Abstract: We expressed Mpro of six SARS-CoV-2 lineages (C.37 Lambda, B.1.1.318, B.1.2, B.1.351 Beta, B.1.1.529 Omicron, P.2 Zeta), each of which carries a strongly prevalent missense mutation (G15S, T21I, L89F, K90R, P132H, L205V).
Introduction: Hence, we selected the six mutations G15S, T21I, L89F, Introduction: WT SARS-CoV-2 Mpro and the mutants G15S, T21I, L89F, K90R, P132H and L205V were expressed in E.
Structural basis for the in vitro efficacy of nirmatrelvir against SARS-CoV-2 variants.
PMID: 35461811
2022
The Journal of biological chemistry
Table: G15S
Figure: (a) Analytical size-exclusion profile using using Zenix SEC-300, 4.6 x 150 mM column (b) SDS-PAGE analysis: Lane 1, Marker; Lane 2, SARS-Cov2-Mpro wildtype; Lane 3, SARS-Cov2-Mpro G15S; Lane 4, SARS-Cov2-Mpro K90R; Lane 5, SARS-Cov2-Mpro P132H (c) Enzyme Kinetics of Mpro variants: The rate of cleavage of the FRET peptide substrate in the presence of indicated Mpro is monitored by increase in fluorescence over time with the fluorescent signal being converted to nM substrate cleaved by use of a standard curve generated from cleaved substrate.
Figure: Residual deuterium exchange plots indicate no significant differential uptake between wild-type and SARS-CoV-2-Mpro mutants; (a.) G15S, (b.) K90R and (c.) P132H.
Discussion:
Variant analysis of SARS-CoV-2 genomes in the Middle East.
SARS-CoV-2 main protease suppresses type I interferon production by preventing nuclear translocation of phosphorylated IRF3.
PMID: 33907518
2021
International journal of biological sciences
Abstract: NSP5 variants G15S and K90R commonly seen in circulating strains of SARS-CoV-2 retained the IFN-antagonizing property.
Result: G15S and K9
Result: Notably, G15S is a novel substitution only seen in SARS-CoV-2, whereas K90R has also been observed in SARS-CoV.
Result: To determine whether the non-synonymous substitutions might affect type I IFN antagonism of SARS-CoV-2 NSP5, we created its G15S and K90R variants.
Result: Whereas the G15S variant is common in European strains, the K90R variant is more frequently found in Chinese and Icelandic strains.
Impact of meteorological parameters and population density on variants of SARS-CoV-2 and outcome of COVID-19 pandemic in Japan.
Result: Among 22 point mutations at N and other eight non-structural proteins, eight namely, N_S194L, N_R203K, N_G204R, NS3_Q57H, NSP2_T85I, NSP5_G15S, NSP6_L37F and NSP12_P323L were persistent throughout the COVID-19 pandemic in Japan.
Result: Significant correlation was detected between NSP5_G15S and UV (rs = 0.91), followed by NS8_S24L and maximu
Global variation in SARS-CoV-2 proteome and its implication in pre-lockdown emergence and dissemination of 5 dominant SARS-CoV-2 clades.
PMID: 34147651
2021
Infection, genetics and evolution
Result: Some notable substitutions, co-occurring mutations, deletions and insertions which occur with a low to moderate recurrence and may have a significant influence on the viral pathogenic mechanism are: co-deletion (dependent) of K141, S142 and F143, co-deletion (dependent) of G82 and H83, D75E, co-occurring (dependent) S135N, Y136 deletion and M85 deletion in Nsp1, D268 deletion, G212D, co-occurring (dependent) I559V and P585S, F10L, V198I, P91S, T166I, H237R, T371I, S211F and G339S in Nsp2, PMID: 34281387
2021
mBio
Abstract: In addition, a retention of Q57H (B.1.X), R203K/G204R (B.1.1.X), T85I (B.1.2-B.1.3), G15S+T428I (C.X), and I120F (D.X) variations was observed.
Result: Amino acid substitutions such as T428I and G15S in ORF1a were reported in sublineages C.1 and C.2, and the S477N substitution in the spike (S) protein along with I120F in nsp2 specifically established the sublineage D.2.
Result: Other major mutations noted are Q57H
Geographic and Genomic Distribution of SARS-CoV-2 Mutations.
Result: The G15S mutation in the viral protease NSP5 is the 16th most common event worldwide, with 1,798 samples affected (3.7%), however it seems to be too peripheric, in the protein sequence, to influence catalytic activity, and folding (Zhang et al.,).
SARS_CoV_2 mutation literature information.
PMID: 
2022
Infection, genetics and evolution
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