Substitutions in Spike and Nucleocapsid proteins of SARS-CoV-2 circulating in South America.
PMID: 32950697
2020
Infection, genetics and evolution
Result: In the N protein, five amino acid substitutions were found; the most frequent being R203K and G204R in 13.95% (6/43) of the sequences.
Result: Non-synonymous substitutions R203K and G204R, which are the hallmarks of the B.1.1 lineage, were the most frequent in the N protein of South American sequences.
Result: The most frequent in S were D614G (83%) V1176F (2.2%) and P126
Discussion: On the other hand, the co-occurrence of R203K and G204R substitutions in the N protein, was identified in 34% of South American sequences.
A persistently replicating SARS-CoV-2 variant derived from an asymptomatic individual.
PMID: 32967693
2020
Journal of translational medicine
Abstract: Whole genome sequencing of the persistently replicating SARS-CoV-2 GZ69 has shown that this strain differs from the early AP66 variant in 9 nucleotide positions (C2939T; C3828T; G21784T; T21846C; T24631C; G28881A; G28882A; G28883C; G29810T) which lead to 6 non-synonymous substitutions spanning on ORF1ab (P892S; S1188L), S (K74N; I95T) and N (R203K,
Coding-Complete Genome Sequences of Three SARS-CoV-2 Strains from Bangladesh.
Introduction: Compared with hCoV-19/Wuhan/WIV04/2019, for strain BCSIR-NILMRC-006, we found eight mutations, including NSP2_G339S, N_R203K, N_G204R, NSP3_Q172R, Spike_D614G, NSP2_I120F, NSP12_P323L, and NSP2_V480I.
Introduction: In BCSIR-NILMRC-008, the genome mutations Spike_D614G, N_R203K, N_G204R, NSP2_
Non-synonymous mutations of SARS-CoV-2 leads epitope loss and segregates its variants.
Discussion: R203K and G204R in nucleocapsid protein are found in SARS-CoV-2 of three Indian patients who traveled from Italy in April 2020.
Discussion: SARS-CoV-2 genome sequences available at the public database of the Global Initiative on Sharing All Influenza Data (GISAID) till 02-10-2020 classifies the variants into several clades like (i) L-original lineage, (ii) G-variant of spike protein causing D614S mutation, (iii) S- variant ORF8 responsible for L84S mutation, (iv) V- variant of the ORF3a coding protein N3S responsible for G251V mutation, (v) GH- a G derivative characterized by ORF3a: PMID: 33093050
2020
Microbiology resource announcements
Introduction: Four amino acid substitutions (ORF1b-P314L, S-D614G, N-R203K, and N-G204R) unique to clade GR (both lineages B.1.1 and B.1.1.28) were seen in all our sequences in this clade.
Table: G204R
New Pathways of Mutational Change in SARS-CoV-2 Proteomes Involve Regions of Intrinsic Disorder Important for Virus Replication and Release.
Abstract: Notable expanding mutations include R203K and G204R of the nucleocapsid (N) protein inter-domain linker region and G251V of the viroporin encoded by ORF3a between March and April.
Figure: A similar outcome was obtained with the G204R mutant.
Discussion: The R203K and G204R mutations spanned regions of high disorder and high binding ability.
Discussion: The coordinated rates of increase of the R203K and G204R mutations indicated mutations did not occur randomly.
SARS-CoV-2 spread across the Colombian-Venezuelan border.
PMID: 33157300
2020
Infection, genetics and evolution
Abstract: Additionally, three mutations (R203K/G204R substitution) were present in the nucleocapsid (N) gene of one Venezuelan genome.
Result: Additionally, we identified three substitutions in the nucleocapsid (N) gene of VEN-89312 changing GGG-to-AAC at positions 28,616-28,618 resulting R203K/G204R substitutions according to the whole-genome position after removing the 5'UTR.
Result: These R203K/G204R substitutions, which have been reported previously in other South American genomes, were absent from the two other Venezuelan genomes we sequenced.
Genomic exploration light on multiple origin with potential parsimony-informative sites of the severe acute respiratory syndrome coronavirus 2 in Bangladesh.
Abstract: Genome-wide annotations revealed 256 mutations, of which 10 were novel (NSP3, RdRp, Spike) in Bangladeshi strains where I120F(NSP2), P323L(RdRp), D614G (Spike), R203K, G204R(N) are the most prominent.
Large scale genomic analysis of 3067 SARS-CoV-2 genomes reveals a clonal geo-distribution and a rich genetic variations of hotspots mutations.
Result: For the four other hotspot mutations were distributed in ORF3a (Q57H and G251V) and nucleocapsid phosphoprotein (R203K and G204R).
Result: SARS-CoV-2 genomes also harbored three co-occurrent mutations R203K, R203R and G204R in the N protein and were present in all continents except Africa and Asia (besides Taiwan).
Result: The first subcluster SCB3 harbored strain sharing two mutation R203K (N) and G204R (N) harboring largely strains from Europe and some strains from North Africa (Franc
Analysis of genomic distributions of SARS-CoV-2 reveals a dominant strain type with strong allelic associations.
Result: In addition to the three type VI signature SNVs, five additional subtype SNVs with variation frequencies of >0.1 included C1059T (nsp2, T265I), G25563T (ORF3a, Q57H), G28881A (N, R203K), G28882A (N, R203K), and G28883C (N, G204R).
SARS_CoV_2 mutation literature information.
PMID: 
2020
Infection, genetics and evolution
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