Introduction: In March 2021, genomic sequencing of SARS-CoV-2 samples in Maharashtra, India revealed an increased prevalence of E484Q, L452R and P681R co-mutation in the Spike glycoprotein (S protein).
Introduction: The S protein E484Q mutation in the Kappa variant was similarly found in the Beta (B.1.351) and Gamma (P.1) lineages, where residue 484 is mutated to lysine (E484K).
Discussion: For example, the L452R mutation, mutations at position 484 (E484K and E484Q) and mutations within the N3 loop in the NTD (residues 141-156) have all been previously characterised for their antibody-evasive effects
Drastic decline in sera neutralization against SARS-CoV-2 Omicron variant in Wuhan COVID-19 convalescents.
Introduction: Different spike mutations emerged and became dominant in the emerged variants of concern (VOC), such as the representative mutations D614G, N501Y, E484Q/K, L452R, P681R in the Alpha, Beta, Gamma, and Delta variants.
In vitro data suggest that Indian delta variant B.1.617 of SARS-CoV-2 escapes neutralization by both receptor affinity and immune evasion.
Discussion: Likewise point mutations conferred by L452R or E484Q alone have been shown to reduce sensitivity to antibodies elicited by BNT162b2 vaccine.
Discussion: More interestingly though, inhibition of RBD-ACE2 interaction by vaccine-induced antibodies significantly falls for all mutants, most markedly for E484K and L452R/E484Q (Figure 3D), indicating that the increased receptor affinity renders neutralization more difficult.
Discussion: Our data show that the affinity of RBD for ACE2 is fivefold increased by the point mutations L452R and E484Q (Figure 2C and Table 1), whereas mutation N440K has the effect of doubling this valu
Discriminatory Weight of SNPs in Spike SARS-CoV-2 Variants: A Technically Rapid, Unambiguous, and Bioinformatically Validated Laboratory Approach.
Introduction: The most recent variants, referred to as the Kappa and the Delta variants, are characterized by six mutations (E154K, L452R, E484Q, D614G, P681R, and Q1071H), and eight mutations (T19R, del156/157, R158G, L452R, T478K, P618R, D614G, and D950N) on the spike gene, respectively.
Genomic characterization unravelling the causative role of SARS-CoV-2 Delta variant of lineage B.1.617.2 in 2nd wave of COVID-19 pandemic in Chhattisgarh, India.
Abstract: More detailed analysis disclosed that the prevailing lineage in distribution is a novel identified lineage B.1.617 holding in common signature mutations D111D, G142D, L452R, E484Q, D614G, and P681R, in the spike protein, containing within the receptor-binding domain (RBD) [2, 3].
Introduction: It was first classified in India in December 2020 and quickly established itself as the most common lineage within the country, leading to an ultimate increase in the number of cases and daily deaths and overburdening of health systems in April 2021 More detailed analysis disclosed that the prevailing lineage in distribution is a novel identified lineage B.1.617 holding in common signature mutations PMID: 35080377
2022
Analytical chemistry
Conclusion: The antibodies against N501Y, E484K, and L452R/E484Q
Introduction: Each test can detect six targets (RBD, D614G, N501Y, E484K, L452R/E484Q-mutants, and 1 blank) of seven samples.
Introduction: The RBDs of SARS-CoV-2 spike protein and its variants (E484K, N501Y, D614G, and L452R/E484Q-mutants) were used as an ELISA antigen to enhance the specificity of our assay (Figure 1b).
Pan-SARS neutralizing responses after third boost vaccination in non-human primate immunogenicity model.
Result: 2 , D35 GMTs against variants with the E484K/Q mutation were lower than those against D614 by 13.9- and 34.8-fold to Beta and by 8.4- and 20.5-fold to Gamma for Groups 1 and 2, respectively.
Result: At D35, neutralizing titers against Alpha, Delta, and Epsilon variants, without the key E484K/Q mutation, were comparable to those against D614 with GMTs to D614, Alpha, and Epsilon all around the order of 3.3-3.5 log10.
Result: For the variants with the Result: collectively demonstrated that the third booster vaccination, even with the vaccines containing the original D614 Spike protein sequence, can effectively improve the quality of vaccine-induced neutralizing antibody responses by expanding the breadth of coverage against VoC, especially those harboring the key E484K/Q mutation.
Result: Third, among the top 25 most observed RBD mutations, Y449S, S494P, K417N, F490S, L452R, E484K, K417T, E484Q, L452Q, and N501Y are the 10 most antibody disruptive ones, judged by their interactions with 130 antibodies shown in Figure 1c.
Table: E484Q
Emergency SARS-CoV-2 Variants of Concern: Novel Multiplex Real-Time RT-PCR Assay for Rapid Detection and Surveillance.
Abstract: This multiplex PCR typing method was established to detect 9 mutations with specific primers and probes (DeltaHV 69/70, K417T, K417N, L452R, E484K, E484Q, N501Y, P681H, and P681R) against the receptor-binding domain of the spike protein of SARS-CoV-2 variants.
Table: E484Q
Figure: (A) VOI (K417T and P681H) and VOC (DeltaHV69-79, K417N, L452R, E484K, E484Q, N501Y, and
ViMRT
SARS_CoV_2 mutation literature information.
PMID: 
2022
Nature communications
