5Result: Concordantly, the first D839Y genome sequence reported worldwide was collected in Italy (Lombardy) on 21 February (Italy/PV-5314-N/2020; GISAID accession number EPI_ISL_451307) (Table S2), being identical (i.e, Nextstrain clade 20A background plus the G24077 T SNP) to the ""founder"" Spike Y839 variant genome sequences detected in Portugal (https://insaflu.insa.pt/covid19)."

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5Result: In this particular case, we cannot exclude the hypothesis that the G24077 T nucleotide change might have been introduced in a clade ""20C"" SARS-CoV-2 by recombination, as ""20C"" and ""20A harbouring Y839"" viruses co-circulated in Wales during the collection period (13 out of the 51 Spike 839Y genomes from UK were collected in Wales between 10 April and 25 May)."

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Result: Of those, 92 genomes revealed the same