84S) (KSPIQYIDIGNYTVSCL/SPFTINCQEPKLGSLVV) and for ORF3a (G251V) (KIVDEPEEHVQIHTIDG/VSSGVVNPVMEPIYDEP), we tested all possible combinations to bind to MHC class II alleles listed in the supplementary data set since MHC class
Variant analysis of SARS-CoV-2 genomes.
PMID: 32742035
2020
Bulletin of the World Health Organization
Result: G251V frequently appears in samples from the United Kingdom (329 samples), Australia (95 samples), the United States (80 samples) and Iceland (76 samples).
Result: Among the L3606F subclades, L3606F/G251V/ forms the largest group with 419 samples.
Table: G251V
Geographic and Genomic Distribution of SARS-CoV-2 Mutations.
5Result: Other two major clades are called ""S,"" named after the mutation in ORF8 L84S (Ceraolo and Giorgi,), also characterized by a silent C8782T genomic mutation, and ""V,"" from the ORF3a:G251V mutation, almost always co-occurring with the NSP6:L37F event, and identified by early phylogenetic studies (Forster et al.,)."
Table: G251V
Comparative genome analysis of novel coronavirus (SARS-CoV-2) from different geographical locations and the effect of mutations on major target proteins: An in silico insight.
Result: The analysis of ORF3a showed 3 mutations within different SARS-CoV-2 strains: W128L (South Korea), L140V (Japan), G251V (Australia, South Korea, Brazil, Italy, Sweden) (Table 3).
Table: G251V
BioAider: An efficient tool for viral genome analysis and its application in tracing SARS-CoV-2 transmission.
PMID: 32904401
2020
Sustainable cities and society
Introduction: In addition, the non-synonymous substitutions of ORF3a-G251V and ORF8-L84S both cause the changes of amino acid (aa) polarity, which may affect the conformation of the protein and lead to function alteration.
Introduction: Recently, 7 substitution hotspots in SARS-CoV-2, ORF1ab-G10818T (ORF1ab-L3606F), ORF1ab-C8517T, ORF3a-G752T (ORF3a-G251V) S-A1841G (D614G), G171T (Q57H), ORF8-T251C (ORF8-L84S) and N-GGG608_609_610AAC (N-RG203_204KR) have been
Characterization of local SARS-CoV-2 isolates and pathogenicity in IFNAR(-/-) mice.
Abstract: P4715L in RdRp, G251V in ORF3a and S1498F of Nsp3 is associated with the epitope loss that may influence pathogenesis caused by antibody escape variants.
Result: The incidence of occurrence of G251V is high among various samples irrespective of geographical locations.
Result: The second epitope loss is associated with G251V in ORF3a.
Figure: (b) Epitope loss linked with ORF3a G251V.
Figure: G251V mutant (right) causes loss of DGSSGVV(250....256aa).
Discussion: SARS-CoV-2 genome sequences available at the pu
Geographical Distribution of Genetic Variants and Lineages of SARS-CoV-2 in Chile.
Introduction: The genetic variants are located in the nucleotide positions 23,403, 26,144, and 28,144 based on the reference sequence NC_045512.2, and the variant's name is represented by a capital letter that corresponds to the amino acid substitution product of the SNP G: Spike:D614G, V: NS3:G251V, S: ORF8:L84S, respectively, and O for other strains that keep some of the nucleotide as the reference strain on that genome position that cannot be assigned to the previous described clades.
New Pathways of Mutational Change in SARS-CoV-2 Proteomes Involve Regions of Intrinsic Disorder Important for Virus Replication and Release.
Abstract: Notable expanding mutations include R203K and G204R of the nucleocapsid (N) protein inter-domain linker region and G251V of the viroporin encoded by ORF3a between March and April.
Figure: A comparison of the different mutants and reference viral strain with a delta score revealed that mutations G196V and G251V decreased disorder.
Discussion: In addition, a comparison of mutants V13L, Q57H, G196V and G251V and the reference viral strain with delta scores of disorder and binding revealed interesting patterns.
Discussion: In contrast, PMID: 33170902
2020
PloS one
Result: C1 is also composed of two subclades, SCB 1 sharig the mutation G251V (ORF3a) first identified in strains from china and further emerged in European strains, such as England and Iceland.
Result: For the four other hotspot mutations were distributed in ORF3a (Q57H and G251V) and nucleocapsid phosphoprotein (R203K and G204R).
Result: However, among the recurrent non-synonymous, synonymous, deletion and intergenic mutations, we found G251V (in ORF3a), and S5932F (in ORF1ab) present on all continents except Africa (Fig 3).
SARS_CoV_2 mutation literature information.
PMID: 
2020
International journal of infectious diseases
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