Community-level SARS-CoV-2 sequence diversity revealed by wastewater sampling.
PMID: 34438144
2021
The Science of the total environment
Result: Consistent with this study, we identified R203K, G204R, S194L, and M234I in the nucleocapsid protein as shared mutations between Columbia and Rock Hill WWTP influents in January 2021 (Supplementary Table S3).
Result: In particular, R203K and G204R both had a mutation rate of 0.22, indicating that they were present in 22% of all sequences as of December 2020.
Novel Nested-Seq Approach for SARS-CoV-2 Real-Time Epidemiology and In-Depth Mutational Profiling in Wastewater.
PMID: 34445204
2021
International journal of molecular sciences
Result: Consequently, the absence of R203 residue is expected to have an immediate impact on the N protein folding and functionality, while any of the variations in position 204 of the protein (G204R, G204L, or G204H) lead only to subtle modifications (Figure 5).
Molecular characterization of SARS-CoV-2 from Bangladesh: implications in genetic diversity, possible origin of the virus, and functional significance of the mutations.
Abstract: We identified multiple missense mutations in B-cell and T-cell predicted epitope regions and/or PCR target regions (including R203K and G204R that occurred in 86% of the isolates) that may impact immunogenicity and/or RT-PCR based diagnosis.
Result: About 88% of the sequences clustered in GR clade the distinctive feature of which is G204R mutation in the nucleocapsid protein (Figure 4 and Table 3).
Result: Beside these, two of the high frequency (86%) SNVs (R203K and G204R) occurred in COVID-19 diagnostic RT-PCR target and B-Cell predicted epitope regions, of which the later was predicted to cause altered function of the nucleocapsid
Table: G204R
Delta spike P681R mutation enhances SARS-CoV-2 fitness over Alpha variant.
Diagnostic pre-screening method based on N-gene dropout or delay to increase feasibility of SARS-CoV-2 VOC B.1.1.7 detection.
PMID: 34464903
2021
Diagnostic microbiology and infectious disease
Result: Mutations detected in the N protein of all samples analyzed were M1X, D3L, R203K, G204R and S235F.
Discussion: Other mutations detected in all samples analyzed, such as M1X, R203K and G204R, could affect the amplification efficiency of the N-gene target.
Genome-wide identification and prediction of SARS-CoV-2 mutations show an abundance of variants: Integrated study of bioinformatics and deep neural learning.
PMID: 34812411
2021
Informatics in medicine unlocked
Discussion: Along with the previously found GGG > AAC mutation, our mutational type analysis identify some another multi-nucleotide mutations CC > TT, TG > CA, and AT > TA which are in the top 20 mutational type and should be monitored for the future as the GGG > AAC (R203K and G204R) reported to be associated with the insertion of a lysine in SR domain of N protein which might affect the phosphorylation.
Characterization of altered genomic landscape of SARS-CoV-2 variants isolated in Saudi Arabia in a comparative in silico study.
PMID: 34866979
2021
Saudi journal of biological sciences
Result: Other changes which were frequently identified include G25563T (G57H) and C26735T (silent) each of which occurred in 33 variants, followed by C18877T (silent), G28881A (S202N) and G28883C (G204R) which appeared in 32, 23 and 22 variants respectively.
Discussion: All structural genes in our study showed missense mutations except E gene which showed only one silent mutation; N gene showed highest mutations among the structural genes, the most frequent mutations at positions G28881A (S202N), and G28883C (
Generation of a novel SARS-CoV-2 sub-genomic RNA due to the R203K/G204R variant in nucleocapsid: homologous recombination has potential to change SARS-CoV-2 at both protein and RNA level.
Discussion: The recently evolved Indian lineages B.1.617.1 (Kappa), B.1.617.2 (Delta), and AY.1 (Delta-plus) also carry a point mutation at 28,881, which is a novel N mutation, R203M, instea
Discussion: Using the authentic virus, we proved that R203K/G204R increase viral replication, which enhances the infectivity, fitness, and virulence of SARS-CoV-2.
Discussion: We found that the increase in infectivity was due to the promotion of virus replication efficiency, which may have been caused by the change in the local charge of the N protein resulting from the R203K/G204R mutations.
Discussion: We have performed analyses and experiments on R203K/G204R mutant virus.
SARS-CoV-2 Omicron has extensive but incomplete escape of Pfizer BNT162b2 elicited neutralization and requires ACE2 for infection.
Method: P2 stock was sequenced and confirmed Omicron with the following substitutions: E:T9I,M:D3G,M:Q19E,M:A63T,N:P13L,N:R203K,N:G204R,ORF1a:K856R,ORF1a:L2084I,ORF1a:A2710T,ORF1a:T3255I,ORF1a:P3395H,
ViMRT
SARS_CoV_2 mutation literature information.
PMID: 
2021
The Science of the total environment
